Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IC757_RS04650 Genome accession   NZ_CP061368
Coordinates   1063446..1064306 (-) Length   286 a.a.
NCBI ID   WP_190976211.1    Uniprot ID   A0A7H4GPX5
Organism   Wenzhouxiangella sp. AB-CW3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058446..1069306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC757_RS04630 (IC757_04630) - 1058594..1061581 (+) 2988 WP_190976207.1 DEAD/DEAH box helicase -
  IC757_RS04635 (IC757_04635) - 1061676..1062086 (-) 411 WP_190976208.1 UrcA family protein -
  IC757_RS04640 (IC757_04640) - 1062294..1062902 (-) 609 WP_190976209.1 hypothetical protein -
  IC757_RS04645 (IC757_04645) - 1062903..1063124 (-) 222 WP_190976210.1 hypothetical protein -
  IC757_RS04650 (IC757_04650) pilD 1063446..1064306 (-) 861 WP_190976211.1 prepilin peptidase Machinery gene
  IC757_RS04655 (IC757_04655) pilC 1064402..1065622 (-) 1221 WP_223846255.1 type II secretion system F family protein Machinery gene
  IC757_RS04660 (IC757_04660) pilB 1066459..1068180 (-) 1722 WP_223846256.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC757_RS04665 (IC757_04665) - 1068421..1069275 (+) 855 WP_190976212.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31461.37 Da        Isoelectric Point: 6.8191

>NTDB_id=482123 IC757_RS04650 WP_190976211.1 1063446..1064306(-) (pilD) [Wenzhouxiangella sp. AB-CW3]
MIEVIQALHPAVLVAAVFVFGLLIGSFLNVVILRYPRRLLHDWQCQCRELLELEPPTDQPPPDLVKSRSHCPSCRNTIAW
YDNIPVISWIVLGRKCRHCGTSIAFRYPLVELATAILSAVVIWHFGASTEGLAALVLTWTLIAATGIDIDHQLLPDQVTL
PLLWLGLALNLWLGLFATLEDAVIGAIAGYGSLWLVYHGFRLATGKEGMGYGDFKLLGALGAWLGWQALPMIILGASLVG
AIVGLVLMVAMRRGREIPIAFGPYLAAAGWIAMIWGDRILDFWLVY

Nucleotide


Download         Length: 861 bp        

>NTDB_id=482123 IC757_RS04650 WP_190976211.1 1063446..1064306(-) (pilD) [Wenzhouxiangella sp. AB-CW3]
ATGATCGAAGTTATCCAGGCATTGCACCCGGCGGTCCTTGTGGCCGCCGTTTTCGTTTTCGGGTTGTTGATCGGCAGCTT
TCTCAATGTGGTCATCCTGCGCTATCCGCGCCGGCTGCTGCATGACTGGCAATGCCAGTGCCGTGAGCTGCTCGAACTGG
AGCCGCCAACCGACCAGCCGCCGCCCGACCTGGTGAAATCGCGTTCGCATTGCCCATCGTGCCGGAACACGATTGCCTGG
TACGACAATATCCCGGTAATCAGCTGGATCGTGCTGGGTCGCAAATGCCGGCATTGCGGTACGAGCATCGCCTTTCGCTA
TCCGCTGGTGGAACTGGCCACGGCCATTCTCTCCGCGGTGGTCATCTGGCATTTCGGGGCCAGTACCGAAGGCCTGGCCG
CGCTGGTGCTGACCTGGACCCTGATTGCGGCAACCGGCATCGATATCGATCATCAGCTCCTGCCCGATCAGGTCACCCTG
CCCCTGCTCTGGCTGGGCCTGGCCCTGAACCTGTGGCTGGGGCTGTTTGCCACGCTGGAGGATGCCGTTATCGGCGCGAT
TGCCGGCTATGGCTCGCTGTGGCTGGTCTATCACGGTTTCCGACTGGCCACTGGCAAGGAGGGGATGGGCTACGGCGACT
TCAAGCTGCTCGGCGCACTGGGCGCCTGGCTGGGCTGGCAGGCGCTGCCCATGATCATCCTGGGCGCCTCGCTGGTCGGT
GCCATTGTTGGCCTGGTCCTGATGGTCGCCATGCGTCGGGGTCGCGAGATCCCCATCGCTTTCGGACCCTACCTGGCCGC
GGCCGGGTGGATCGCGATGATCTGGGGTGACAGAATTCTTGATTTCTGGCTGGTGTATTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-125)

Type IV leader peptidase family (Peptidase_A24)

(135-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H4GPX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

50.523

100

0.507
  pilD Acinetobacter nosocomialis M2

51.282

95.455

0.49
  pilD Acinetobacter baumannii D1279779

51.282

95.455

0.49
  pilD Vibrio cholerae strain A1552

48.936

98.601

0.483
  pilD Neisseria gonorrhoeae MS11

47.794

95.105

0.455