Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ICJ55_RS08875 Genome accession   NZ_CP061280
Coordinates   1795150..1796541 (-) Length   463 a.a.
NCBI ID   WP_188156476.1    Uniprot ID   A0A7H1C1J2
Organism   Mannheimia bovis strain ZY190616     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1790150..1801541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICJ55_RS08845 (ICJ55_08845) lptE 1790914..1791402 (+) 489 WP_188156471.1 LPS assembly lipoprotein LptE -
  ICJ55_RS08850 (ICJ55_08850) holA 1791444..1792466 (+) 1023 WP_188156472.1 DNA polymerase III subunit delta -
  ICJ55_RS08855 (ICJ55_08855) yacG 1792467..1792652 (-) 186 WP_025236197.1 DNA gyrase inhibitor YacG -
  ICJ55_RS08860 (ICJ55_08860) coaE 1792634..1793269 (-) 636 WP_188156473.1 dephospho-CoA kinase -
  ICJ55_RS08865 (ICJ55_08865) - 1793301..1793957 (-) 657 WP_188156474.1 prepilin peptidase -
  ICJ55_RS08870 (ICJ55_08870) pilC 1793961..1795157 (-) 1197 WP_188156475.1 type II secretion system F family protein Machinery gene
  ICJ55_RS08875 (ICJ55_08875) pilB 1795150..1796541 (-) 1392 WP_188156476.1 GspE/PulE family protein Machinery gene
  ICJ55_RS08880 (ICJ55_08880) pilA 1796562..1797044 (-) 483 WP_188156477.1 prepilin peptidase-dependent pilin Machinery gene
  ICJ55_RS08885 (ICJ55_08885) radA 1797226..1798605 (+) 1380 WP_188156478.1 DNA repair protein RadA -
  ICJ55_RS08890 (ICJ55_08890) - 1798659..1798889 (-) 231 WP_025236204.1 YgjV family protein -
  ICJ55_RS08895 (ICJ55_08895) - 1799016..1799504 (-) 489 WP_188156479.1 dihydrofolate reductase -
  ICJ55_RS08900 (ICJ55_08900) proB 1799616..1800707 (+) 1092 WP_188156480.1 glutamate 5-kinase -
  ICJ55_RS08905 (ICJ55_08905) - 1800707..1801471 (+) 765 WP_188156481.1 TSUP family transporter -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52383.12 Da        Isoelectric Point: 5.6550

>NTDB_id=481471 ICJ55_RS08875 WP_188156476.1 1795150..1796541(-) (pilB) [Mannheimia bovis strain ZY190616]
MSYSVTDLANQEMIEISSEQWQKNCNEKQILLRYLAVPLQETQSQLWLAVDDMQNISACEIFAFIYHKQIEPVLISSDEL
KFLLNALSPEQSSLQSEIYEDNVAYSLAHSEQIDRNDPIIQILDNLFKFCLKNNASDIHLEPQKEKLLIRLRIDGVLHIY
KTLPQSLSSRLISRIKLLAKLDISETRLPQDGQFHFKTILAEILDFRVSTLPTHLGEKVVLRLQKNKPTNLDFLELGFNQ
NQKETLLNALNQPQGLILVTGPTGSGKSITLYSALSHLNTSEKHILTVEDPIEIEIEGIIQTQVNRGINLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEFELKNALLLVVAQRLLRRTCTKCGGI
SCEHCYQGYKGRIGVYQLFSRSAKNFEKTTACLDFETLAESAKEKLAEGKTNQAEIFRVLGNG

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=481471 ICJ55_RS08875 WP_188156476.1 1795150..1796541(-) (pilB) [Mannheimia bovis strain ZY190616]
ATGTCGTATTCAGTTACCGATTTAGCCAACCAAGAAATGATTGAAATTTCGAGCGAGCAGTGGCAGAAAAATTGCAATGA
AAAACAGATTTTATTGCGATATTTGGCTGTGCCACTTCAAGAAACACAATCTCAATTATGGTTGGCGGTAGACGATATGC
AAAATATCAGTGCTTGTGAGATTTTTGCCTTTATCTACCATAAGCAAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTTTCTGCTCAATGCCCTTTCACCCGAACAGAGTAGTTTGCAGAGTGAAATTTATGAGGATAATGTAGCGTATTCATT
AGCACATTCGGAGCAAATAGACAGAAATGATCCGATTATCCAAATTTTAGATAATCTGTTTAAATTCTGTTTAAAAAATA
ACGCTTCAGATATTCATCTTGAACCGCAAAAAGAGAAATTACTCATTCGGCTGAGAATTGATGGCGTGCTGCATATTTAT
AAAACACTCCCACAAAGTTTATCGAGTAGACTGATTTCTCGCATTAAATTATTGGCTAAATTAGATATTAGTGAAACCCG
TTTACCACAAGATGGGCAATTTCACTTCAAAACAATTTTAGCGGAAATCTTAGATTTCCGAGTTTCAACTTTACCTACCC
ATTTGGGTGAAAAAGTCGTCCTTCGGCTTCAAAAGAATAAACCGACTAATTTGGATTTTCTGGAGTTAGGTTTTAACCAA
AACCAAAAAGAAACATTACTCAATGCACTCAACCAACCTCAAGGATTAATTTTAGTAACAGGTCCAACAGGTAGCGGAAA
AAGTATCACTTTATATAGTGCATTAAGCCATCTTAATACTAGCGAAAAGCATATATTAACGGTAGAAGATCCGATTGAAA
TTGAGATAGAAGGTATTATTCAAACCCAAGTAAACAGGGGCATTAATCTTGATTTCAGCCAACTGTTGAGAACCTTTCTC
CGTCAAGATCCAGACATCATTATGCTTGGGGAAATTCGTGATGAAGAGAGTGCGGAAATTGCCTTGAGAGCGGCTCAAAC
AGGGCATTTAGTGCTTTCAACATTGCATACCAATGATGCTCCTTCTGCAATTGAGCGATTGTTGCAACTCGGGATAAAAG
AATTCGAGCTGAAAAATGCGTTATTGCTTGTGGTGGCTCAACGTTTATTAAGACGAACTTGCACAAAATGTGGTGGCATT
AGTTGTGAGCATTGTTATCAGGGTTATAAAGGCAGAATTGGGGTGTATCAACTATTCAGTCGATCTGCAAAAAATTTTGA
AAAAACGACCGCTTGTTTAGATTTTGAAACCTTAGCTGAAAGTGCGAAAGAAAAATTAGCGGAAGGAAAAACCAATCAGG
CAGAAATTTTTAGGGTGTTAGGTAATGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)

Type II/IV secretion system protein (T2SSE)

(131-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1C1J2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

71.087

99.352

0.706
  pilB Haemophilus influenzae 86-028NP

57.269

98.056

0.562
  pilB Haemophilus influenzae Rd KW20

57.143

98.272

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

40.161

100

0.432
  pilB Vibrio cholerae strain A1552

41.079

100

0.428
  pilB Vibrio campbellii strain DS40M4

40.664

100

0.423
  pilB Legionella pneumophila strain ERS1305867

39.344

100

0.415
  pilB Acinetobacter baumannii D1279779

36.253

100

0.384
  pilB Acinetobacter baylyi ADP1

36.401

100

0.384