Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ICJ55_RS08880 Genome accession   NZ_CP061280
Coordinates   1796562..1797044 (-) Length   160 a.a.
NCBI ID   WP_188156477.1    Uniprot ID   A0A7H1C1J3
Organism   Mannheimia bovis strain ZY190616     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1791562..1802044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICJ55_RS08855 (ICJ55_08855) yacG 1792467..1792652 (-) 186 WP_025236197.1 DNA gyrase inhibitor YacG -
  ICJ55_RS08860 (ICJ55_08860) coaE 1792634..1793269 (-) 636 WP_188156473.1 dephospho-CoA kinase -
  ICJ55_RS08865 (ICJ55_08865) - 1793301..1793957 (-) 657 WP_188156474.1 prepilin peptidase -
  ICJ55_RS08870 (ICJ55_08870) pilC 1793961..1795157 (-) 1197 WP_188156475.1 type II secretion system F family protein Machinery gene
  ICJ55_RS08875 (ICJ55_08875) pilB 1795150..1796541 (-) 1392 WP_188156476.1 GspE/PulE family protein Machinery gene
  ICJ55_RS08880 (ICJ55_08880) pilA 1796562..1797044 (-) 483 WP_188156477.1 prepilin peptidase-dependent pilin Machinery gene
  ICJ55_RS08885 (ICJ55_08885) radA 1797226..1798605 (+) 1380 WP_188156478.1 DNA repair protein RadA -
  ICJ55_RS08890 (ICJ55_08890) - 1798659..1798889 (-) 231 WP_025236204.1 YgjV family protein -
  ICJ55_RS08895 (ICJ55_08895) - 1799016..1799504 (-) 489 WP_188156479.1 dihydrofolate reductase -
  ICJ55_RS08900 (ICJ55_08900) proB 1799616..1800707 (+) 1092 WP_188156480.1 glutamate 5-kinase -
  ICJ55_RS08905 (ICJ55_08905) - 1800707..1801471 (+) 765 WP_188156481.1 TSUP family transporter -

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 17151.50 Da        Isoelectric Point: 8.7727

>NTDB_id=481472 ICJ55_RS08880 WP_188156477.1 1796562..1797044(-) (pilA) [Mannheimia bovis strain ZY190616]
MQKIVHFRPLYTRQLHKAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLRASASYKSDVELCIYNSNGLDNCSG
GTNGIQANKTSGNETKYLNSITVTKGIITVTGKGSLEGYSYTLTPKFANNTISWSSSCSGKDTSLFPAGFCNTASAASND

Nucleotide


Download         Length: 483 bp        

>NTDB_id=481472 ICJ55_RS08880 WP_188156477.1 1796562..1797044(-) (pilA) [Mannheimia bovis strain ZY190616]
ATGCAAAAAATTGTCCATTTTCGACCGCTTTACACTCGTCAATTACACAAGGCGTTTACCTTAATTGAGTTGATGATCGT
GATTGCGATCATTGCCATTCTTGCAACCATTGCAATTCCGTCCTACAACTCTTATACGCAAAAAGCAGCGTTATCGGAGC
TGTTGCGGGCTTCGGCTTCTTATAAATCAGATGTTGAGCTGTGTATTTATAATAGTAATGGGCTGGATAATTGCTCCGGT
GGTACAAATGGTATTCAAGCCAATAAAACGAGTGGTAATGAAACCAAATATCTTAATTCGATTACCGTAACTAAAGGCAT
TATTACCGTAACAGGCAAAGGCTCGCTTGAAGGTTATAGTTATACGTTAACACCAAAATTTGCAAACAATACGATTAGTT
GGTCATCGAGTTGTAGCGGTAAAGATACCAGCCTTTTCCCTGCAGGCTTTTGTAATACCGCATCAGCTGCATCAAACGAC
TAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1C1J3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.667

95.625

0.638
  pilA Haemophilus influenzae 86-028NP

57.639

90

0.519
  pilA Haemophilus influenzae Rd KW20

55.172

90.625

0.5