Detailed information NTG0480561

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: IAR44_RS12550
• Genome accession: NZ_CP061087
• Coordinates: 2619953..2620567 (-)
• Length: 204 a.a.
• NCBI ID: WP_061860722.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CLA178
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2614953..2625567

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IAR44_RS12520 (IAR44_12520)	rpsT	2615256..2615522 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
IAR44_RS12525 (IAR44_12525)	holA	2615539..2616580 (-)	1042	Protein_2445	DNA polymerase III subunit delta	-
IAR44_RS12530 (IAR44_12530)	-	2616620..2616771 (-)	152	Protein_2446	hypothetical protein	-
IAR44_RS12535 (IAR44_12535)	-	2616812..2616946 (+)	135	WP_003152870.1	YqzM family protein	-
IAR44_RS12540 (IAR44_12540)	comEC	2616965..2619316 (-)	2352	WP_060674655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IAR44_RS12545 (IAR44_12545)	-	2619317..2619886 (-)	570	WP_003152868.1	ComE operon protein 2	-
IAR44_RS12550 (IAR44_12550)	comEA	2619953..2620567 (-)	615	WP_061860722.1	helix-hairpin-helix domain-containing protein	Machinery gene
IAR44_RS12555 (IAR44_12555)	comER	2620626..2621447 (+)	822	WP_061860723.1	late competence protein ComER	-
IAR44_RS12560 (IAR44_12560)	-	2621516..2622253 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
IAR44_RS12565 (IAR44_12565)	rsfS	2622250..2622606 (-)	357	WP_007408260.1	ribosome silencing factor	-
IAR44_RS12570 (IAR44_12570)	yqeK	2622624..2623184 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IAR44_RS12575 (IAR44_12575)	-	2623174..2623743 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
IAR44_RS12580 (IAR44_12580)	yhbY	2623754..2624044 (-)	291	WP_061860725.1	ribosome assembly RNA-binding protein YhbY	-
IAR44_RS12585 (IAR44_12585)	aroE	2624038..2624880 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22263.50 Da
• Isoelectric Point: 6.4888

>NTDB_id=480561 IAR44_RS12550 WP_061860722.1 2619953..2620567(-) (comEA) [Bacillus velezensis strain CLA178]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNINLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=480561 IAR44_RS12550 WP_061860722.1 2619953..2620567(-) (comEA) [Bacillus velezensis strain CLA178]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATAT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.143	96.078	0.549
comEA	Lactococcus lactis subsp. cremoris KW2	35.586	100	0.387
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363