Detailed information NTG0479376

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IAG34_RS16870
• Genome accession: NZ_CP060994
• Coordinates: 3428928..3429986 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain CAb-65
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3423928..3434986

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IAG34_RS16845 (IAG34_16840)	aroK	3424203..3424772 (-)	570	WP_000643712.1	shikimate kinase AroK	-
IAG34_RS16850 (IAG34_16845)	pilQ	3424784..3426949 (-)	2166	WP_004742153.1	type IV pilus secretin PilQ family protein	Machinery gene
IAG34_RS16855 (IAG34_16850)	pilP	3427012..3427539 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
IAG34_RS16860 (IAG34_16855)	pilO	3427550..3428290 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
IAG34_RS16865 (IAG34_16860)	pilN	3428287..3428928 (-)	642	WP_110989352.1	PilN domain-containing protein	Machinery gene
IAG34_RS16870 (IAG34_16865)	pilM	3428928..3429986 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
IAG34_RS16875 (IAG34_16870)	ponA	3430149..3432704 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
IAG34_RS16880 (IAG34_16875)	-	3432688..3433545 (-)	858	WP_217408552.1	putative RNA methyltransferase	-
IAG34_RS16885 (IAG34_16880)	-	3433548..3433880 (-)	333	WP_000169599.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=479376 IAG34_RS16870 WP_002014066.1 3428928..3429986(-) (pilM) [Acinetobacter baumannii strain CAb-65]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=479376 IAG34_RS16870 WP_002014066.1 3428928..3429986(-) (pilM) [Acinetobacter baumannii strain CAb-65]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423