Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H8B22_RS03690 Genome accession   NZ_CP060820
Coordinates   788174..789892 (+) Length   572 a.a.
NCBI ID   WP_187712772.1    Uniprot ID   -
Organism   Agrilutibacter terrestris strain II4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783174..794892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8B22_RS03670 (H8B22_03670) - 783684..784532 (-) 849 WP_187712768.1 hypothetical protein -
  H8B22_RS03675 (H8B22_03675) - 784546..786066 (-) 1521 WP_187712769.1 lipopolysaccharide biosynthesis protein -
  H8B22_RS03680 (H8B22_03680) gmd 786072..787166 (-) 1095 WP_187712770.1 GDP-mannose 4,6-dehydratase -
  H8B22_RS03685 (H8B22_03685) - 787156..788013 (-) 858 WP_187712771.1 glycosyltransferase family 2 protein -
  H8B22_RS03690 (H8B22_03690) pilB 788174..789892 (+) 1719 WP_187712772.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H8B22_RS03695 (H8B22_03695) pilC 789959..791206 (+) 1248 WP_187712773.1 type II secretion system F family protein Machinery gene
  H8B22_RS03700 (H8B22_03700) pilD 791227..792093 (+) 867 WP_187712774.1 A24 family peptidase Machinery gene
  H8B22_RS03705 (H8B22_03705) coaE 792126..792746 (+) 621 WP_187712775.1 dephospho-CoA kinase -
  H8B22_RS03710 (H8B22_03710) - 792752..793687 (-) 936 WP_225876270.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62288.77 Da        Isoelectric Point: 5.9641

>NTDB_id=478610 H8B22_RS03690 WP_187712772.1 788174..789892(+) (pilB) [Agrilutibacter terrestris strain II4]
MTANLVGITGIARRLVLDGALEEQPARDAMTAATAERKPMSTYLVEKRLVTQAQLAAANSIEFGVPLFDVAVLDPSQTAI
KLVSEELVRKHNVLPMFKRGNRLFVGISDPTNTVALDEIKFQSNLVVEAILVDEDKIHRTIEQWLATADALNDSMGDDEG
MENLSTSGGDDDLAAAGDSGVDAKGDDTPVVKFINKVLVDAIRKGASDIHFEPYETDYRVRLRIDGILKQVAKVPVKLQA
RIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGGAAKLGIEKLGYEADQQKLFVDA
AVKPYGMVLVTGPTGSGKTVSLYTALNILNNEERNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVIMV
GEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVTLVIAQRLARRLHDCKREMSIPEHALLA
EGFTHEEIAGGMKIYEAVGCPDCTEGYKGRTGIYQVMPMSEEIQAIVLEGGNAMKIAEAAQKSGVRDLRQSALLKVKNGV
TSLAEINRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=478610 H8B22_RS03690 WP_187712772.1 788174..789892(+) (pilB) [Agrilutibacter terrestris strain II4]
ATGACCGCCAACCTGGTGGGAATTACCGGCATTGCCCGTCGCCTCGTTCTGGATGGAGCCCTTGAGGAACAGCCCGCACG
CGATGCGATGACTGCGGCAACGGCCGAACGCAAGCCGATGTCGACTTACCTGGTCGAGAAGCGCCTGGTCACCCAAGCCC
AACTCGCCGCGGCCAACTCGATCGAGTTCGGCGTCCCCCTCTTCGACGTCGCGGTCCTAGACCCGTCGCAGACCGCGATC
AAGTTGGTCAGCGAGGAACTTGTCCGGAAGCACAACGTGCTCCCGATGTTCAAGCGGGGTAACCGCCTGTTCGTCGGCAT
CTCGGACCCGACCAACACCGTCGCGCTGGACGAGATCAAGTTCCAGAGCAACCTGGTGGTTGAAGCCATCCTTGTCGATG
AAGACAAGATCCACCGGACCATAGAGCAGTGGCTTGCGACCGCGGACGCGCTCAACGACTCGATGGGCGACGACGAGGGG
ATGGAGAACCTTTCCACCTCGGGCGGCGACGACGACTTGGCGGCCGCCGGCGACTCTGGCGTCGATGCCAAGGGCGACGA
CACGCCCGTGGTCAAGTTCATCAACAAAGTACTGGTGGACGCCATCCGCAAGGGCGCGTCGGACATCCACTTCGAACCGT
ACGAGACCGATTACCGGGTGCGCCTGCGCATCGACGGCATCCTGAAGCAGGTGGCCAAGGTTCCGGTGAAGTTGCAGGCG
CGCATCGCCGCGCGCCTGAAAGTCATGGCCCAGTTGGATATCGCCGAAAAGCGGGTGCCCCAGGACGGGCGCATCAAGCT
CAACCTGTCCAAGACCAAGCAGGTGGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGGTCGTGCTGCGTA
TCCTCGACGGCGGCGCGGCCAAGCTGGGCATCGAAAAGCTCGGTTACGAAGCCGACCAGCAGAAGCTGTTCGTCGACGCC
GCCGTCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTATCGCTGTACACCGCACTCAA
CATCCTCAATAACGAGGAGCGCAACATCTCCACCGTCGAGGACCCGGTGGAAATCCGCGTACCCGGCATCAACCAGGTGC
AGATGAATGTGAAGCGCGGCATGACCTTCGCGGCCGCGCTGCGCAGCTTCCTTCGCCAGGACCCGGACGTGATCATGGTC
GGCGAAATCCGCGACCTGGAGACTGCCGAGATCGCGGTGAAGGCGGCGCAGACCGGCCACATGGTGCTTTCCACACTGCA
TACCAACGACGCGCCCCAGACCATCGCTCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCACCTCATCGGTTACTT
TGGTCATCGCCCAGCGCCTTGCGCGGCGCTTGCACGACTGCAAGCGCGAGATGAGCATCCCCGAGCATGCGCTGCTGGCC
GAGGGCTTCACCCACGAGGAAATCGCCGGCGGCATGAAGATCTACGAAGCCGTGGGTTGCCCGGATTGCACCGAGGGCTA
CAAGGGGCGCACCGGCATTTACCAGGTCATGCCGATGTCAGAAGAAATCCAGGCCATCGTGCTCGAAGGCGGCAACGCCA
TGAAGATCGCGGAAGCCGCGCAGAAGTCGGGGGTGCGCGACCTCCGCCAGTCGGCGCTGCTGAAGGTCAAGAACGGTGTC
ACCAGCCTTGCCGAAATCAATCGTGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

99.126

0.545
  pilB Acinetobacter baylyi ADP1

54.497

99.126

0.54
  pilB Legionella pneumophila strain ERS1305867

50.877

99.65

0.507
  pilB Vibrio cholerae strain A1552

48.252

100

0.483
  pilF Neisseria gonorrhoeae MS11

47.627

99.476

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

47.94

93.357

0.448
  pilB Vibrio campbellii strain DS40M4

50.405

86.364

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.91

93.007

0.362
  pilB/pilB1 Synechocystis sp. PCC 6803

39.313

91.608

0.36