Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H8B22_RS03700 Genome accession   NZ_CP060820
Coordinates   791227..792093 (+) Length   288 a.a.
NCBI ID   WP_187712774.1    Uniprot ID   -
Organism   Agrilutibacter terrestris strain II4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 786227..797093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8B22_RS03685 (H8B22_03685) - 787156..788013 (-) 858 WP_187712771.1 glycosyltransferase family 2 protein -
  H8B22_RS03690 (H8B22_03690) pilB 788174..789892 (+) 1719 WP_187712772.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H8B22_RS03695 (H8B22_03695) pilC 789959..791206 (+) 1248 WP_187712773.1 type II secretion system F family protein Machinery gene
  H8B22_RS03700 (H8B22_03700) pilD 791227..792093 (+) 867 WP_187712774.1 A24 family peptidase Machinery gene
  H8B22_RS03705 (H8B22_03705) coaE 792126..792746 (+) 621 WP_187712775.1 dephospho-CoA kinase -
  H8B22_RS03710 (H8B22_03710) - 792752..793687 (-) 936 WP_225876270.1 Nudix family hydrolase -
  H8B22_RS03715 (H8B22_03715) secA 793856..796585 (-) 2730 WP_187712777.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31822.75 Da        Isoelectric Point: 8.7727

>NTDB_id=478612 H8B22_RS03700 WP_187712774.1 791227..792093(+) (pilD) [Agrilutibacter terrestris strain II4]
MAFLDQNPAIGYPLVAGFGLLVGSFLNVVILRLPKRLEWEWKRDARDVLEQPEVYDPPPPGIVVERSHCPHCKHQLSWYE
NIPVFSWLALSGKCRNCKAPISIQYPLVELLTMLLFVAAVWRFGFGWQGFGAIVFSGFLISMSGIDLRTQLLPDSLTLPL
MWLGLIAAADNLYMPVKPALLGAVAGYVSLWSVWWLFKQLTGKEGMGHGDFKLLAAIGAWTGLKGVLPTILISSVVGAVI
GSIWLALKGRDRSVPIPFGPYLAIAGWIVFFWGQPMIDAYLRFAGLAP

Nucleotide


Download         Length: 867 bp        

>NTDB_id=478612 H8B22_RS03700 WP_187712774.1 791227..792093(+) (pilD) [Agrilutibacter terrestris strain II4]
ATGGCATTCCTCGACCAGAACCCCGCCATCGGCTATCCGCTGGTGGCGGGCTTCGGGCTGCTGGTGGGCAGCTTCCTCAA
CGTGGTGATCCTGCGCCTGCCCAAGCGGCTGGAGTGGGAGTGGAAGCGCGATGCGCGCGACGTGCTCGAGCAGCCGGAGG
TCTACGACCCGCCGCCGCCGGGCATCGTCGTCGAGCGCTCGCACTGCCCGCACTGCAAGCACCAGCTGAGCTGGTACGAG
AACATCCCGGTGTTCAGCTGGCTGGCGCTGAGCGGCAAGTGCCGCAACTGCAAGGCGCCGATCTCGATCCAGTATCCGCT
GGTCGAATTGCTCACCATGTTGCTGTTCGTGGCGGCGGTGTGGCGCTTCGGCTTCGGCTGGCAGGGCTTCGGGGCCATCG
TCTTCAGCGGCTTCCTCATCTCGATGTCCGGCATCGACCTGCGCACGCAGTTGCTGCCCGACAGCCTGACGTTGCCGCTG
ATGTGGCTGGGCCTGATCGCGGCCGCCGACAACCTGTACATGCCGGTGAAGCCCGCCCTGCTTGGCGCGGTGGCGGGCTA
CGTCAGCCTCTGGTCGGTGTGGTGGCTGTTCAAGCAGCTCACCGGCAAGGAAGGCATGGGCCATGGCGACTTCAAGCTGC
TGGCGGCCATCGGCGCCTGGACCGGGCTGAAGGGCGTGCTGCCGACGATCCTCATCTCCTCGGTGGTCGGCGCGGTGATC
GGTTCGATCTGGCTGGCGCTCAAGGGCCGCGACCGCTCGGTGCCGATTCCGTTCGGCCCCTACCTCGCGATCGCGGGCTG
GATCGTGTTCTTCTGGGGCCAGCCGATGATCGATGCCTACCTGCGCTTCGCGGGTCTCGCACCCTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)

Type IV leader peptidase family (Peptidase_A24)

(134-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.273

95.486

0.49
  pilD Acinetobacter baumannii D1279779

46.996

98.264

0.462
  pilD Vibrio campbellii strain DS40M4

49.438

92.708

0.458
  pilD Acinetobacter nosocomialis M2

46.29

98.264

0.455
  pilD Neisseria gonorrhoeae MS11

47.104

89.931

0.424