Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H9L24_RS19965 Genome accession   NZ_CP060790
Coordinates   4199071..4200807 (-) Length   578 a.a.
NCBI ID   WP_187736097.1    Uniprot ID   A0A7H0HEZ6
Organism   Paenacidovorax monticola strain KACC 19171     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4194071..4205807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9L24_RS19935 (H9L24_19935) - 4194787..4195224 (+) 438 WP_246483502.1 NUDIX domain-containing protein -
  H9L24_RS19940 (H9L24_19940) - 4195271..4195480 (-) 210 WP_187736092.1 DNA gyrase inhibitor YacG -
  H9L24_RS19945 (H9L24_19945) zapD 4195494..4196249 (-) 756 WP_187736093.1 cell division protein ZapD -
  H9L24_RS19950 (H9L24_19950) coaE 4196326..4196934 (-) 609 WP_187736094.1 dephospho-CoA kinase -
  H9L24_RS19955 (H9L24_19955) pilD 4196937..4197815 (-) 879 WP_187736095.1 A24 family peptidase Machinery gene
  H9L24_RS19960 (H9L24_19960) pilC 4197815..4199032 (-) 1218 WP_187736096.1 type II secretion system F family protein Machinery gene
  H9L24_RS19965 (H9L24_19965) pilB 4199071..4200807 (-) 1737 WP_187736097.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H9L24_RS19975 (H9L24_19975) - 4201044..4201973 (-) 930 WP_187738411.1 polyprenyl synthetase family protein -
  H9L24_RS19980 (H9L24_19980) rplU 4202187..4202498 (+) 312 WP_187736098.1 50S ribosomal protein L21 -
  H9L24_RS19985 (H9L24_19985) rpmA 4202511..4202768 (+) 258 WP_187736099.1 50S ribosomal protein L27 -
  H9L24_RS19990 (H9L24_19990) cgtA 4202926..4203996 (+) 1071 WP_187736100.1 Obg family GTPase CgtA -
  H9L24_RS19995 (H9L24_19995) proB 4204084..4205223 (+) 1140 WP_187736101.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62882.19 Da        Isoelectric Point: 5.3439

>NTDB_id=478351 H9L24_RS19965 WP_187736097.1 4199071..4200807(-) (pilB) [Paenacidovorax monticola strain KACC 19171]
MAAVDTAQKEASAIALPGLGRALISAGKLTQKTAEDIYKKSQISRSSFIAELTGSGAVSAADLAYTVASVFGAPLLDLDA
IDPMRLPKELLDAKICQAYRVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLTKLVEATSKSVSESM
DTLTSGSEFEFDDVSVEEAPEAADTGVTEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHTYRVRFRIDGELREIASPP
IAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPQEKE
RLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDP
DIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKTPADIP
HEALVNAGYDENEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQAKLEGVRSLREAGLH
KARIGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=478351 H9L24_RS19965 WP_187736097.1 4199071..4200807(-) (pilB) [Paenacidovorax monticola strain KACC 19171]
ATGGCAGCTGTTGATACCGCACAGAAGGAAGCCTCCGCGATCGCCCTCCCTGGCCTGGGCCGGGCTTTGATATCCGCAGG
CAAGCTGACCCAGAAAACCGCCGAGGATATTTACAAAAAGTCACAAATAAGCCGTTCCAGCTTCATTGCCGAGCTGACCG
GCTCTGGCGCGGTCTCTGCCGCGGATCTGGCGTACACCGTGGCATCGGTCTTTGGCGCGCCCTTGCTGGATCTGGACGCC
ATCGACCCCATGCGGCTTCCCAAGGAGCTGCTGGATGCCAAGATCTGCCAGGCCTACCGTGTTGTCGTCCTGAGCAAGCG
GAACAACCGATTGATCGTGGCGACGGCCGACCCCACCGACCAGGAGGCGGCCGAGAAGATCAAGTTCACCACACAAATGG
GGGTGGACTGGATCATCGCGGAATACGACAAGCTCACCAAACTCGTCGAAGCCACGTCCAAGAGCGTCAGCGAATCGATG
GACACGCTGACCAGCGGCAGCGAGTTCGAGTTCGACGATGTCAGCGTCGAGGAAGCCCCCGAGGCCGCCGACACGGGCGT
CACGGAAGTCGAAGACGCGCCCATCGTCAAGTTCCTGCACAAGATGCTGCTTGACGCCTTCAACATGCGCGCGTCGGACC
TGCACTTCGAACCCTACGAACACACCTACCGCGTGCGCTTTCGCATCGACGGTGAATTGCGGGAGATCGCATCCCCTCCC
ATCGCCATCAAGGACAAGCTCGCATCGCGCATCAAGGTGATCTCGCGCCTGGACATTTCCGAGAAGCGCGTGCCCCAGGA
CGGCCGGATGAAGCTCAAGGTGGGCCCCGACCGGGTGATCGATTTCCGGGTGAGCACGCTGCCCACCCTGTTCGGCGAGA
AGATCGTGATCCGTATCCTGGACCCGAGCAGCGCCAAGCTGGGCATTGATGCGCTGGGCTATGAGCCGCAGGAGAAGGAG
CGCCTGCTCCATGCCATCGGCCGCCCCTACGGCATGATTCTCGTCACGGGGCCGACGGGTTCCGGCAAGACCGTGTCGCT
CTACACCTGCCTGAACCTGCTGAACAAGGCCGGGGTGAACATCGCCACGGCGGAAGATCCGTCGGAAATCAACCTGCCCG
GCGTGAACCAGGTCAACGTCAACGAGAAGGCCGGACTGACCTTCGCGACCGCGCTCAAATCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTGGGCGAAATCCGGGACCTGGAAACAGCGGACATTGCCATCAAGGCCGCCCAAACCGGCCACTTGGT
GCTGTCGACCCTGCACACCAACGATGCACCGACCACGCTGACGCGCATGCGCAACATGGGCATCGCGCCCTTCAACATCG
CCTCCAGCGTGATCCTCATCACGGCACAGCGCCTGGCCCGGCGCCTGTGCCCCGCGTGCAAGACGCCCGCCGACATTCCG
CACGAAGCGCTGGTCAATGCGGGCTACGACGAGAACGAAATCGACGGCAGCTGGGTCACCTACCGCCCCGTGGGCTGCTC
GGCCTGCAACAATGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCCGAGGAAATCCAGCGCATCATCC
TGCGCGATGGAAGCGCCCTGGAGATCGCCGAGCAGGCCAAGCTGGAAGGCGTGCGCTCGCTGCGCGAAGCCGGGCTGCAC
AAGGCCAGGATCGGTCTGACCTCTCTGGAAGAGGTGCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H0HEZ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.427

98.443

0.526
  pilB Acinetobacter baumannii D1279779

52.144

100

0.526
  pilF Neisseria gonorrhoeae MS11

53.097

97.751

0.519
  pilB Legionella pneumophila strain ERS1305867

48.759

97.578

0.476
  pilB Vibrio campbellii strain DS40M4

45.07

98.27

0.443
  pilB Vibrio parahaemolyticus RIMD 2210633

44.956

97.751

0.439
  pilB Vibrio cholerae strain A1552

47.601

90.138

0.429
  pilF Thermus thermophilus HB27

39.115

97.751

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.693

97.924

0.379