Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H9K76_RS04750 Genome accession   NZ_CP060714
Coordinates   1049100..1050836 (-) Length   578 a.a.
NCBI ID   WP_187598419.1    Uniprot ID   A0A7G9RRF0
Organism   Diaphorobacter ruginosibacter strain DSM 27467     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1044100..1055836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K76_RS04720 (H9K76_04720) mutT 1044732..1045169 (+) 438 WP_187598413.1 8-oxo-dGTP diphosphatase MutT -
  H9K76_RS04725 (H9K76_04725) - 1045198..1045449 (-) 252 WP_187598414.1 DNA gyrase inhibitor YacG -
  H9K76_RS04730 (H9K76_04730) zapD 1045505..1046260 (-) 756 WP_187598415.1 cell division protein ZapD -
  H9K76_RS04735 (H9K76_04735) coaE 1046331..1046963 (-) 633 WP_187598416.1 dephospho-CoA kinase -
  H9K76_RS04740 (H9K76_04740) pilD 1046982..1047857 (-) 876 WP_187598417.1 A24 family peptidase Machinery gene
  H9K76_RS04745 (H9K76_04745) pilC 1047857..1049074 (-) 1218 WP_187598418.1 type II secretion system F family protein Machinery gene
  H9K76_RS04750 (H9K76_04750) pilB 1049100..1050836 (-) 1737 WP_187598419.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H9K76_RS04755 (H9K76_04755) - 1051250..1052221 (-) 972 WP_187598420.1 tripartite tricarboxylate transporter substrate binding protein -
  H9K76_RS04760 (H9K76_04760) - 1052470..1053174 (-) 705 WP_187598421.1 metallophosphoesterase -
  H9K76_RS04770 (H9K76_04770) - 1053422..1054351 (-) 930 WP_187598422.1 polyprenyl synthetase family protein -
  H9K76_RS04775 (H9K76_04775) rplU 1054614..1054925 (+) 312 WP_187600458.1 50S ribosomal protein L21 -
  H9K76_RS04780 (H9K76_04780) rpmA 1054938..1055195 (+) 258 WP_187598423.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62888.22 Da        Isoelectric Point: 5.1651

>NTDB_id=477974 H9K76_RS04750 WP_187598419.1 1049100..1050836(-) (pilB) [Diaphorobacter ruginosibacter strain DSM 27467]
MAAVDTAPKSATPIVLPGLARALMSAGKLDQNAAEELVKKAQGARSTFIAELINGGTITSADLAHTISSVFGAPLIDVDA
LDTIRIPSDLLDAKICQAYRVAVLNKRGNRLIIATADPTDHEAAEKIKFTTQMGVDWIIAEYDKLGRFVEKVTKGATEAL
DSIVSGGDFEFGDVPVEETADAQDVGQNDVEDAPIVKFLQKMLIDAFNMRASDLHFEPYEHNYRVRFRVDGELREISSPP
PAIKEKLASRIKVISRLDISEKRVPQDGRMKLKIGPERVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEPEEKE
RLLNAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQDP
DIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCGTCKEPADIP
HEALVEAGYREEEIDGSWVTYKPVGCNACNNGYKGRVGIYEVMPISEEMQRIILQDGSALEIAKQARLEGVRSLRTSGLY
KAKIGMTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=477974 H9K76_RS04750 WP_187598419.1 1049100..1050836(-) (pilB) [Diaphorobacter ruginosibacter strain DSM 27467]
ATGGCCGCTGTAGACACTGCCCCCAAAAGCGCGACTCCCATTGTTCTGCCCGGATTGGCCAGGGCACTGATGTCCGCCGG
CAAGCTCGACCAGAATGCCGCGGAAGAACTGGTAAAGAAGGCGCAGGGTGCACGCAGCACATTCATTGCAGAGCTGATCA
ACGGCGGCACCATCACGTCGGCGGATCTCGCCCACACTATCTCCAGCGTCTTCGGCGCGCCGCTGATCGACGTGGATGCG
CTCGATACGATACGCATCCCCAGCGATCTTCTGGATGCGAAGATCTGCCAGGCCTATCGTGTGGCCGTGCTGAACAAGCG
CGGAAATCGTCTCATCATTGCAACAGCGGACCCAACCGATCACGAAGCCGCGGAGAAGATCAAGTTCACCACCCAGATGG
GCGTGGACTGGATCATCGCGGAGTACGACAAGCTGGGCCGCTTCGTCGAAAAGGTGACGAAGGGTGCGACAGAGGCGCTC
GACTCCATCGTCTCCGGTGGTGACTTTGAATTCGGCGACGTCCCGGTGGAGGAGACGGCGGATGCGCAGGACGTGGGCCA
GAACGATGTGGAAGACGCTCCCATCGTCAAGTTCCTGCAGAAGATGCTGATCGATGCGTTCAACATGCGCGCATCCGACC
TTCACTTCGAGCCCTACGAACACAACTACCGCGTGCGCTTTCGCGTCGACGGCGAGCTGCGCGAGATTTCCAGCCCCCCG
CCTGCGATCAAGGAAAAGCTCGCATCGCGCATCAAGGTGATCTCGCGCCTCGATATTTCCGAAAAGCGCGTTCCCCAGGA
CGGGCGGATGAAGCTCAAGATCGGCCCCGAGCGCGTGATCGATTTCCGTGTGAGCACCCTGCCCACGCTGTTCGGCGAAA
AGATCGTGATCCGTATTCTCGACCCCAGCAGCGCCAAGCTGGGCATCGAGGCACTCGGCTACGAGCCCGAGGAGAAAGAG
CGCCTGCTCAACGCCATCGGCCGTCCCTATGGAATGATCCTGGTGACAGGCCCCACCGGCTCGGGCAAGACGGTGTCGCT
CTACACCTGCCTGAACCTGCTCAACAAGCCCGGGGTGAACATCTCCACGGCCGAGGACCCATCCGAAATCAACTTGCCTG
GCGTGAACCAGGTCAACGTGAACGAAAAGGCGGGCCTGACCTTCGCGACCGCACTCAAGGCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGTGAAATTCGCGACCTGGAAACCGCCGACATTGCCATCAAGGCGGCCCAGACGGGTCACCTGGT
GCTGTCTACCTTGCACACCAACGATGCGCCCACGACACTGACGCGTATGCGCAACATGGGGATCGCCCCGTTCAACATTG
CCTCGAGCGTGATCCTGATCACGGCCCAGCGCTTGGCACGCCGCCTGTGCGGCACCTGCAAGGAGCCGGCGGACATCCCG
CACGAGGCCCTGGTCGAGGCCGGCTATCGCGAAGAGGAAATCGACGGCTCGTGGGTGACTTACAAGCCGGTGGGCTGCAA
TGCCTGCAACAATGGCTACAAGGGCCGGGTCGGCATCTACGAGGTGATGCCGATTTCCGAGGAGATGCAGCGCATCATCC
TGCAGGACGGCAGCGCGCTCGAGATCGCCAAGCAGGCGCGCCTGGAAGGAGTTCGCTCACTGCGCACATCCGGCCTGTAC
AAGGCCAAGATCGGCATGACTTCGCTGGAAGAAGTCCTGGCCGTCACGAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G9RRF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.266

100

0.55
  pilB Acinetobacter baumannii D1279779

54.11

100

0.547
  pilF Neisseria gonorrhoeae MS11

51.773

97.578

0.505
  pilB Legionella pneumophila strain ERS1305867

49.291

97.578

0.481
  pilB Vibrio cholerae strain A1552

45.15

98.097

0.443
  pilB Vibrio campbellii strain DS40M4

44.815

98.443

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

44.602

97.751

0.436
  pilF Thermus thermophilus HB27

39.229

98.789

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.075

92.388

0.37