Detailed information NTG0477972

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H9K76_RS04740
• Genome accession: NZ_CP060714
• Coordinates: 1046982..1047857 (-)
• Length: 291 a.a.
• NCBI ID: WP_187598417.1
• Uniprot ID: A0A7G9RRE8
• Organism: Diaphorobacter ruginosibacter strain DSM 27467
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1041982..1052857

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9K76_RS04710 (H9K76_04710)	argJ	1042551..1043780 (+)	1230	WP_187598411.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
H9K76_RS04715 (H9K76_04715)	-	1043864..1044739 (+)	876	WP_187598412.1	ATP-binding protein	-
H9K76_RS04720 (H9K76_04720)	mutT	1044732..1045169 (+)	438	WP_187598413.1	8-oxo-dGTP diphosphatase MutT	-
H9K76_RS04725 (H9K76_04725)	-	1045198..1045449 (-)	252	WP_187598414.1	DNA gyrase inhibitor YacG	-
H9K76_RS04730 (H9K76_04730)	zapD	1045505..1046260 (-)	756	WP_187598415.1	cell division protein ZapD	-
H9K76_RS04735 (H9K76_04735)	coaE	1046331..1046963 (-)	633	WP_187598416.1	dephospho-CoA kinase	-
H9K76_RS04740 (H9K76_04740)	pilD	1046982..1047857 (-)	876	WP_187598417.1	A24 family peptidase	Machinery gene
H9K76_RS04745 (H9K76_04745)	pilC	1047857..1049074 (-)	1218	WP_187598418.1	type II secretion system F family protein	Machinery gene
H9K76_RS04750 (H9K76_04750)	pilB	1049100..1050836 (-)	1737	WP_187598419.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H9K76_RS04755 (H9K76_04755)	-	1051250..1052221 (-)	972	WP_187598420.1	tripartite tricarboxylate transporter substrate binding protein	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 31675.42 Da
• Isoelectric Point: 6.9224

>NTDB_id=477972 H9K76_RS04740 WP_187598417.1 1046982..1047857(-) (pilD) [Diaphorobacter ruginosibacter strain DSM 27467]
MTGEPWLDAVVVGILGLLIGSFLNVVIHRVPIMMEREWEQECRDYIEDKGWPAPPPSGHTGRFSLLAPASRCPHCGHAIR
WYENIPVISYVALRGKCAGCKARISIRYPLVELITGGLFYWCATQWGWSMTTALWCAFSAALVSLTMIDWDTTLLPDSIT
LPLLWAGLIGSAAHWLSVPLVDALFGAAAGYLFLWSIYWAFKLLTGKEGMGYGDFKLLAALGAWFGWSALVPIILIASVI
GAIVGIGMKLTSSLREGQYVPFGPFLAGAGFAAMLLGPERILQSVLSLLGI

Nucleotide

• Length: 876 bp

>NTDB_id=477972 H9K76_RS04740 WP_187598417.1 1046982..1047857(-) (pilD) [Diaphorobacter ruginosibacter strain DSM 27467]
ATGACCGGTGAGCCATGGCTGGATGCCGTCGTCGTCGGCATCCTCGGACTTCTGATCGGCAGTTTTCTCAACGTCGTGAT
CCACCGCGTCCCCATCATGATGGAGCGTGAATGGGAACAGGAGTGCCGCGACTACATCGAGGACAAGGGCTGGCCCGCGC
CGCCCCCTTCCGGACACACGGGCCGTTTCAGCCTGCTGGCGCCCGCATCGCGCTGCCCGCACTGCGGCCACGCGATCCGC
TGGTACGAGAACATTCCCGTCATCAGCTACGTCGCGTTGCGCGGCAAATGTGCGGGCTGCAAGGCACGCATCAGCATCCG
CTACCCGCTGGTGGAACTAATCACCGGTGGCCTCTTCTACTGGTGCGCCACCCAATGGGGCTGGTCGATGACCACGGCCC
TGTGGTGCGCCTTCTCGGCGGCCCTCGTGTCGCTGACCATGATCGACTGGGATACGACGCTGCTGCCCGACAGCATCACG
CTGCCCCTGTTGTGGGCCGGCCTGATCGGCTCCGCCGCGCACTGGCTCAGCGTGCCCCTCGTCGATGCCCTGTTCGGGGC
TGCGGCGGGCTACCTGTTCCTCTGGTCGATCTACTGGGCGTTCAAGCTGCTCACCGGCAAGGAAGGCATGGGCTACGGCG
ATTTCAAGCTGCTCGCGGCGCTGGGTGCCTGGTTCGGATGGTCGGCGCTGGTGCCCATCATCCTGATCGCCTCGGTGATC
GGCGCGATCGTGGGAATCGGCATGAAGCTCACCAGCTCACTGCGTGAAGGCCAGTACGTACCGTTCGGCCCCTTCCTGGC
AGGCGCGGGGTTTGCAGCCATGCTTCTCGGCCCGGAGAGAATCCTGCAATCCGTGCTTTCGCTGCTGGGTATCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-125)

3D structure

• AlphaFold DB: A0A7G9RRE8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	48.611	98.969	0.481
pilD	Vibrio cholerae strain A1552	50.181	95.189	0.478
pilD	Neisseria gonorrhoeae MS11	50	88.66	0.443
pilD	Acinetobacter baumannii D1279779	46.296	92.784	0.43
pilD	Acinetobacter nosocomialis M2	45.556	92.784	0.423