Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H9L41_RS02430 Genome accession   NZ_CP060704
Coordinates   576159..577871 (-) Length   570 a.a.
NCBI ID   WP_028446056.1    Uniprot ID   -
Organism   Chitinimonas koreensis strain R2A43-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 571159..582871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9L41_RS02400 (H9L41_02400) - 571545..572300 (-) 756 WP_028446062.1 GNAT family N-acetyltransferase -
  H9L41_RS02405 (H9L41_02405) - 572401..572586 (-) 186 WP_028446061.1 DNA gyrase inhibitor YacG -
  H9L41_RS02410 (H9L41_02410) zapD 572586..573344 (-) 759 WP_028446060.1 cell division protein ZapD -
  H9L41_RS02415 (H9L41_02415) coaE 573416..574036 (-) 621 WP_028446059.1 dephospho-CoA kinase -
  H9L41_RS02420 (H9L41_02420) pilD 574039..574905 (-) 867 WP_034606803.1 A24 family peptidase Machinery gene
  H9L41_RS02425 (H9L41_02425) pilC 574912..576135 (-) 1224 WP_028446057.1 type II secretion system F family protein Machinery gene
  H9L41_RS02430 (H9L41_02430) pilB 576159..577871 (-) 1713 WP_028446056.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H9L41_RS02435 (H9L41_02435) - 578057..578887 (-) 831 WP_051318970.1 inner membrane protein YpjD -
  H9L41_RS02440 (H9L41_02440) ffh 578973..580321 (+) 1349 Protein_498 signal recognition particle protein -
  H9L41_RS02445 (H9L41_02445) rpsP 580444..580686 (+) 243 WP_028446053.1 30S ribosomal protein S16 -
  H9L41_RS02450 (H9L41_02450) rimM 580770..581252 (+) 483 WP_028446052.1 ribosome maturation factor RimM -
  H9L41_RS02455 (H9L41_02455) trmD 581245..582027 (+) 783 WP_373278947.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  H9L41_RS02460 (H9L41_02460) rplS 582071..582454 (+) 384 WP_028446050.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62145.51 Da        Isoelectric Point: 5.0241

>NTDB_id=477849 H9L41_RS02430 WP_028446056.1 576159..577871(-) (pilB) [Chitinimonas koreensis strain R2A43-10]
MAQSPPSLSGIGRALIQQGRLLEADVEAIQSQAHSSNTGFVQALIASKKMNGLQIAEFASSTFGYPLFDLAQFDSAYIPA
GALDPKLMQTRRIAPLYKRGTKLFIAISDPTNMQALEDAKFQTSAMIEPIVVEDQKLGALIDKLVEATGANLKNLVIDQE
DLEMAGGDDIQTEQEGPSIEVDDAPVVKYIQKMLMDAINSGASDIHFEPYEKFYRIRYRIDGVLREIAQPPLAIKEKIAS
RIKVISKLDISEKRIPQDGRMKLVLSKNRAIDFRVSTLPTLFGEKICMRILDPSSATLGIDALGYEPDQRAALLDAIHRP
YGMVLVTGPTGSGKTVSLYTCLNILNEPGINISTAEDPAEINLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEIR
DLETADIAIKAAQTGHMVFSTLHTNDAPATLSRMLNMGIAPFNIASSVILITGQRLARRLCSACKKPVDIPTEALLQAGF
KEEDLDGSWQPYGPVGCDICKGSGYKGRLGIYQVMPISEDMQRIIMQQGNSIDIADQARREGIRDLRQSGLLKVKQGLTS
LTEVEAVTNE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=477849 H9L41_RS02430 WP_028446056.1 576159..577871(-) (pilB) [Chitinimonas koreensis strain R2A43-10]
ATGGCGCAATCCCCGCCCTCCCTCTCCGGCATCGGCCGTGCACTGATCCAGCAAGGCCGGCTGCTGGAAGCAGACGTCGA
AGCGATCCAGTCGCAGGCCCACAGCAGCAACACCGGTTTCGTCCAGGCGCTGATCGCCAGCAAGAAGATGAACGGCCTGC
AGATCGCCGAGTTCGCCTCCAGCACCTTCGGCTATCCGCTGTTCGACCTCGCCCAGTTCGACTCGGCCTACATCCCGGCC
GGCGCGCTCGACCCCAAGCTGATGCAGACGCGGCGGATCGCGCCGCTGTACAAGCGCGGCACCAAGCTGTTCATCGCCAT
TTCCGACCCCACCAACATGCAGGCGCTGGAAGACGCGAAGTTCCAGACCAGCGCCATGATCGAGCCGATCGTGGTGGAGG
ACCAGAAGCTCGGCGCGCTGATCGACAAGCTGGTCGAAGCCACCGGCGCCAACCTGAAGAACCTCGTCATCGACCAGGAA
GACCTGGAGATGGCCGGCGGCGACGACATCCAGACCGAGCAGGAAGGCCCGTCGATCGAAGTCGACGACGCGCCGGTGGT
GAAGTACATCCAGAAGATGCTGATGGATGCGATCAACAGCGGCGCCTCGGACATCCATTTCGAGCCGTACGAGAAGTTCT
ACCGCATCCGTTACCGCATCGACGGCGTGCTGCGCGAAATCGCCCAGCCGCCGCTGGCCATCAAGGAAAAGATCGCCTCC
CGGATCAAGGTGATCTCCAAGCTCGACATCTCGGAAAAGCGCATCCCGCAGGATGGCCGGATGAAGCTGGTGCTGTCCAA
GAACCGCGCGATCGATTTCCGGGTCTCGACGCTGCCGACGCTGTTCGGCGAAAAGATCTGTATGCGGATTCTCGATCCGA
GCTCCGCCACGCTCGGCATCGACGCGCTCGGCTACGAGCCCGACCAGCGCGCCGCGCTGCTCGACGCGATCCACCGGCCC
TACGGTATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCTGCCTCAACATCCTCAACGA
GCCCGGCATCAATATCTCGACCGCGGAAGACCCGGCCGAAATCAACCTGCCCGGCATCAACCAGGTCAATGTCAACGACA
AGGCCGGCCTGACCTTCGCCGCCGCGCTCAAGTCCTTCCTGCGGCAGGATCCCGACATCATCATGGTCGGTGAAATCCGC
GACCTGGAAACCGCCGACATCGCGATCAAGGCCGCCCAGACCGGCCACATGGTGTTCTCCACGCTGCACACCAACGACGC
CCCCGCCACGCTGTCGCGGATGCTGAACATGGGCATCGCGCCGTTCAACATCGCCTCCTCGGTGATCCTGATCACCGGCC
AGCGGCTGGCCCGCCGGCTGTGCAGCGCCTGCAAGAAGCCGGTCGACATCCCGACCGAGGCCCTGCTGCAAGCCGGCTTC
AAGGAAGAAGACCTGGACGGCAGCTGGCAGCCCTACGGCCCGGTCGGCTGCGACATCTGCAAGGGCAGCGGCTACAAGGG
CCGGCTCGGCATCTACCAGGTCATGCCGATCTCCGAAGACATGCAGCGCATCATCATGCAACAGGGCAATTCCATCGACA
TCGCGGACCAGGCGCGCCGCGAAGGCATCCGTGACCTCCGTCAGTCGGGGCTGTTGAAAGTCAAACAAGGGCTCACTTCG
CTGACCGAAGTCGAAGCCGTAACGAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-147)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.692

100

0.558
  pilB Acinetobacter baumannii D1279779

54.912

100

0.549
  pilF Neisseria gonorrhoeae MS11

59.846

90.877

0.544
  pilB Legionella pneumophila strain ERS1305867

52.174

100

0.526
  pilB Vibrio cholerae strain A1552

48.148

99.474

0.479
  pilB Vibrio campbellii strain DS40M4

47.34

98.947

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

46.809

98.947

0.463
  pilF Thermus thermophilus HB27

37.805

100

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.943

92.982

0.381