Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J1G50_RS03090 Genome accession   NZ_CP071489
Coordinates   567630..568094 (-) Length   154 a.a.
NCBI ID   WP_160405122.1    Uniprot ID   -
Organism   Glaesserella parasuis strain YHP1815     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 562630..573094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G50_RS03070 (J1G50_03070) dapE 563127..564260 (-) 1134 WP_160405114.1 succinyl-diaminopimelate desuccinylase -
  J1G50_RS03075 (J1G50_03075) - 564344..565012 (-) 669 WP_160405116.1 prepilin peptidase -
  J1G50_RS03080 (J1G50_03080) pilC 565009..566205 (-) 1197 WP_160405118.1 type II secretion system F family protein Machinery gene
  J1G50_RS03085 (J1G50_03085) pilB 566198..567583 (-) 1386 WP_160405120.1 GspE/PulE family protein Machinery gene
  J1G50_RS03090 (J1G50_03090) pilA 567630..568094 (-) 465 WP_160405122.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J1G50_RS03095 (J1G50_03095) radA/sms 568282..569658 (+) 1377 WP_005711784.1 DNA repair protein RadA Machinery gene
  J1G50_RS03100 (J1G50_03100) gmk 569711..570337 (-) 627 WP_071610761.1 guanylate kinase -
  J1G50_RS03105 (J1G50_03105) gpt 570575..571054 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  J1G50_RS03110 (J1G50_03110) - 571234..572688 (+) 1455 WP_062924312.1 aminoacyl-histidine dipeptidase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16361.96 Da        Isoelectric Point: 9.0759

>NTDB_id=477206 J1G50_RS03090 WP_160405122.1 567630..568094(-) (pilA) [Glaesserella parasuis strain YHP1815]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKIAECSSGKN
GVQADKTDVADAKYLKSISVAAGVITVAGKGSIDGYSYTMTPKFANNNITWTTSCQGTDTSLFPANFCGTKATQ

Nucleotide


Download         Length: 465 bp        

>NTDB_id=477206 J1G50_RS03090 WP_160405122.1 567630..568094(-) (pilA) [Glaesserella parasuis strain YHP1815]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTGATGATCGTGATCGCCATCATTGC
TATTTTAGCCACGATTGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCTGCACTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCGCAGAATGTAGTTCTGGTAAAAAT
GGCGTTCAAGCGGATAAAACCGATGTTGCCGATGCGAAATATCTTAAATCTATTTCCGTCGCTGCAGGTGTAATCACAGT
GGCAGGTAAAGGCAGTATTGATGGTTACAGCTACACAATGACTCCGAAATTTGCCAATAACAACATTACTTGGACAACAA
GCTGTCAAGGGACTGACACCAGTTTGTTCCCTGCGAATTTCTGTGGGACAAAAGCCACACAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

92.81

99.351

0.922
  pilA Haemophilus influenzae Rd KW20

55.333

97.403

0.539
  pilA Haemophilus influenzae 86-028NP

55.034

96.753

0.532