NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	HUG14_RS01095	Genome accession	NZ_CP060580
Coordinates	234094..234741 (-)	Length	215 a.a.
NCBI ID	WP_033899944.1	Uniprot ID	A0A2Z3KPW2
Organism	Lactococcus lactis strain K_LL005
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 229094..239741

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUG14_RS01070 (HUG14_01070)	atpF	229164..229670 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
HUG14_RS01075 (HUG14_01075)	atpB	229685..230398 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
HUG14_RS01080 (HUG14_01080)	-	230443..230658 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
HUG14_RS01085 (HUG14_01085)	-	230843..231619 (-)	777	WP_058206315.1	alpha/beta hydrolase family protein	-
HUG14_RS01090 (HUG14_01090)	comEC	231903..234113 (-)	2211	WP_187398453.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HUG14_RS01095 (HUG14_01095)	comEA	234094..234741 (-)	648	WP_033899944.1	ComEA family DNA-binding protein	Machinery gene
HUG14_RS01100 (HUG14_01100)	-	234801..236162 (-)	1362	WP_029344292.1	ABC transporter permease	-
HUG14_RS01105 (HUG14_01105)	-	236159..237091 (-)	933	WP_187398454.1	ABC transporter ATP-binding protein	-
HUG14_RS01110 (HUG14_01110)	-	237196..237594 (-)	399	WP_004255236.1	hypothetical protein	-
HUG14_RS01115 (HUG14_01115)	-	237698..238261 (-)	564	WP_129881050.1	GNAT family N-acetyltransferase	-
HUG14_RS01120 (HUG14_01120)	-	238434..239612 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

Download Length: 215 a.a. Molecular weight: 23622.79 Da Isoelectric Point: 4.8659

                                >NTDB_id=476631 HUG14_RS01095 WP_033899944.1 234094..234741(-) (comEA) [Lactococcus lactis strain K_LL005]

MDKILEKVKEYWKMIVLVVCGLIAGGVFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP

NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD

LIELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

Download Length: 648 bp

                                >NTDB_id=476631 HUG14_RS01095 WP_033899944.1 234094..234741(-) (comEA) [Lactococcus lactis strain K_LL005]

ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGGT

TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT

CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT

AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA

GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG

AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT

TTGATTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA

TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT

TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A2Z3KPW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381

Detailed information NTG0476631