Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H8F01_RS14780 Genome accession   NZ_CP060412
Coordinates   3375367..3377088 (-) Length   573 a.a.
NCBI ID   WP_187055848.1    Uniprot ID   A0A7G8Q0R1
Organism   Dyella telluris strain G9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3370367..3382088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8F01_RS14755 (H8F01_14755) - 3370925..3371413 (+) 489 WP_187055843.1 dihydrofolate reductase -
  H8F01_RS14760 (H8F01_14760) - 3371451..3372380 (+) 930 WP_187055844.1 Nudix family hydrolase -
  H8F01_RS14765 (H8F01_14765) coaE 3372402..3373025 (-) 624 WP_238480998.1 dephospho-CoA kinase -
  H8F01_RS14770 (H8F01_14770) pilD 3373022..3373879 (-) 858 WP_187055846.1 A24 family peptidase Machinery gene
  H8F01_RS14775 (H8F01_14775) pilC 3373946..3375208 (-) 1263 WP_187055847.1 type II secretion system F family protein Machinery gene
  H8F01_RS14780 (H8F01_14780) pilB 3375367..3377088 (-) 1722 WP_187055848.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H8F01_RS14785 (H8F01_14785) - 3377320..3378492 (+) 1173 WP_187055849.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  H8F01_RS14790 (H8F01_14790) - 3378614..3379468 (+) 855 WP_187055850.1 glycosyltransferase family 2 protein -
  H8F01_RS14795 (H8F01_14795) - 3379461..3380615 (+) 1155 WP_187055851.1 glycosyltransferase family 4 protein -
  H8F01_RS14800 (H8F01_14800) - 3380948..3381985 (+) 1038 WP_187055852.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62686.22 Da        Isoelectric Point: 6.2647

>NTDB_id=475792 H8F01_RS14780 WP_187055848.1 3375367..3377088(-) (pilB) [Dyella telluris strain G9]
MSTLMQPSLAGLTGLARRLVSEGVLPEADVRRAVAESTQSKTSLTAWLLDNNQVESARLTRIASDEFGMPMMDVTALVAA
NMPVNLISEALINKHHALPLFKRGKRLFVGIADPMQSHALDEIKFHSNCMVEPILVERSQLMRIIETALNAMSNAVPDMG
GGDFDELSLESGDDEAESTGYDATGNDDAPVVKFVNKILVDAIKRGASDIHFEPFESQYRVRLRMDGMLRAVAYPPMKLA
SRISSRIKVMSGLDIAERRVPQDGRIKLNLSKSRSVDFRVSTLPTLFGEKIVLRILDGSAAKLGIDALGYEDVQKQLYLE
AIHKPYGMVLVTGPTGSGKTVSLYTGLNILNTAERNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDVIM
VGEIRDLETAEIAVKAAQTGHMVLSTLHTNDASQTISRLMNMGIAPYNITSSVTLIIAQRLARRLHDCKRPMNLPPQTLL
EAGFTQEDIDAGLTLYEAVGCDNCNEGYKGRVGIYQVMPMLEEIQKIILQGGNTLQIAEAATRAGVNDLRASALLKVKKG
VTSLAEIDRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=475792 H8F01_RS14780 WP_187055848.1 3375367..3377088(-) (pilB) [Dyella telluris strain G9]
ATGTCAACGCTAATGCAACCCTCGCTCGCGGGCCTTACCGGCCTTGCGCGCAGGCTGGTATCGGAAGGTGTACTGCCCGA
GGCGGATGTCCGCCGGGCGGTGGCCGAGTCCACGCAGAGCAAAACGTCGCTCACTGCCTGGCTGCTGGACAACAACCAGG
TGGAAAGCGCCAGGCTCACCCGCATCGCGTCCGACGAGTTCGGCATGCCGATGATGGATGTCACCGCGTTGGTGGCCGCC
AACATGCCGGTGAACCTGATCAGCGAAGCGCTGATCAACAAGCACCACGCGCTGCCGCTGTTCAAGCGCGGCAAGCGACT
GTTCGTCGGCATCGCCGACCCCATGCAGTCACACGCGCTGGACGAGATCAAGTTCCACTCCAACTGCATGGTGGAGCCGA
TCCTGGTCGAGCGCAGCCAGTTGATGCGCATCATCGAAACCGCGCTCAACGCGATGAGCAACGCCGTACCCGACATGGGT
GGCGGCGATTTCGACGAGCTCTCGCTGGAAAGCGGCGACGACGAGGCCGAATCCACCGGCTACGACGCCACCGGCAACGA
CGATGCCCCGGTGGTCAAGTTCGTCAACAAGATCCTGGTCGACGCGATCAAGCGCGGCGCCTCCGATATCCATTTCGAGC
CCTTCGAATCCCAGTACCGCGTGCGCCTGCGCATGGACGGCATGCTGCGCGCCGTGGCCTACCCGCCGATGAAGCTCGCC
TCGCGCATTTCCTCGCGCATCAAGGTCATGTCTGGCCTGGACATCGCCGAACGACGTGTGCCGCAGGACGGCCGCATCAA
GCTCAACCTGTCCAAGAGCCGCTCGGTCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGCTGC
GTATCCTCGATGGCTCGGCCGCCAAGCTCGGCATCGATGCGCTGGGCTACGAGGACGTGCAAAAACAGCTCTACCTCGAA
GCCATCCACAAGCCGTACGGCATGGTCTTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACACGGGGCT
CAACATCCTCAACACCGCCGAACGCAACATCTCGACGGTGGAAGACCCGGTGGAAATCCGCGTCGAAGGCATCAACCAGG
TGCAGCAGAACGTCAAGCGCGGCATGACCTTCGCGGCGGCCCTGCGCTCCTTCCTGCGCCAGGATCCGGACGTGATCATG
GTGGGCGAAATCCGCGACCTGGAAACCGCGGAAATCGCCGTGAAGGCCGCGCAGACCGGTCACATGGTGCTGTCCACCCT
GCATACCAACGATGCTTCGCAGACGATCTCGCGACTGATGAACATGGGCATCGCGCCCTACAACATCACCTCGTCGGTCA
CCCTGATCATCGCCCAGCGTCTGGCGCGTCGCCTGCACGACTGCAAGCGTCCCATGAACCTGCCGCCGCAGACGCTGCTG
GAAGCAGGCTTCACCCAGGAAGACATCGACGCCGGCCTGACCCTCTACGAGGCCGTAGGTTGCGACAACTGCAATGAAGG
CTACAAGGGCCGCGTGGGCATCTATCAGGTCATGCCGATGCTGGAGGAGATCCAGAAGATCATCCTCCAGGGTGGCAACA
CCTTGCAGATCGCCGAAGCGGCGACCCGGGCCGGCGTAAACGACCTGCGTGCCTCGGCCTTGCTCAAGGTCAAGAAGGGC
GTGACCAGCCTGGCCGAAATCGACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8Q0R1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.261

100

0.545
  pilB Acinetobacter baumannii D1279779

54.61

98.429

0.538
  pilB Legionella pneumophila strain ERS1305867

52.724

99.302

0.524
  pilF Neisseria gonorrhoeae MS11

48.763

98.778

0.482
  pilB Vibrio cholerae strain A1552

50.551

94.939

0.48
  pilB Vibrio campbellii strain DS40M4

46.831

99.127

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

46.903

98.604

0.462
  pilF Thermus thermophilus HB27

36.667

99.476

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.278

95.288

0.365