Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7696_RS02990 Genome accession   NZ_CP060386
Coordinates   621210..622895 (-) Length   561 a.a.
NCBI ID   WP_132936924.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Vb1833     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 616210..627895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7696_RS02965 (H7696_02965) yacG 617293..617487 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  H7696_RS02970 (H7696_02970) zapD 617646..618386 (-) 741 WP_017820392.1 cell division protein ZapD -
  H7696_RS02975 (H7696_02975) coaE 618414..619028 (-) 615 WP_186584665.1 dephospho-CoA kinase -
  H7696_RS02980 (H7696_02980) pilD 619029..619898 (-) 870 WP_017635919.1 A24 family peptidase Machinery gene
  H7696_RS02985 (H7696_02985) pilC 619963..621186 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  H7696_RS02990 (H7696_02990) pilB 621210..622895 (-) 1686 WP_132936924.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7696_RS02995 (H7696_02995) - 622898..623383 (-) 486 WP_025768185.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H7696_RS03000 (H7696_03000) nadC 623646..624533 (-) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H7696_RS03005 (H7696_03005) ampD 624626..625177 (+) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H7696_RS03010 (H7696_03010) pdhR 625583..626350 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62061.95 Da        Isoelectric Point: 5.8428

>NTDB_id=475572 H7696_RS02990 WP_132936924.1 621210..622895(-) (pilB) [Vibrio alginolyticus strain Vb1833]
MLTNLSTVLRQAGILSFSQEESLLEQAKASGISMPEALLSCGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLTLAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSKQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=475572 H7696_RS02990 WP_132936924.1 621210..622895(-) (pilB) [Vibrio alginolyticus strain Vb1833]
ATGCTAACCAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGTCTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
TAAAGCTTCTGGCATTTCAATGCCGGAGGCTTTGCTCAGTTGTGGCTTTTTCCAATCACATGAGCTGACTGAGCACCTTA
GCTCTATCTTTGGACTTTCCTGCACATCACTCAGTCAGTATGAGTACTCGTCGCTGTGCCAAAAGCTTGGCTTGCGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCCACATTAACACTCGCGGTTGCAGACCCAACTAACTT
ACAAGCGGAAGATGACTTTCGTTTTGCGACAGGGTTGCAAGTAGAGCTTGTTCTCGCTGATTTTCGCGAGCTGACCGCTG
CGATTCGGCGCTTATATGGCCGAGCACTTGGGCAAGAAAAGTCTGGATTAAAAGAGATCAACCAAGAAGAATTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGACGAATCTCCTGTTAGTCGTTATATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCCGAAAGGCGCTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCTACTCTATTTGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCAAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGGCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGATTTAGATACC
GCCGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGTGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

92.87

100

0.929
  pilB Vibrio parahaemolyticus RIMD 2210633

92.692

100

0.927
  pilB Vibrio cholerae strain A1552

74.377

100

0.745
  pilB Acinetobacter baumannii D1279779

51.731

92.692

0.48
  pilB Acinetobacter baylyi ADP1

47.67

99.465

0.474
  pilB Legionella pneumophila strain ERS1305867

48.803

96.791

0.472
  pilF Neisseria gonorrhoeae MS11

43.972

100

0.442
  pilB/pilB1 Synechocystis sp. PCC 6803

36.634

100

0.396
  pilF Thermus thermophilus HB27

36.299

100

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.315

99.643

0.362