Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H7683_RS12805 Genome accession   NZ_CP060288
Coordinates   2725856..2727937 (+) Length   693 a.a.
NCBI ID   WP_041977147.1    Uniprot ID   -
Organism   Pseudomonas mendocina strain CPS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2720856..2732937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7683_RS12785 (H7683_12780) pilM 2723019..2724083 (+) 1065 WP_013713726.1 pilus assembly protein PilM Machinery gene
  H7683_RS12790 (H7683_12785) - 2724083..2724667 (+) 585 WP_017363399.1 PilN domain-containing protein -
  H7683_RS12795 (H7683_12790) pilO 2724654..2725277 (+) 624 WP_017363398.1 type 4a pilus biogenesis protein PilO -
  H7683_RS12800 (H7683_12795) pilP 2725295..2725801 (+) 507 WP_221302232.1 type 4a pilus biogenesis lipoprotein PilP -
  H7683_RS12805 (H7683_12800) pilQ 2725856..2727937 (+) 2082 WP_041977147.1 type IV pilus secretin PilQ Machinery gene
  H7683_RS12810 (H7683_12805) aroK 2727943..2728461 (+) 519 WP_026042192.1 shikimate kinase AroK -
  H7683_RS12815 (H7683_12810) aroB 2728513..2729616 (+) 1104 WP_209449087.1 3-dehydroquinate synthase -
  H7683_RS12820 (H7683_12815) - 2729627..2731216 (+) 1590 WP_209449088.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 74754.53 Da        Isoelectric Point: 4.7428

>NTDB_id=475378 H7683_RS12805 WP_041977147.1 2725856..2727937(+) (pilQ) [Pseudomonas mendocina strain CPS5]
MNISLSQVGYALSMLFLSPALLAANLQHLDVASLPGDRVELKLRFDEPVAAPRGYTIEQPARIALDLPGVTSQLGSKGRE
LGMGNARSVTIVEAKDRTRLIVNLTSLAPYSTRVEGNDLFILVGEGSGVRGGGASRSVSSIKPPVVTGRAIRNLDFQRGE
QGEGNVVIDLADSSISPDIQDQGGKIRLDFAGTQLPESLRVRLDVKDFATPVQFVNASATSDKASILIEPVGMYDYLVYQ
ADNKLTVSVKPLSAADQEQRKADQFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVRGNITLRLQNVPWDQALD
LVLKTKGLDQRKVGNVLLVAPADEIAARERQELESRRQIAELAPLRRELIQVNYAKAADMAKLFQSVTSVDGAAEDRGSI
TVDDRTNSIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYEKQIGVNWRGARVGDNNFVIGGQGGNRPGAPG
YGQNPGVQNPTTSLGNFVDLGASSPTSGFGIGFITDNTILDLQLSAMEASGNGEVVSQPKVVTSDKETAKILKGSEVPYQ
EASSSGATSTSFKEAALSLEVTPQITPDNRILIEVKVTKDAPDFSQAAQTGGVPSIAKNEVNAKVLVSDGETIVIGGVFS
NTQSKSVDKVPFLGDLPYLGRLFRRDLVQDNKEELLIFLTPRIMNNQAIAVNQ

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=475378 H7683_RS12805 WP_041977147.1 2725856..2727937(+) (pilQ) [Pseudomonas mendocina strain CPS5]
ATGAATATCTCGCTCTCGCAAGTTGGTTATGCGTTGTCAATGCTGTTTCTGTCTCCAGCATTGCTGGCGGCCAATTTGCA
GCATCTTGACGTGGCTTCTTTGCCCGGTGATCGCGTTGAGCTTAAGCTACGGTTTGATGAACCTGTTGCTGCGCCTAGGG
GATATACAATCGAGCAGCCAGCTCGGATCGCACTTGATCTTCCAGGCGTAACCAGTCAATTGGGTAGTAAGGGCCGCGAG
CTTGGGATGGGTAACGCTAGAAGCGTAACTATTGTGGAGGCAAAGGATCGTACCCGCCTGATAGTTAATCTTACCTCGCT
GGCTCCTTATAGCACCCGTGTTGAGGGTAATGATCTCTTCATTTTGGTGGGTGAAGGATCGGGAGTGCGTGGGGGGGGGG
CGTCGCGCTCGGTCTCGTCGATCAAACCGCCGGTCGTGACTGGCAGGGCTATTCGTAATCTTGATTTTCAGAGAGGGGAG
CAGGGCGAGGGTAATGTTGTCATTGATCTTGCTGATTCCTCTATTAGTCCTGATATTCAAGATCAGGGAGGAAAGATTCG
TTTGGATTTTGCAGGGACTCAGTTGCCTGAATCGCTAAGAGTTCGTTTGGACGTAAAAGATTTCGCTACGCCAGTGCAGT
TTGTAAATGCTAGTGCTACTTCTGATAAGGCTAGTATCCTGATTGAGCCTGTCGGTATGTATGATTATCTGGTGTATCAA
GCCGACAATAAGCTTACTGTCAGTGTTAAACCGTTGAGTGCTGCGGATCAGGAACAGAGAAAAGCTGATCAGTTTGCATA
TTCGGGTGAGAAATTGTCTTTGAATTTTCAAGACATTGACGTCCGGTCTGTTCTGCAGTTAATTGCGGACTTTACTGATC
TGAATCTGGTTGCTAGTGATACTGTCAGGGGCAATATTACTCTGCGATTGCAGAATGTTCCTTGGGATCAGGCTCTTGAC
TTGGTTCTAAAAACCAAGGGGCTTGATCAGCGCAAGGTTGGCAATGTTCTTCTTGTTGCGCCGGCCGACGAAATTGCTGC
ACGCGAGCGGCAAGAGCTAGAGTCTCGTCGGCAGATTGCAGAGCTTGCTCCTCTGAGGCGGGAGCTGATCCAAGTCAACT
ACGCCAAAGCTGCGGATATGGCGAAGCTCTTTCAATCTGTAACCAGTGTTGATGGTGCTGCAGAGGATCGTGGGTCTATC
ACAGTTGATGATCGCACGAACAGCATAATTGCTTATCAGACTCAAGATCGTCTAGATGAGCTGCGACGTATTGTTGCTCA
GCTGGATATACCTGTTCGACAGGTCATGATAGAAGCGCGCATCGTCGAGGCTAATGTTGATTATGAGAAGCAGATTGGTG
TGAACTGGCGTGGGGCACGGGTTGGGGATAATAATTTTGTAATCGGTGGTCAGGGTGGTAATAGACCAGGTGCGCCAGGG
TATGGTCAAAATCCGGGGGTGCAGAACCCGACTACATCACTGGGTAATTTCGTCGACCTTGGTGCCTCCAGTCCTACTTC
TGGTTTTGGTATAGGATTCATTACTGACAACACCATTCTTGATCTTCAGCTTTCTGCAATGGAAGCAAGTGGTAATGGGG
AGGTTGTTTCTCAGCCCAAGGTTGTAACCTCTGATAAGGAGACTGCCAAGATCCTCAAGGGTTCTGAGGTTCCCTATCAA
GAAGCTAGTTCAAGTGGTGCTACTAGTACTTCGTTCAAAGAGGCTGCGCTCTCCCTTGAAGTTACTCCTCAGATTACACC
TGATAATCGTATTCTTATTGAGGTAAAAGTGACCAAGGATGCGCCTGACTTTTCGCAGGCTGCTCAGACCGGGGGCGTGC
CTTCAATTGCAAAAAATGAAGTCAATGCCAAGGTTCTTGTGTCTGATGGTGAAACAATCGTCATTGGTGGTGTCTTCTCT
AATACGCAGAGCAAGTCGGTTGATAAGGTTCCGTTCTTGGGGGATTTGCCATATCTGGGGCGTTTGTTCCGACGTGATCT
CGTGCAGGATAACAAGGAAGAGCTGTTGATTTTCCTTACTCCGCGTATCATGAACAACCAAGCCATAGCCGTGAATCAAT
GA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(526-684)

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(295-341)

Bacterial type II/III secretion system short domain (Secretin_N)

(369-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.652

100

0.773
  comQ Acinetobacter baylyi ADP1

41.355

100

0.431
  pilQ Acinetobacter baumannii D1279779

40.166

100

0.418
  pilQ Acinetobacter baumannii strain A118

40.166

100

0.418
  pilQ Legionella pneumophila strain ERS1305867

38.739

96.104

0.372
  pilQ Legionella pneumophila strain Lp02

38.739

96.104

0.372