Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H7683_RS12785 Genome accession   NZ_CP060288
Coordinates   2723019..2724083 (+) Length   354 a.a.
NCBI ID   WP_013713726.1    Uniprot ID   -
Organism   Pseudomonas mendocina strain CPS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718019..2729083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7683_RS12775 (H7683_12770) - 2718996..2720264 (+) 1269 WP_013713724.1 malic enzyme-like NAD(P)-binding protein -
  H7683_RS12780 (H7683_12775) - 2720426..2722837 (-) 2412 WP_209449086.1 penicillin-binding protein 1A -
  H7683_RS12785 (H7683_12780) pilM 2723019..2724083 (+) 1065 WP_013713726.1 pilus assembly protein PilM Machinery gene
  H7683_RS12790 (H7683_12785) - 2724083..2724667 (+) 585 WP_017363399.1 PilN domain-containing protein -
  H7683_RS12795 (H7683_12790) pilO 2724654..2725277 (+) 624 WP_017363398.1 type 4a pilus biogenesis protein PilO -
  H7683_RS12800 (H7683_12795) pilP 2725295..2725801 (+) 507 WP_221302232.1 type 4a pilus biogenesis lipoprotein PilP -
  H7683_RS12805 (H7683_12800) pilQ 2725856..2727937 (+) 2082 WP_041977147.1 type IV pilus secretin PilQ Machinery gene
  H7683_RS12810 (H7683_12805) aroK 2727943..2728461 (+) 519 WP_026042192.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37843.22 Da        Isoelectric Point: 4.4647

>NTDB_id=475377 H7683_RS12785 WP_013713726.1 2723019..2724083(+) (pilM) [Pseudomonas mendocina strain CPS5]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=475377 H7683_RS12785 WP_013713726.1 2723019..2724083(+) (pilM) [Pseudomonas mendocina strain CPS5]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCATACGCAGTCGAGCCGCTTCCGCCAAACGCAGTGGTCGAGAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCGCTTTCTCGCGTACTGGCCAAGGCCAAGAGTGGTGTCAAGACAGCAGCCGTT
GCCGTTGCTGGTTCAGCCGTCATCACCAAGACCATCGAGATGGAAGCCGGGCTTTCCGAGGATGATCTGGAGAACCAGCT
GAAGATTGAGGCCGACCAGTACATTCCCTACCCGCTGGAAGAGGTCGCCATCGACTTCGAAGTGCAGGGGCCCGCGCCGC
GTAACCCCGAGCGAGTCGAAGTGCTGCTGGCTGCATGCCGCAAGGAAAACGTCGAGGTCCGCGAAGCGGCGCTGGCGCTT
GCCGGCTTGACCGCAAAAGTGGTGGACGTCGAGGCGTACGCGCTGGAGCGTGCCTACAGCCTGCTGGAGGCGCAGCTGGG
TGGTGGGCACGACGAACTGACGGTTGCGGTGGTTGATATCGGTGCGACCATGACCACGCTTAGCGTTCTGCACAACGGCC
GCACCATCTATACCCGTGAGCAGCTATTCGGCGGCAAGCAACTGACGGAAGAAATCCAGCGCCGTTATGGCCTGTCCGTC
GAGGAAGCTGGTCTTGCCAAGAAGCAAGGTGGTCTTCCAGATGACTACGACAGTGAAGTATTGCAACCGTTCAAAGAGGC
GGTCGTTCAGCAGGTCTCTCGCTCGCTGCAGTTCTTCTTCGCTGCTGGGCAGTTCAACGATGTTGACTACATCCTCCTGG
CTGGCGGCACTGCGTCCATCCCTGATCTTGATCGGCTGATCCAGCAGAAGATCGGTACGCAGACGCTAGTGGCCAATCCC
TTTGCCGACATGGCGCTCAGCAGCAAGGTCAACGCCGGTGCGTTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449