Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H5647_RS09780 Genome accession   NZ_CP060092
Coordinates   2259236..2260960 (-) Length   574 a.a.
NCBI ID   WP_045858175.1    Uniprot ID   -
Organism   Teredinibacter purpureus strain Bs12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2254236..2265960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5647_RS09750 argJ 2254686..2255906 (+) 1221 WP_045858165.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  H5647_RS09755 mutT 2255907..2256314 (+) 408 WP_045858166.1 8-oxo-dGTP diphosphatase MutT -
  H5647_RS09760 yacG 2256329..2256523 (-) 195 WP_045858168.1 DNA gyrase inhibitor YacG -
  H5647_RS09765 coaE 2256520..2257110 (-) 591 WP_045858169.1 dephospho-CoA kinase -
  H5647_RS09770 pilD 2257114..2257962 (-) 849 WP_236075052.1 A24 family peptidase Machinery gene
  H5647_RS09775 pilC 2258004..2259230 (-) 1227 WP_045858173.1 type II secretion system F family protein Machinery gene
  H5647_RS09780 pilB 2259236..2260960 (-) 1725 WP_045858175.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H5647_RS09785 - 2261376..2261816 (+) 441 WP_045858177.1 pilin -
  H5647_RS09790 - 2261913..2263820 (+) 1908 WP_162926350.1 M48 family metallopeptidase -
  H5647_RS09795 - 2263834..2264700 (+) 867 WP_045858181.1 glycosyltransferase family 2 protein -
  H5647_RS09800 - 2264780..2265751 (+) 972 WP_045858183.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62555.39 Da        Isoelectric Point: 4.6952

>NTDB_id=473927 H5647_RS09780 WP_045858175.1 2259236..2260960(-) (pilB) [Teredinibacter purpureus strain Bs12]
MNTAISISGLARRLVRDNLISEDDAVKAIEGATRDKTPLPLYLVNNSSISGKQIAEAAADEFGTPLLDISALSKDAIPSG
VIDPKLIQKHHALPLFKRGNRLFIAVADPTNLHALDEIKFNTGLTTEALLAEADKLHASIEAFLTAEEESIGDALGDLDE
DGLDDLDIEAVDEDGPKGEDTSEADEAPIVRFVNKVLLDAIKIGASDIHFEPYEKTYRVRFRTDGILKEMARPPVNLAIR
LAARLKVMSQMDISERRVPQDGRIKMKISKNRAIDFRVNTLPTLFGEKVVLRILDPSSAKLGIDALGYEAEQKKMYLDAL
EQPQGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVNMNNRVGLTFAEALRSFLRQDPDIVMVG
EIRDIETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPTFNVATTVSLIIAQRLARRLCPACKIPADDIPDEILT
EEGFDKIGVERSEFTLFHPVGCEKCTDGYKGRVGIYEVVRITAEISRIIMEGGNSIQIADAARNAGFNDLRISALQKAAS
GLTSLEEANRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=473927 H5647_RS09780 WP_045858175.1 2259236..2260960(-) (pilB) [Teredinibacter purpureus strain Bs12]
ATGAATACAGCTATCAGTATTAGTGGTCTTGCGCGACGATTAGTGCGAGACAACCTTATATCGGAAGACGATGCCGTAAA
GGCCATTGAGGGCGCAACTCGAGACAAAACTCCACTGCCTCTCTACCTGGTCAACAATAGCTCTATTAGCGGCAAGCAAA
TCGCAGAAGCTGCCGCAGATGAGTTTGGCACCCCGTTGCTCGATATATCCGCATTAAGCAAAGATGCCATTCCTTCTGGG
GTTATCGACCCCAAACTCATCCAAAAACACCATGCCCTCCCTTTGTTTAAACGCGGTAACCGATTATTTATTGCCGTAGC
AGACCCCACTAACCTTCATGCACTCGACGAAATAAAATTTAACACGGGACTAACCACAGAAGCACTTTTGGCCGAAGCCG
ATAAGCTGCACGCATCTATCGAAGCCTTTCTAACGGCAGAAGAAGAAAGTATTGGAGATGCTCTTGGCGATTTAGACGAA
GACGGGCTGGATGACCTTGATATTGAAGCCGTTGACGAAGACGGCCCCAAAGGAGAGGATACCTCTGAAGCGGACGAAGC
TCCTATCGTACGATTTGTAAATAAGGTTTTGCTCGACGCCATCAAGATTGGCGCATCTGACATCCATTTTGAGCCCTATG
AAAAAACCTATCGAGTACGATTTAGAACGGATGGAATACTGAAAGAAATGGCACGGCCTCCAGTCAATCTTGCCATTCGC
TTAGCCGCTCGCCTAAAAGTTATGTCTCAGATGGATATATCAGAAAGACGTGTACCGCAAGACGGTCGAATCAAAATGAA
AATATCCAAAAATCGCGCAATCGATTTTCGGGTAAACACTCTACCCACGCTCTTCGGAGAAAAAGTAGTGCTTCGAATTT
TGGACCCCTCAAGCGCTAAGCTCGGCATCGACGCGCTCGGCTACGAAGCAGAGCAAAAGAAAATGTACTTAGATGCTTTA
GAGCAACCACAAGGTATGATTCTTGTAACCGGCCCAACAGGTTCAGGCAAAACAGTTTCGCTCTATACAGGCCTTAACAT
ACTTAATACCGCTGAACGCAATATCTCAACAGCGGAAGATCCAGTTGAGATCAACCTTGAGGGTATTAATCAGGTCAATA
TGAACAACCGCGTAGGCCTAACGTTCGCAGAGGCACTGCGCTCCTTCCTCCGTCAAGATCCTGATATTGTAATGGTTGGG
GAAATTCGAGATATAGAAACCGCCGAAATAGCCATAAAGGCGGCTCAAACAGGCCACCTTGTACTCTCTACACTTCATAC
AAACAGTGCACCAGAAACCTTAACCCGACTCCTGAATATGGGCGTTCCGACATTTAACGTTGCGACCACTGTAAGCCTTA
TTATCGCTCAACGTTTGGCAAGAAGGCTTTGTCCCGCTTGCAAAATACCCGCCGACGATATTCCAGATGAAATTCTAACA
GAAGAAGGCTTCGACAAGATTGGTGTAGAACGCTCTGAATTTACGTTATTTCATCCGGTAGGGTGTGAAAAATGTACTGA
CGGTTATAAAGGCCGTGTGGGCATCTATGAAGTCGTTCGAATTACAGCTGAAATTTCACGCATCATTATGGAAGGCGGAA
ATTCAATTCAGATAGCCGATGCTGCGCGTAATGCAGGGTTCAACGATCTAAGAATATCAGCGCTTCAAAAAGCAGCCTCA
GGCCTTACTAGCCTTGAAGAAGCCAATCGAGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.862

100

0.603
  pilB Acinetobacter baylyi ADP1

59.201

100

0.594
  pilB Legionella pneumophila strain ERS1305867

56.491

99.303

0.561
  pilB Vibrio cholerae strain A1552

50.174

100

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.912

98.78

0.493
  pilB Vibrio campbellii strain DS40M4

51.215

93.206

0.477
  pilF Neisseria gonorrhoeae MS11

47.535

98.955

0.47
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.366

93.38

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

41.317

87.282

0.361