Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H5715_RS01805 Genome accession   NZ_CP060084
Coordinates   431249..432976 (-) Length   575 a.a.
NCBI ID   WP_075185850.1    Uniprot ID   -
Organism   Teredinibacter haidensis strain Bs08     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426249..437976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5715_RS01775 argJ 426715..427935 (+) 1221 WP_075185856.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  H5715_RS01780 mutT 427935..428339 (+) 405 WP_075185855.1 8-oxo-dGTP diphosphatase MutT -
  H5715_RS01785 yacG 428336..428536 (-) 201 WP_075185854.1 DNA gyrase inhibitor YacG -
  H5715_RS01790 coaE 428533..429123 (-) 591 WP_075185853.1 dephospho-CoA kinase -
  H5715_RS01795 pilD 429130..430017 (-) 888 WP_075185852.1 A24 family peptidase Machinery gene
  H5715_RS01800 pilC 430017..431243 (-) 1227 WP_075185851.1 type II secretion system F family protein Machinery gene
  H5715_RS01805 pilB 431249..432976 (-) 1728 WP_075185850.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H5715_RS01810 pilE 433390..433827 (+) 438 WP_075185849.1 pilin Machinery gene
  H5715_RS01815 - 434063..435952 (+) 1890 WP_185906581.1 hypothetical protein -
  H5715_RS01820 - 436041..436898 (+) 858 WP_075185847.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62707.45 Da        Isoelectric Point: 4.8837

>NTDB_id=473744 H5715_RS01805 WP_075185850.1 431249..432976(-) (pilB) [Teredinibacter haidensis strain Bs08]
MNTATSLTGLARRLVRDNLISEDEAQKATEAAAKAKTPLPLYLVNNSAIDAKSMAEAAADEFGTPLYDLASLSTESIPSG
IVDSKLIHKHHALPLFKRGNRLFVALADPTNLHALDEIKFNTGLNTEVLLAEADKLHTAIEAFLKAEEESIGDALGGLDE
EALDDLDIEAVDESNQQGTDDSSEADEAPIVRFVNKVLLDAIKIGASDVHFEPYEKSYRVRFRTDGILKEMARPPTNLAT
RLAARLKVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNSLPTLFGEKIVLRILDPSSAKLGIDALGYEEDQKKMYMDA
LAQPQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGVNQVNMNTRVGLTFAEALRSFLRQDPDIVMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPTFNVATTVSIIIAQRLARRLCSACKKPADDIPEEIL
SEEGFDTIGVPREEFTLFHPVGCNKCSDGYKGRVGIYEVVRITPEISRIIMEGGNSIQIADAARKAGFNDLRISALRKAA
AGLTSLEEANRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=473744 H5715_RS01805 WP_075185850.1 431249..432976(-) (pilB) [Teredinibacter haidensis strain Bs08]
ATGAATACAGCAACCAGTCTTACCGGCCTTGCACGTCGCTTAGTTCGAGATAACCTCATCTCGGAAGACGAAGCGCAAAA
AGCAACCGAAGCCGCGGCCAAAGCGAAGACTCCACTTCCCTTGTACCTCGTCAACAACAGTGCTATCGACGCCAAATCCA
TGGCTGAAGCTGCAGCCGATGAGTTTGGAACCCCCTTGTACGACCTCGCATCCCTGAGCACCGAGTCAATCCCCTCGGGT
ATAGTCGACTCTAAACTGATTCACAAGCACCACGCGCTTCCACTGTTCAAGCGAGGCAACAGACTATTCGTTGCATTAGC
AGACCCAACCAACCTGCACGCGCTCGACGAAATTAAATTCAACACCGGCTTAAATACCGAAGTCTTATTAGCCGAAGCCG
ATAAACTCCATACGGCAATAGAAGCTTTTCTGAAAGCTGAAGAAGAAAGTATCGGCGATGCGCTTGGAGGGCTAGACGAA
GAGGCGCTCGACGACCTTGATATCGAAGCCGTGGATGAAAGCAACCAACAGGGTACAGACGATAGTTCCGAAGCCGATGA
GGCGCCCATTGTTCGCTTTGTCAATAAAGTCCTTTTGGACGCCATAAAGATCGGCGCATCCGACGTTCATTTTGAGCCCT
ACGAGAAATCTTACCGGGTACGATTTCGTACCGATGGCATCCTGAAAGAAATGGCTCGCCCGCCCACAAACCTTGCGACT
CGATTGGCGGCCAGGTTAAAAGTGATGTCACAAATGGACATCTCTGAACGACGCGTTCCACAAGACGGTCGTATTAAGAT
GAAAATTTCCAAAACCCGGGCCATCGACTTTCGTGTGAACAGTCTTCCAACACTTTTTGGTGAAAAGATTGTTCTTCGAA
TTCTTGACCCGTCCAGTGCAAAACTGGGTATTGACGCCCTCGGTTACGAAGAAGACCAGAAAAAAATGTATATGGATGCA
TTGGCCCAGCCCCAGGGAATGATCTTAGTTACCGGCCCTACCGGTTCGGGTAAGACAGTTTCTCTCTATACCGGATTAAA
CATTCTAAACACAACCGAACGCAATATTTCGACTGCAGAAGACCCTGTGGAAATTAACCTGGAGGGGGTAAACCAGGTCA
ACATGAATACGAGGGTTGGACTTACCTTCGCGGAGGCATTACGATCATTTCTGCGCCAAGACCCCGACATTGTGATGGTA
GGCGAGATCCGAGACCTCGAAACGGCCGAAATTGCGATTAAAGCCGCTCAAACAGGCCACTTGGTTTTATCTACTTTACA
CACTAACAGCGCCCCCGAAACCCTCACCCGCCTGCTCAATATGGGTGTACCGACATTTAATGTTGCAACAACCGTCAGTA
TTATTATTGCGCAACGCCTAGCCAGAAGACTGTGCAGCGCCTGCAAAAAACCGGCGGATGATATTCCCGAAGAAATTCTC
TCTGAAGAAGGTTTCGACACTATTGGGGTCCCTCGGGAAGAGTTCACACTGTTCCACCCCGTGGGTTGCAACAAGTGCAG
TGACGGCTATAAGGGCCGTGTAGGTATTTATGAAGTTGTGCGCATTACACCCGAGATTTCACGCATTATTATGGAAGGTG
GCAATTCTATTCAAATTGCCGATGCCGCCCGTAAAGCGGGTTTTAACGATTTACGAATATCGGCGCTTCGCAAGGCGGCA
GCAGGGCTAACCAGCCTCGAAGAAGCCAACCGAGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.391

100

0.614
  pilB Acinetobacter baylyi ADP1

60

100

0.6
  pilB Legionella pneumophila strain ERS1305867

53.927

99.652

0.537
  pilB Vibrio cholerae strain A1552

51.056

98.783

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

49.558

98.261

0.487
  pilB Vibrio campbellii strain DS40M4

48.854

98.609

0.482
  pilF Neisseria gonorrhoeae MS11

46.842

99.13

0.464
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.679

97.391

0.367