Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H6S61_RS07355 Genome accession   NZ_CP060047
Coordinates   1455800..1457572 (-) Length   590 a.a.
NCBI ID   WP_185892038.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1450800..1462572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS07335 (H6S61_07335) - 1451502..1452329 (-) 828 WP_185892035.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  H6S61_RS07340 (H6S61_07340) - 1452409..1453905 (-) 1497 WP_185892036.1 AAA family ATPase -
  H6S61_RS07345 (H6S61_07345) aroB 1453956..1455047 (-) 1092 WP_185892037.1 3-dehydroquinate synthase -
  H6S61_RS07350 (H6S61_07350) aroK 1455080..1455598 (-) 519 WP_011079357.1 shikimate kinase AroK -
  H6S61_RS07355 (H6S61_07355) pilQ 1455800..1457572 (-) 1773 WP_185892038.1 type IV pilus secretin PilQ family protein Machinery gene
  H6S61_RS07360 (H6S61_07360) pilP 1457586..1458098 (-) 513 WP_017419659.1 pilus assembly protein PilP Machinery gene
  H6S61_RS07365 (H6S61_07365) pilO 1458088..1458678 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  H6S61_RS07370 (H6S61_07370) pilN 1458671..1459261 (-) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  H6S61_RS07375 (H6S61_07375) pilM 1459251..1460258 (-) 1008 WP_185892039.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64303.40 Da        Isoelectric Point: 4.5077

>NTDB_id=473545 H6S61_RS07355 WP_185892038.1 1455800..1457572(-) (pilQ) [Vibrio vulnificus strain 2497-87]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGDSGSGGSGDEGGSNSIDDFLNVN
LAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSFKKAV
LSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPLLGDL
PLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=473545 H6S61_RS07355 WP_185892038.1 1455800..1457572(-) (pilQ) [Vibrio vulnificus strain 2497-87]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAATGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACCGTGGTGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTTAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTTTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTCAGCGGTGAATTTCGTCACGACTATCGTCTTT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAGCAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGACATCA
TTCTTCAAGTGAAAGGTCTAGACAAACGTGTTGAAGGCAATGTTGTGCTCATTGCTCCTCAAGATGAACTGGATTTACGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATTAAAGTCAACTTTGC
CAAAGCGTCAGACATTGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAACGTGGTAGTGTGGCGATTG
ATGAGCGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCACGCATTGTCACGGTGAATGAAGGCAATCTTGATGAGCTTGGTGTCCGTTG
GGGGTTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCTATCGAAAACAACCTAGCAACCATTGGTCTCTACAATG
CAGGCAGTGGTGACTCAGGTAGCGGTGGCAGTGGTGATGAAGGCGGGTCGAACTCCATTGATGATTTCCTCAATGTCAAT
CTTGCTGCGACTTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTTGTTGGATCTCGA
GCTATCAGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATAAGAAGCCTGCTT
ATATCGAGCAGGGTACAGAGATTCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCCTTTAAGAAAGCTGTG
CTTAGCCTGAAAGTAACCCCTCAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACAAGATAGACCTGG
CGAAATTGTCAAAACAGGTACTGGGGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCTTGGTCAATAATG
GTGAAACGGTAGTGTTAGGCGGTATTTTCCAACACAGCATAACCAATACCACGGACAAGGTTCCTCTGTTAGGCGATTTA
CCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTAGGCAAACGTGAATTGCTGATATTTGTCACACCTAAGGT
TATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(430-588)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

78.328

99.322

0.778
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.573

99.322

0.741
  pilQ Vibrio cholerae strain A1552

74.573

99.322

0.741
  pilQ Legionella pneumophila strain Lp02

41.727

94.237

0.393
  pilQ Legionella pneumophila strain ERS1305867

41.727

94.237

0.393
  pilQ Pseudomonas aeruginosa PAK

39.716

95.593

0.38
  comQ Acinetobacter baylyi ADP1

37.245

99.661

0.371