Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H6S61_RS07375 Genome accession   NZ_CP060047
Coordinates   1459251..1460258 (-) Length   335 a.a.
NCBI ID   WP_185892039.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1454251..1465258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS07350 (H6S61_07350) aroK 1455080..1455598 (-) 519 WP_011079357.1 shikimate kinase AroK -
  H6S61_RS07355 (H6S61_07355) pilQ 1455800..1457572 (-) 1773 WP_185892038.1 type IV pilus secretin PilQ family protein Machinery gene
  H6S61_RS07360 (H6S61_07360) pilP 1457586..1458098 (-) 513 WP_017419659.1 pilus assembly protein PilP Machinery gene
  H6S61_RS07365 (H6S61_07365) pilO 1458088..1458678 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  H6S61_RS07370 (H6S61_07370) pilN 1458671..1459261 (-) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  H6S61_RS07375 (H6S61_07375) pilM 1459251..1460258 (-) 1008 WP_185892039.1 type IV pilus assembly protein PilM Machinery gene
  H6S61_RS07380 (H6S61_07380) - 1460398..1462911 (+) 2514 WP_017419655.1 PBP1A family penicillin-binding protein -
  H6S61_RS07385 (H6S61_07385) oxyR 1462980..1463879 (-) 900 WP_185892040.1 DNA-binding transcriptional regulator OxyR -
  H6S61_RS07390 (H6S61_07390) - 1464153..1464425 (+) 273 WP_017419653.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37601.00 Da        Isoelectric Point: 6.2686

>NTDB_id=473549 H6S61_RS07375 WP_185892039.1 1459251..1460258(-) (pilM) [Vibrio vulnificus strain 2497-87]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSCRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=473549 H6S61_RS07375 WP_185892039.1 1459251..1460258(-) (pilM) [Vibrio vulnificus strain 2497-87]
ATGGGTAAACCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAGTTGCCTCTTTTCCGTTATCAGGTGTCACTGGCGGTACCCGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAACGAGAGTTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGAGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTACATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGACAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGTGTCGCAACGAACCACCTTTGGTTAAAGAGTTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAGCTGACAGAGCGAGTTCGTCGGCAATTGCAGTTGTTCCAATCCATGGATAGCCAAAGCAAAGTGAATGGCCTTTGGAT
CTGCGGTGAAGGCGCGATTACGCCGTTGTTGAGTGAAACTTTGCAGAGAAAGCTGAGTTTGGAGTGTGAAGTCCTTAATC
CTCTGGCTCTATTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTCGCCGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499