Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HNQ25_RS21790 Genome accession   NZ_CP060009
Coordinates   4657622..4659733 (-) Length   703 a.a.
NCBI ID   WP_179545997.1    Uniprot ID   -
Organism   Pseudomonas sediminis strain B10D7D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4652622..4664733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNQ25_RS21775 (HNQ25_21775) - 4654388..4655932 (-) 1545 WP_179545995.1 AAA family ATPase -
  HNQ25_RS21780 (HNQ25_21780) aroB 4655943..4657046 (-) 1104 WP_179545996.1 3-dehydroquinate synthase -
  HNQ25_RS21785 (HNQ25_21785) aroK 4657098..4657616 (-) 519 WP_099522785.1 shikimate kinase AroK -
  HNQ25_RS21790 (HNQ25_21790) pilQ 4657622..4659733 (-) 2112 WP_179545997.1 type IV pilus secretin PilQ Machinery gene
  HNQ25_RS21795 (HNQ25_21795) pilP 4659789..4660313 (-) 525 WP_099522787.1 type 4a pilus biogenesis lipoprotein PilP -
  HNQ25_RS21800 (HNQ25_21800) pilO 4660310..4660933 (-) 624 WP_179545998.1 type 4a pilus biogenesis protein PilO -
  HNQ25_RS21805 (HNQ25_21805) pilN 4660930..4661502 (-) 573 WP_179545999.1 type 4a pilus biogenesis protein PilN -
  HNQ25_RS21810 (HNQ25_21810) pilM 4661502..4662566 (-) 1065 WP_017678539.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 76028.21 Da        Isoelectric Point: 4.7408

>NTDB_id=473283 HNQ25_RS21790 WP_179545997.1 4657622..4659733(-) (pilQ) [Pseudomonas sediminis strain B10D7D]
MNGSLSRLSVALLAALLSPAVLAANLQDLNVASLPGDRVELKLSFDEPVTAPRGYTIEQPARIALDLPGVSNKLGSRNRE
LGVGNARSVTIVEAKDRTRLIVNLTNLAPYSTRVEGNDLYVLVGDASAAAARPSASTPASVTSVPPKKTYGPQAKAISNI
DFQRGEQGEGNIVITLSDASISPDIQEQGGKIRLDFAKTELPESLRVRLDVKDFATPVQFVSATGSADKTSILVEPVGLY
DFLAYQTENKLTLSVKPLSQDDVERRKAERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVP
WDQALDLVLKTKGLDKRQIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADMAKLFQSVTSADGSA
DVRGSVTVDDRTNSIIAYQTQERLDELRRIIAQLDIPVRQVMIEARIVEANVNYDKALGVRWGGASRRGNWSLYGKDGAT
SFDEGQAYLPGSDTVGDFTLEDGVAPVPFVDMGVAASTSGIGIGFLTDNLVLDLQLSAMESSGNGEIVSQPKVVTADKET
AKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFQRAINGVPPINKNEVNAKVLVSDG
ETIVIGGVFENTQTKAVEKVPFLGDVPYLGRLFRRDIVRDNKTELLVFLTPRIMNNQAIAVNR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=473283 HNQ25_RS21790 WP_179545997.1 4657622..4659733(-) (pilQ) [Pseudomonas sediminis strain B10D7D]
ATGAACGGCTCTCTTTCGCGTCTTAGCGTCGCCCTGTTGGCGGCGCTGCTGTCGCCCGCGGTATTGGCGGCCAATCTCCA
GGATTTGAATGTCGCCAGCTTGCCCGGTGATCGGGTTGAGCTGAAGCTGTCCTTCGATGAGCCGGTAACGGCTCCTCGCG
GCTATACCATCGAGCAGCCTGCGCGTATTGCTCTGGACCTGCCGGGGGTATCGAACAAGCTCGGCTCCAGGAATCGTGAG
CTGGGTGTCGGCAATGCGCGTAGCGTCACCATCGTCGAAGCCAAGGACCGTACCCGTCTGATCGTCAACCTCACCAACCT
GGCGCCATACAGCACCCGAGTTGAGGGTAACGATCTGTATGTCCTGGTTGGCGATGCCTCTGCAGCCGCAGCGCGACCCT
CTGCTTCGACGCCTGCGTCGGTAACGTCTGTTCCGCCCAAGAAAACCTACGGGCCGCAGGCGAAAGCAATCAGCAATATC
GACTTCCAGCGTGGCGAGCAGGGCGAGGGCAATATCGTCATTACTCTTTCCGATGCCTCGATAAGCCCTGACATTCAGGA
GCAGGGTGGGAAAATTCGTCTGGACTTTGCCAAGACTGAGCTGCCTGAGTCCCTGCGTGTACGCCTAGATGTGAAAGATT
TCGCAACGCCCGTGCAGTTCGTCAGCGCCACGGGTAGTGCAGACAAAACCAGTATTCTGGTCGAGCCGGTCGGTTTGTAT
GATTTCTTGGCCTATCAGACCGAGAACAAGCTGACCTTGAGCGTCAAGCCTCTCAGTCAGGACGATGTCGAGCGCCGTAA
GGCGGAGCGTTTCGCTTATACCGGGGAGAAGTTGTCGCTCAACTTCCAGGATATTGATGTACGTTCCGTTCTGCAGTTGA
TCGCCGATTTCACCGATCTGAATCTGGTGGCGAGTGACACTGTCGCCGGCAACATTACTCTGCGTCTGCAGAATGTGCCT
TGGGATCAGGCACTTGATCTGGTGTTGAAAACCAAGGGTCTGGATAAGCGTCAGATTGGTAACGTCCTGCTGGTGGCCCC
GGCGGACGAAATCGCTGCTCGTGAGCGCCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGAACTCGCTCCGCTGCGTCGCG
AGCTGATTCAGGTGAACTATGCCAAGGCGGCCGATATGGCCAAGCTGTTCCAGTCGGTAACCAGTGCTGATGGTTCGGCG
GACGTACGTGGTTCTGTCACCGTCGATGATCGAACCAACAGCATCATTGCCTATCAGACTCAGGAGCGCCTTGACGAACT
GCGTCGTATCATCGCTCAGTTAGACATTCCTGTGCGTCAGGTGATGATCGAGGCTCGCATCGTTGAAGCGAACGTGAATT
ACGACAAGGCTCTGGGGGTTCGCTGGGGGGGCGCGAGTCGTCGGGGAAATTGGAGTCTTTACGGTAAGGACGGTGCAACT
TCCTTTGACGAGGGGCAGGCCTACTTGCCTGGTTCTGACACCGTAGGGGATTTCACCTTGGAAGATGGTGTTGCTCCTGT
TCCTTTCGTCGATATGGGTGTTGCAGCTAGTACTTCGGGTATCGGTATTGGCTTCCTGACGGACAATCTCGTTCTGGATC
TGCAGCTTTCGGCCATGGAAAGCTCCGGTAATGGTGAAATTGTTTCTCAGCCCAAGGTAGTTACTGCAGATAAAGAAACT
GCAAAGATTCTCAAAGGGCAGGAGGTTCCGTATCAAGAGGCCAGCTCCAGCGGGGCTACTTCCACATCCTTCAAAGAGGC
TGCGCTGTCTCTTGAAGTGACGCCTCAGATCACTCCAGATAATCGAATTATTATGGAAGTGAAAGTTACCAAGGACGCAC
CTGACTTCCAGCGAGCTATCAACGGTGTTCCACCGATTAACAAAAATGAGGTCAACGCCAAGGTTTTGGTTTCTGATGGC
GAAACAATCGTCATCGGTGGCGTTTTCGAAAACACCCAAACCAAAGCCGTTGAGAAGGTTCCGTTCCTTGGGGATGTACC
CTACCTCGGACGTCTTTTCCGTCGTGACATCGTTCGTGACAACAAGACTGAGTTGCTGGTTTTCCTCACTCCGCGCATCA
TGAACAACCAAGCCATTGCCGTGAACCGATGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(538-694)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Secretin and TonB N terminus short domain (STN)

(301-347)

AMIN domain (AMIN)

(37-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.05

100

0.772
  comQ Acinetobacter baylyi ADP1

42.407

99.289

0.421
  pilQ Acinetobacter baumannii strain A118

39.919

100

0.42
  pilQ Acinetobacter baumannii D1279779

39.919

100

0.42
  pilQ Legionella pneumophila strain Lp02

38.462

96.159

0.37
  pilQ Legionella pneumophila strain ERS1305867

38.222

96.017

0.367