Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HNQ25_RS21810 Genome accession   NZ_CP060009
Coordinates   4661502..4662566 (-) Length   354 a.a.
NCBI ID   WP_017678539.1    Uniprot ID   A0A1G6YHQ2
Organism   Pseudomonas sediminis strain B10D7D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4656502..4667566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNQ25_RS21785 (HNQ25_21785) aroK 4657098..4657616 (-) 519 WP_099522785.1 shikimate kinase AroK -
  HNQ25_RS21790 (HNQ25_21790) pilQ 4657622..4659733 (-) 2112 WP_179545997.1 type IV pilus secretin PilQ Machinery gene
  HNQ25_RS21795 (HNQ25_21795) pilP 4659789..4660313 (-) 525 WP_099522787.1 type 4a pilus biogenesis lipoprotein PilP -
  HNQ25_RS21800 (HNQ25_21800) pilO 4660310..4660933 (-) 624 WP_179545998.1 type 4a pilus biogenesis protein PilO -
  HNQ25_RS21805 (HNQ25_21805) pilN 4660930..4661502 (-) 573 WP_179545999.1 type 4a pilus biogenesis protein PilN -
  HNQ25_RS21810 (HNQ25_21810) pilM 4661502..4662566 (-) 1065 WP_017678539.1 pilus assembly protein PilM Machinery gene
  HNQ25_RS21815 (HNQ25_21815) - 4662748..4665159 (+) 2412 WP_179546000.1 penicillin-binding protein 1A -
  HNQ25_RS21820 (HNQ25_21820) - 4665269..4666537 (-) 1269 WP_179546001.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37829.19 Da        Isoelectric Point: 4.4647

>NTDB_id=473284 HNQ25_RS21810 WP_017678539.1 4661502..4662566(-) (pilM) [Pseudomonas sediminis strain B10D7D]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYSVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=473284 HNQ25_RS21810 WP_017678539.1 4661502..4662566(-) (pilM) [Pseudomonas sediminis strain B10D7D]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCTTATTCCGTCGAGCCGCTTCCACCTAACGCAGTGGTAGAGAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCGCTCTCGCGCGTGCTGGCCAAGGCCAAGAGCGGCGTCAAGACGGCTGCGGTT
GCTGTGGCTGGTTCGGCGGTCATCACCAAAACCATCGAAATGGAAGCCGGCCTTTCCGAGGATGACCTGGAAAACCAGCT
GAAGATCGAGGCTGACCAATACATCCCCTACCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCCCGGCGCCGC
GTAACCCAGAGCGGGTTGAGGTGCTGCTGGCCGCATGCCGTAAGGAGAACGTGGAGGTCCGCGAAGCAGCCCTCGCGCTC
GCCGGGCTTACTGCGAAAGTGGTCGATGTCGAGGCCTATGCCCTCGAGCGCGCTTACGGTTTGCTGGAGGCGCAGCTGGG
TGGCGGTCACGATGAGTTGACCGTCGCGGTGGTGGATATCGGCGCGACGATGACCACGCTCAGTGTTCTGCACAACGGCC
GCACCATCTACACCCGCGAGCAGCTTTTCGGTGGCAAGCAACTGACCGAGGAAATCCAGCGCCGCTACGGCTTGTCGGTC
GAGGAAGCTGGTCTTGCCAAGAAACAAGGTGGTCTTCCAGATGACTACGACAGTGAGGTTTTGCAACCGTTCAAAGAAGC
TGTCGTTCAGCAGGTTTCGCGCTCGCTGCAGTTCTTCTTCGCCGCCGGGCAATTCAATGATGTCGACTACATCCTCCTGG
CTGGCGGTACCGCGTCCATTCCTGACCTCGATCGGCTGATCCAGCAGAAGATCGGTACGCAGACGTTAGTGGCCAATCCC
TTTGCCGATATGGCGCTGAGCAGCAAGGTCAATGCCGGTGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G6YHQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449