Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HNQ25_RS20330 Genome accession   NZ_CP060009
Coordinates   4323396..4325099 (+) Length   567 a.a.
NCBI ID   WP_179545795.1    Uniprot ID   -
Organism   Pseudomonas sediminis strain B10D7D     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4318396..4330099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNQ25_RS20315 (HNQ25_20315) nadC 4319672..4320520 (+) 849 WP_179545793.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HNQ25_RS20320 (HNQ25_20320) - 4321076..4322521 (-) 1446 WP_244968160.1 O-antigen ligase family protein -
  HNQ25_RS20325 (HNQ25_20325) - 4322730..4323173 (-) 444 WP_179545794.1 pilin -
  HNQ25_RS20330 (HNQ25_20330) pilB 4323396..4325099 (+) 1704 WP_179545795.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNQ25_RS20335 (HNQ25_20335) pilC 4325102..4326322 (+) 1221 WP_185806061.1 type II secretion system F family protein Machinery gene
  HNQ25_RS20340 (HNQ25_20340) pilD 4326323..4327192 (+) 870 WP_179545796.1 A24 family peptidase Machinery gene
  HNQ25_RS20345 (HNQ25_20345) coaE 4327370..4327978 (+) 609 WP_143506136.1 dephospho-CoA kinase -
  HNQ25_RS20350 (HNQ25_20350) yacG 4327975..4328169 (+) 195 WP_179545797.1 DNA gyrase inhibitor YacG -
  HNQ25_RS20355 (HNQ25_20355) - 4328317..4329447 (-) 1131 WP_179545798.1 IS481 family transposase -
  HNQ25_RS20360 (HNQ25_20360) - 4329558..4329773 (-) 216 WP_179545799.1 hypothetical protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62484.41 Da        Isoelectric Point: 5.4687

>NTDB_id=473280 HNQ25_RS20330 WP_179545795.1 4323396..4325099(+) (pilB) [Pseudomonas sediminis strain B10D7D]
MNDSISLSGLAKQMVLAELLDEKAAQQAQQQAQRNKLSLVTYLVQNKLVKSRALAELASEQFGVSFLDLNTLDKESQPRD
LISEKLVRQHRVLPLWRRGNKLFVGLSDPSNHQAVTDVQFSTGLNTEAILVEDDKLGDAIDKFFDSANSGMEDLADVDLD
SLDVQAVDDDKTVGDGGADADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMKISKNKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEVNVPRETLLAEGFP
EDKIGTFKIYGPAGCENCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIATQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=473280 HNQ25_RS20330 WP_179545795.1 4323396..4325099(+) (pilB) [Pseudomonas sediminis strain B10D7D]
ATGAACGACAGCATTTCCCTTTCCGGCCTGGCCAAGCAGATGGTCCTTGCCGAGTTGTTGGATGAAAAAGCCGCCCAACA
GGCTCAGCAACAGGCCCAGCGCAACAAACTGTCGCTGGTCACTTACCTGGTGCAAAACAAGCTGGTAAAGAGTCGCGCCC
TGGCCGAACTGGCCTCGGAGCAGTTCGGCGTTTCCTTTCTCGACCTCAATACCCTCGACAAGGAAAGCCAACCACGTGAC
CTGATCAGCGAAAAGCTGGTACGCCAACACCGCGTACTGCCTTTATGGCGTCGCGGCAACAAGCTGTTCGTCGGCCTTTC
CGACCCGTCCAATCACCAGGCCGTCACCGACGTACAGTTCAGCACCGGCCTGAATACAGAGGCCATCCTGGTCGAGGACG
ACAAGCTCGGCGATGCCATCGACAAGTTCTTCGACTCAGCCAATAGCGGCATGGAAGATCTGGCCGATGTCGACCTCGAC
AGCCTGGATGTACAGGCCGTGGATGACGACAAAACCGTAGGCGACGGCGGCGCTGACGCTGACGATGCGCCCGTGGTGCG
CTTCGTCAACAAGATGCTACTGGATGCGATTAAAGGCGGCTCCTCCGACCTGCACTTCGAGCCTTACGAAAAAGCCTACC
GGGTACGCTTTCGCACCGACGGCATCCTGCATGAGGTTGCCCGCCCGCCCATTCAACTTGCGCCGCGTATTTCCGCCCGC
CTCAAGGTGATGGCCGCGCTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAAATTTCCAAGAA
CAAGGCCATCGACTTTCGCGTCAACACCCTGCCCACGCTGTGGGGCGAAAAGATCGTGATGCGAATTCTTGACCCCACCA
GCGCACAGATGGGTATCGATGCACTGGGGTACGAGGAGGAACAGAAAGAACTGTATATGTCTGCCCTCAAGCAGCCGCAG
GGCATGATTCTGGTCACCGGCCCCACCGGTTCGGGGAAAACCGTATCGCTCTATACCGGCCTGAACATCCTCAATACGCC
TGATGTGAATATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAGGGTATCAACCAGGTCAACGTCAACCCCAAGC
AGGGCATGGATTTCACCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATATCATCATGGTCGGTGAGATCCGCGAC
CTGGACACGGCCTCCATAGCCGTCAAGGCCGCGCAAACCGGGCACATGGTGATGTCGACTCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCCTGCGCAATATGGGTGTGCCATCTTTCAACATCGCCACCTCGGTCAACCTGATCATTGCCCAGC
GCCTGGCGCGCAAGCTATGCAACAGCTGCAAGAAGGAAGTGAACGTACCGCGCGAAACCCTGCTGGCCGAAGGCTTCCCC
GAGGACAAGATCGGGACTTTCAAGATTTATGGCCCTGCTGGCTGTGAAAACTGTAAGGGCGGTTACAAAGGCCGCGTCGG
TATTTATGAAGTGGTTAAAAACACGCCCAACCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCACGC
AGATGCGCAAAGACGGCTTTAATGACTTACGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTCACCAGCCTTGAGGAA
GTCAACCGCGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.23

99.647

0.58
  pilB Acinetobacter baumannii D1279779

57.719

100

0.58
  pilB Legionella pneumophila strain ERS1305867

54.867

99.647

0.547
  pilB Vibrio cholerae strain A1552

48.936

99.471

0.487
  pilF Neisseria gonorrhoeae MS11

48.135

99.295

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

50

95.238

0.476
  pilB Vibrio campbellii strain DS40M4

49.168

95.414

0.469
  pilF Thermus thermophilus HB27

37.368

100

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.076

93.298

0.374