Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H5J27_RS30330 Genome accession   NZ_CP059995
Coordinates   6422627..6424771 (-) Length   714 a.a.
NCBI ID   WP_042170908.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY3045     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6417627..6429771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5J27_RS30315 (H5J27_30315) - 6419279..6420934 (-) 1656 WP_033967775.1 AAA family ATPase -
  H5J27_RS30320 (H5J27_30320) aroB 6420941..6422047 (-) 1107 WP_033977028.1 3-dehydroquinate synthase -
  H5J27_RS30325 (H5J27_30325) aroK 6422097..6422615 (-) 519 WP_003095833.1 shikimate kinase AroK -
  H5J27_RS30330 (H5J27_30330) pilQ 6422627..6424771 (-) 2145 WP_042170908.1 type 4a pilus secretin PilQ Machinery gene
  H5J27_RS30335 (H5J27_30335) pilP 6424825..6425349 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  H5J27_RS30340 (H5J27_30340) pilO 6425346..6425969 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  H5J27_RS30345 (H5J27_30345) pilN 6425966..6426562 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  H5J27_RS30350 (H5J27_30350) pilM 6426562..6427626 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77481.07 Da        Isoelectric Point: 5.3301

>NTDB_id=473135 H5J27_RS30330 WP_042170908.1 6422627..6424771(-) (pilQ) [Pseudomonas aeruginosa strain NY3045]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIREQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SEGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=473135 H5J27_RS30330 WP_042170908.1 6422627..6424771(-) (pilQ) [Pseudomonas aeruginosa strain NY3045]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGAGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGCTGATCATCAACCTGACCACGTT
GTCGTCCTATACCACCCGCGTGGAAGGCAACAATCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCCTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCGGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACTCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGATGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTGCTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGTCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGAGGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAGCTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CGCGCATCGTCGAGGCCAACGTCGGCTACGACAAGAGCCTCGGGGTGCGCTGGGGCGGCGCCTACCACAAGGGCAACTGG
AGCGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCCACCTCGGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTCAAGGTGACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGATGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTGCCGTTCCTCGGTGAACTGCCTTACCTGGGGCGGCTGTTCCGGCGCGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(549-705)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

99.72

100

0.997
  pilQ Acinetobacter baumannii D1279779

40.559

100

0.406
  pilQ Acinetobacter baumannii strain A118

40.559

100

0.406
  comQ Acinetobacter baylyi ADP1

41.026

98.319

0.403
  pilQ Legionella pneumophila strain Lp02

38.102

95.938

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.956

95.938

0.364