Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   R2846_RS00740 Genome accession   NC_017452
Coordinates   143825..145162 (+) Length   445 a.a.
NCBI ID   WP_014550414.1    Uniprot ID   -
Organism   Haemophilus influenzae R2846     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 138825..150162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2846_RS00715 (R2846_0140) - 138890..141484 (-) 2595 WP_014550411.1 penicillin-binding protein 1A -
  R2846_RS00720 (R2846_0141) comA 141583..142380 (+) 798 WP_013527435.1 pilus assembly protein PilM Machinery gene
  R2846_RS00725 (R2846_0142) comB 142381..142887 (+) 507 WP_014550412.1 competence protein ComB Machinery gene
  R2846_RS00730 (R2846_0143) comC 142884..143405 (+) 522 WP_005662592.1 competence protein ComC Machinery gene
  R2846_RS00735 (R2846_0144) comD 143402..143815 (+) 414 WP_014550413.1 pilus assembly protein PilP Machinery gene
  R2846_RS00740 (R2846_0145) comE 143825..145162 (+) 1338 WP_014550414.1 type IV pilus secretin PilQ family protein Machinery gene
  R2846_RS00745 (R2846_0146) - 145175..145861 (+) 687 WP_014550415.1 ComF family protein -
  R2846_RS00750 (R2846_0147) nfuA 145937..146533 (+) 597 WP_005656459.1 Fe-S biogenesis protein NfuA -
  R2846_RS00755 (R2846_0148) nudC 146600..147394 (+) 795 WP_014550416.1 NAD(+) diphosphatase -
  R2846_RS00760 (R2846_0149) - 147430..148020 (+) 591 WP_005656455.1 YjaG family protein -
  R2846_RS00765 (R2846_0150) - 148160..148432 (+) 273 WP_014550417.1 HU family DNA-binding protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49162.50 Da        Isoelectric Point: 7.8653

>NTDB_id=47288 R2846_RS00740 WP_014550414.1 143825..145162(+) (comE) [Haemophilus influenzae R2846]
MKKYLLKCGYFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTLEQLAFQQDVNLVIGDILENKISLKLNNIDMPRLLQ
IIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHLVSHTIKLHFAKASELMKSLTTGSGSLLSPAGSITFDDRSN
LLVIQDEPRFVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYVVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSAGKK

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=47288 R2846_RS00740 WP_014550414.1 143825..145162(+) (comE) [Haemophilus influenzae R2846]
ATGAAGAAATATCTTTTAAAGTGCGGTTATTTTTTAGTATGTTTTTGTTTGCCATTAATCGTTTTTGCTAATCCTAAAAC
AGATAACGAACGTTTTTTTATTCGTTTATCGCAAGCACCTTTAGCCCAAACACTGGAGCAATTAGCTTTCCAACAAGATG
TGAATTTAGTGATTGGAGATATATTGGAAAACAAGATCTCTTTGAAATTAAACAATATTGATATGCCACGTTTGCTACAA
ATAATCGCAAAAAGTAAGCATCTTACTTTGAATAAAGATGATGGGGTTTATTATTTAAACGGCAGTCAATCTGGCAAAGG
TCAGGTTGCAGGAAATCTTACGACAAATGAACCGCACTTAGTGAGTCATACGATAAAACTTCATTTTGCTAAAGCCTCTG
AATTAATGAAATCCTTAACAACAGGAAGTGGATCTTTGCTTTCTCCTGCGGGGAGCATTACCTTTGATGATCGCAGTAAT
TTGCTGGTTATTCAGGATGAACCTCGTTTTGTGCAAAATATCAAAAAACTGATTGCTGAAATGGATAAGCCTATTGAACA
GATCGCTATTGAAGCGCGAATTGTGACAATTACGGATGAGAGTTTGAAAGAACTTGGCGTTCGGTGGGGGATTTTTAATC
CAACTGAAAATGCAAGACGAGTTGCGGGCAGCCTTACAGGCAATAGCTTTGAAAATATTGCGGATAATCTTAATGTAAAT
TTTGCGACAACGACGACACCTGCTGGCTCTATAGCATTACAAGTCGCGAAAATTAATGGGCGATTGCTTGATTTAGAATT
GAGTGCGTTGGAGCGTGAAAATAATGTAGAAATTATTGCAAGTCCTCGCTTACTCACTACCAATAAGAAAAGTGCAAGCA
TTAAACAGGGGACAGAAATTCCTTACGTCGTGAGTAATACTCGTAACGATACGCAGTCTGTGGAATTTCGTGAGGCGGTG
CTTGGTTTGGAAGTGACGCCACATATTTCTAAAGATAACAATATCTTACTTGATTTATTGGTGAGTCAAAATTCCCCTGG
TTCTCGTGTCGCTTATGGACAAAATGAGGTGGTTTCTATTGATAAGCAAGAAATTAATACTCAGGTTTTTGCCAAAGATG
GGGAAACCATTGTGCTTGGCGGCGTATTTCACGACACAATCACGAAAAGCGAAGATAAAGTGCCATTGCTTGGCGATATA
CCCGTTATTAAACGATTATTTAGCAAAGAAAGTGAACGACATCAAAAACGTGAGCTCGTGATTTTCGTGACGCCACATAT
TTTAAAAGCAGGAGAAACGTTAGAGGCGTTGAAACAAAAAAGTGCGGGGAAAAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(123-187)

Bacterial type II and III secretion system protein (Secretin)

(269-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

98.652

100

0.987
  comE Haemophilus influenzae 86-028NP

97.528

100

0.975
  comE Glaesserella parasuis strain SC1401

52.706

95.506

0.503
  pilQ Vibrio campbellii strain DS40M4

42.959

94.157

0.404
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.233

92.584

0.391
  pilQ Vibrio cholerae strain A1552

42.233

92.584

0.391

Multiple sequence alignment