Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H3303_RS03225 Genome accession   NZ_CP059738
Coordinates   738331..740412 (+) Length   693 a.a.
NCBI ID   WP_175552958.1    Uniprot ID   -
Organism   Pseudomonas sp. 29A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733331..745412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3303_RS03205 (H3303_03205) pilM 735543..736607 (+) 1065 WP_016983851.1 pilus assembly protein PilM Machinery gene
  H3303_RS03210 (H3303_03210) - 736607..737173 (+) 567 WP_123449350.1 PilN domain-containing protein -
  H3303_RS03215 (H3303_03215) pilO 737170..737793 (+) 624 WP_016983853.1 type 4a pilus biogenesis protein PilO -
  H3303_RS03220 (H3303_03220) - 737790..738317 (+) 528 WP_175552957.1 pilus assembly protein PilP -
  H3303_RS03225 (H3303_03225) pilQ 738331..740412 (+) 2082 WP_175552958.1 type IV pilus secretin PilQ Machinery gene
  H3303_RS03230 (H3303_03230) aroK 740417..740935 (+) 519 WP_011332081.1 shikimate kinase AroK -
  H3303_RS03235 (H3303_03235) aroB 741088..742188 (+) 1101 WP_175552959.1 3-dehydroquinate synthase -
  H3303_RS03240 (H3303_03240) - 742199..743788 (+) 1590 WP_016983857.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75172.65 Da        Isoelectric Point: 5.5748

>NTDB_id=471680 H3303_RS03225 WP_175552958.1 738331..740412(+) (pilQ) [Pseudomonas sp. 29A]
MNRIFSTLGFSLWIALMSPMVFAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYNTRVEGNNLFVVVGQGAPAAAPRVAAVTPRAAVAAAAPAKAFVPKNRTIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFTRTQLPDKLRVRLDVKDFATPVQFVNAGVSGDRTVITVEPSG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGTSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=471680 H3303_RS03225 WP_175552958.1 738331..740412(+) (pilQ) [Pseudomonas sp. 29A]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTATTCGCGGCCAATCTGAA
GACGCTCGACGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCACCGCCACAGCCCAAGG
GCTACACCACCGAATCGCCGGCGCGGATCGCCCTGGATTTGCCCGGTGTTGCCAGCCAGTTGGCCAGCAAGAACCTTGAT
CTGGGCAGCGGCAATGCACGCACCGCCACGGTAGTCGAAGCCAAGGACCGCACGCGGCTGATCGTCAGCCTCACGCAACT
GGCGCCTTACAACACGCGGGTCGAGGGCAACAATCTGTTCGTGGTGGTCGGGCAGGGCGCGCCGGCGGCGGCGCCGAGAG
TCGCAGCCGTTACACCACGCGCTGCGGTAGCGGCTGCGGCACCGGCCAAGGCCTTTGTGCCGAAAAACCGGACCATTCGC
GGCGTGGATTTCCAGCGGGGTACGGCAGGTGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCATCGCTCCGGATAT
CCAGGAACATGACGGCAAGATCATCCTCAGCTTTACCCGCACGCAGTTACCAGACAAGTTGCGGGTACGCCTCGACGTCA
AGGATTTTGCTACTCCGGTGCAGTTCGTCAATGCCGGGGTGAGCGGTGATCGCACGGTTATTACTGTCGAACCCAGCGGC
ACCTACGAGTACTCCACCTTCCAGACCGACAACAAACTAACCGTCAGCATCCGCCCGATGACGGTCGATGACCTGCAGAA
ACGTAATGCCGACCGTCAGGCGTACGTCGGTGAAAAGCTCTCGCTGAACTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATTGCTGACTTCACCAACCTCAACCTGGTCGCCAGCGACACGGTGCAGGGCGGCATCACCTTACGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTGCTGGT
GGCTCCCGCCGATGAAATCGCTGCCCGCGAGCGTCAGGAACTGGAATCGCAGAAACAGATCGCCGAACTGGCGCCGTTGC
GCCGCGAACTGTTGCAAGTGAATTACGCCAAGGCTGCGGACATCGCCAAGTTGTTCCAGTCGGTGACCAGTGCCGAGGCT
AAAATCGACGAGCGTGGCTCGATCACCGTCGATGAGCGGACCAATAACATCATTGCCTACCAGACTCAGGATCGCCTCGA
CGAACTGCGGCGGATCGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTAATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAAAGTCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACACTTCAGGCGTGAGC
AACGGCACATCGACCACCATCGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGTACCGTGGGCAA
CACTTCCGGGATCGGTATCGCCTTCATCACCGACAACGTGTTGCTCGATCTTGAGCTGACGGCAATGGAGAAAACCGGCA
ACGGTGAAATCGTTTCGCAGCCCAAGGTCGTTACCTCGGATAAGGAAACCGCGAAAATCCTCAAGGGCACCGAGATTCCG
TATCAGGAAGCCAGTTCCAGCGGCGCGACATCGGTGTCGTTCAAGGAGGCCTCGCTGTCATTGGAAGTCACCCCGCAGAT
TACCCCAGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCA
AATACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(528-684)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.626

100

0.75
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii strain A118

37.936

100

0.408
  pilQ Acinetobacter baumannii D1279779

37.936

100

0.408
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.547

0.372
  pilQ Legionella pneumophila strain Lp02

38.166

97.547

0.372