Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H3303_RS03205 Genome accession   NZ_CP059738
Coordinates   735543..736607 (+) Length   354 a.a.
NCBI ID   WP_016983851.1    Uniprot ID   A0A7H8GED1
Organism   Pseudomonas sp. 29A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 730543..741607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3303_RS03195 (H3303_03195) - 731265..732533 (+) 1269 WP_102899524.1 malic enzyme-like NAD(P)-binding protein -
  H3303_RS03200 (H3303_03200) - 732879..735323 (-) 2445 WP_371929253.1 penicillin-binding protein 1A -
  H3303_RS03205 (H3303_03205) pilM 735543..736607 (+) 1065 WP_016983851.1 pilus assembly protein PilM Machinery gene
  H3303_RS03210 (H3303_03210) - 736607..737173 (+) 567 WP_123449350.1 PilN domain-containing protein -
  H3303_RS03215 (H3303_03215) pilO 737170..737793 (+) 624 WP_016983853.1 type 4a pilus biogenesis protein PilO -
  H3303_RS03220 (H3303_03220) - 737790..738317 (+) 528 WP_175552957.1 pilus assembly protein PilP -
  H3303_RS03225 (H3303_03225) pilQ 738331..740412 (+) 2082 WP_175552958.1 type IV pilus secretin PilQ Machinery gene
  H3303_RS03230 (H3303_03230) aroK 740417..740935 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38120.72 Da        Isoelectric Point: 4.8109

>NTDB_id=471679 H3303_RS03205 WP_016983851.1 735543..736607(+) (pilM) [Pseudomonas sp. 29A]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGLSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=471679 H3303_RS03205 WP_016983851.1 735543..736607(+) (pilM) [Pseudomonas sp. 29A]
GTGCTGGGACTCTTCAATAAAAAAGCCAATACGTTACTGGGGATCGACATCAGCTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTACGCGGTAGAGCCGTTGCCGGTCAACGCCGTGGTCGAAAAGAACA
TTGCCGAACTTGAAGGCGTCGGTCAGGCCCTGAGCCGTGTGCTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCGGTC
GCGGTGGCCGGTTCCGCCGTGATAACCAAAGTCATCGAAATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAAGCGGACCAATACATTCCCTATCCGCTGGACGAAGTCGCTATCGATTTCGAAGTCCAGGGCTTATCACCGC
GCAACCCCGAGCGGGTGAACGTGTTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCTCTC
GCCGGGCTGACCGCGCGGGTAGTCGATGTCGAGGCCTATGCACTGGAGCGCTCGTTCGGCTTGCTCGCAACGCAACTGGC
GGCCTCTCAGGAGCGTCTGACCGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGTCGGCAATTGACCGAGGAAATCCAGCGTCGTTATGGCCTGACCCTC
GAGCAGGCAGGGTTGGCGAAAAAGCAGGGCGGTCTGCCCGACGATTACATCAGCGAGGTCTTGCAGCCGTTTCGTGAGGC
GCTGGTGCAGCAGGTCTCACGTTCATTGCAGTTCTTCTTCGCTTCCGGGCAATACAACGCCGTCGACCACATTCTGCTGG
CTGGCGGCACGGCGTCGGTACCCGGGCTGGATAGGCTGATCGAGCAGCGCCTGAACACGCCGACGCAAGTGGCCAACCCG
TTTGCCAACATGGCCTTGAGCAGCAAGGTCAACGCTGGCGCGCTGGCCAGCGATGCGCCGGCGCTGATGATCGCCTGTGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8GED1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475