Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HBNCFIEN_RS05000 Genome accession   NZ_CP059576
Coordinates   1199426..1200490 (+) Length   354 a.a.
NCBI ID   WP_182392978.1    Uniprot ID   -
Organism   Legionella sp. PC997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1194426..1205490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBNCFIEN_RS04990 (HBNCFIEN_01008) - 1195627..1197996 (-) 2370 WP_182392976.1 penicillin-binding protein 1A -
  HBNCFIEN_RS04995 (HBNCFIEN_01009) - 1198167..1199183 (-) 1017 WP_182392977.1 hypothetical protein -
  HBNCFIEN_RS05000 (HBNCFIEN_01010) pilM 1199426..1200490 (+) 1065 WP_182392978.1 pilus assembly protein PilM Machinery gene
  HBNCFIEN_RS05005 (HBNCFIEN_01011) pilN 1200497..1201090 (+) 594 WP_182392979.1 PilN domain-containing protein Machinery gene
  HBNCFIEN_RS05010 (HBNCFIEN_01012) pilO 1201087..1201689 (+) 603 WP_182392980.1 type 4a pilus biogenesis protein PilO Machinery gene
  HBNCFIEN_RS05015 (HBNCFIEN_01013) pilP 1201686..1202201 (+) 516 WP_182392981.1 pilus assembly protein PilP Machinery gene
  HBNCFIEN_RS05020 (HBNCFIEN_01014) pilQ 1202210..1204315 (+) 2106 WP_182392982.1 type IV pilus secretin PilQ Machinery gene
  HBNCFIEN_RS05025 (HBNCFIEN_01015) aroK 1204636..1205163 (+) 528 WP_220471005.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38885.64 Da        Isoelectric Point: 4.5014

>NTDB_id=470897 HBNCFIEN_RS05000 WP_182392978.1 1199426..1200490(+) (pilM) [Legionella sp. PC997]
MLRLFQPKHRSILGIDITSTAVKILEISGQGDELVVENYGREVLPANAMDGNTIKDVDAVSQCIKKIIDRLNTSCKQAAL
AVPDSSIINKVIQINEGLSEAEMEELIVTEADKYIPYPIDEINLDFEILGHSEKNPNMLDVLIVASRAENVNQRVDIAVH
SGLEVTVVDVESYAVERAAQQLAKDLPASGQDKTIAIIDIGASYTHLFVLQGMKLVYSREEKFGGKQLIDSTAEHYKMTL
EQVMLAKDNGTLPEDYEKEVLEPFKEDILLQIKRTLQFFYSTSQDGDVDHILLAGGLAKLPGLVALVQERLGMSTTIANP
LSHMTPGKMVNLDSINNDAPGLMVACGLALREVK

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=470897 HBNCFIEN_RS05000 WP_182392978.1 1199426..1200490(+) (pilM) [Legionella sp. PC997]
ATGCTCAGACTGTTTCAACCCAAGCATCGTTCCATTCTCGGAATCGATATAACATCAACAGCGGTTAAAATCTTAGAAAT
TTCAGGCCAGGGCGATGAGTTGGTTGTCGAAAACTATGGCCGTGAAGTACTTCCTGCAAATGCAATGGATGGGAACACTA
TTAAAGATGTTGATGCAGTCTCTCAGTGCATAAAAAAAATAATTGACCGTCTAAATACCTCATGTAAGCAAGCTGCTCTA
GCGGTTCCTGATTCCTCAATCATTAACAAAGTAATCCAAATTAATGAGGGTTTAAGTGAGGCAGAGATGGAGGAGTTGAT
TGTTACAGAAGCAGATAAATATATTCCTTATCCGATAGATGAAATCAATCTTGACTTTGAAATTTTAGGTCACTCTGAAA
AAAATCCGAATATGCTTGACGTGTTAATTGTTGCTTCACGAGCAGAAAATGTGAATCAACGTGTAGATATTGCCGTCCAC
TCGGGTTTGGAGGTAACAGTGGTTGACGTCGAATCTTATGCCGTAGAACGCGCAGCGCAGCAACTTGCTAAAGATTTACC
TGCCTCTGGACAAGATAAAACCATTGCCATCATCGATATTGGTGCAAGTTACACGCATTTGTTTGTATTACAAGGAATGA
AATTAGTTTACTCAAGAGAAGAAAAATTTGGCGGAAAGCAATTAATAGACTCGACTGCGGAACACTATAAAATGACTTTA
GAACAAGTCATGTTGGCTAAGGATAATGGAACATTACCTGAGGATTATGAAAAGGAAGTATTAGAACCGTTTAAAGAGGA
TATTTTGTTGCAAATCAAGCGTACCTTGCAGTTTTTTTATTCCACAAGTCAGGATGGCGATGTAGATCATATTTTATTGG
CAGGAGGATTAGCCAAGTTGCCAGGTTTGGTGGCGTTGGTTCAAGAGCGATTGGGGATGAGTACGACAATTGCCAATCCA
TTGTCACATATGACTCCAGGTAAGATGGTGAATTTAGATTCTATAAATAATGATGCTCCAGGACTAATGGTAGCTTGTGG
ATTGGCTCTAAGGGAAGTTAAGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

71.186

100

0.712
  comM Acinetobacter nosocomialis M2

45.014

99.153

0.446
  pilM Acinetobacter baumannii D1279779

44.729

99.153

0.443
  comM Acinetobacter baylyi ADP1

43.305

99.153

0.429