Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HBNCFIEN_RS05020 Genome accession   NZ_CP059576
Coordinates   1202210..1204315 (+) Length   701 a.a.
NCBI ID   WP_182392982.1    Uniprot ID   -
Organism   Legionella sp. PC997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1197210..1209315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBNCFIEN_RS04995 (HBNCFIEN_01009) - 1198167..1199183 (-) 1017 WP_182392977.1 hypothetical protein -
  HBNCFIEN_RS05000 (HBNCFIEN_01010) pilM 1199426..1200490 (+) 1065 WP_182392978.1 pilus assembly protein PilM Machinery gene
  HBNCFIEN_RS05005 (HBNCFIEN_01011) pilN 1200497..1201090 (+) 594 WP_182392979.1 PilN domain-containing protein Machinery gene
  HBNCFIEN_RS05010 (HBNCFIEN_01012) pilO 1201087..1201689 (+) 603 WP_182392980.1 type 4a pilus biogenesis protein PilO Machinery gene
  HBNCFIEN_RS05015 (HBNCFIEN_01013) pilP 1201686..1202201 (+) 516 WP_182392981.1 pilus assembly protein PilP Machinery gene
  HBNCFIEN_RS05020 (HBNCFIEN_01014) pilQ 1202210..1204315 (+) 2106 WP_182392982.1 type IV pilus secretin PilQ Machinery gene
  HBNCFIEN_RS05025 (HBNCFIEN_01015) aroK 1204636..1205163 (+) 528 WP_220471005.1 shikimate kinase AroK -
  HBNCFIEN_RS05030 (HBNCFIEN_01016) aroB 1205150..1206253 (+) 1104 WP_182392983.1 3-dehydroquinate synthase -
  HBNCFIEN_RS05035 (HBNCFIEN_01017) - 1206256..1207725 (+) 1470 WP_182392984.1 SPOR domain-containing protein -
  HBNCFIEN_RS05040 (HBNCFIEN_01018) proC 1207834..1208670 (-) 837 WP_182392985.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 77479.46 Da        Isoelectric Point: 9.8261

>NTDB_id=470901 HBNCFIEN_RS05020 WP_182392982.1 1202210..1204315(+) (pilQ) [Legionella sp. PC997]
MRKIATLFILFCMSVGMTFAQNNPLISVKVIPLPEEKLRMDFEFANPIKQEPASFVTQSPARIVLDFIDSNLQLPRKQKA
NVIKLGSLNRYSVVAVGSRVRAVLELNYSVPYSGTISGRVYTVILNGKSNELFQPPQALIITNRPAKTNYVINHFDFRGI
ERQGGRAIVNVSSTSVPTEVEEHGKEILIKFLNTKVPPNLRKRFDVSDFHSPVQMVTFQQVGKNAHMTLFNKGGYGQNVY
QVNQQFMIDVIPLTPEEEQQAKLKKKVFSGKRLSLNFQNISIRAVLQLLADFTGINIVVSDQVQGDITLRLNNVPWDQAL
DIILTTQGLDKRQSGNVILVDKKGVFEKMEAEQLKNQQTIQKNEPVRSELIQINYAKATELAVLIKDKQNSLLSEKGSVS
VDTRTNTIWIQDIGGRIDEVRELIKQLDVPVKQVLIEARIVEVTKDFAQDLGIRWGVSKPTHLSGTLEGANQLAQGVAPA
NVKPFTDRLNLDLVAAPLTGATPASVGIALAQLGDNILLDLELSALESEGLAELISSPRLITANQQAAEIQSGQEIPYQE
ATSSGATAVAFKKAVLSLNVKPQITPDNKILMELKINQDRALPNLTFNGVPAIATKEIQTNVLVSNGQTIVLGGIYQQDK
SKTITRVPFFGQLPVVGNLFKNTQVGIRNDELLIFITPKIITNSLSITAIEGRKVVRFDEK

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=470901 HBNCFIEN_RS05020 WP_182392982.1 1202210..1204315(+) (pilQ) [Legionella sp. PC997]
GTGCGGAAAATAGCTACTTTATTCATTTTATTTTGCATGAGCGTGGGTATGACTTTTGCCCAGAATAATCCTTTGATATC
CGTCAAAGTAATTCCTTTGCCAGAGGAAAAGTTACGTATGGATTTCGAATTTGCAAATCCCATAAAACAAGAACCTGCAA
GTTTTGTTACTCAAAGCCCAGCACGCATAGTCCTTGATTTTATTGACTCCAATTTGCAGTTGCCTCGAAAACAAAAAGCC
AATGTAATTAAATTGGGCTCGCTGAATCGGTACTCGGTTGTTGCGGTGGGTTCACGTGTTCGGGCTGTTTTGGAGTTAAA
TTATTCAGTGCCTTATTCAGGAACCATATCTGGAAGGGTTTATACTGTTATTTTGAATGGCAAAAGTAATGAATTATTCC
AACCCCCTCAAGCACTGATAATTACCAATCGCCCAGCCAAGACAAATTATGTAATCAATCATTTTGATTTTCGAGGAATT
GAACGACAGGGTGGTCGAGCAATAGTGAATGTTTCTAGTACGAGTGTTCCCACCGAAGTGGAGGAGCATGGGAAAGAAAT
TCTAATTAAATTTTTAAATACTAAAGTTCCACCGAACTTACGGAAGCGATTCGATGTTTCGGATTTCCACAGCCCAGTGC
AAATGGTTACTTTTCAGCAAGTGGGTAAAAATGCGCATATGACTTTATTCAACAAAGGAGGCTATGGCCAGAATGTCTAT
CAGGTTAATCAACAATTTATGATTGACGTAATTCCTTTAACACCAGAAGAGGAACAACAGGCCAAATTAAAAAAGAAGGT
TTTTTCGGGGAAAAGACTTTCCTTAAATTTCCAAAACATCAGCATTCGAGCCGTATTACAGTTACTTGCTGATTTTACGG
GAATTAATATCGTAGTGAGTGATCAGGTTCAAGGTGATATTACACTAAGATTAAATAATGTTCCTTGGGATCAAGCATTA
GATATTATTTTAACTACTCAAGGACTTGACAAGCGGCAATCAGGTAATGTGATTTTAGTTGATAAAAAAGGTGTCTTTGA
AAAAATGGAGGCAGAACAACTCAAAAATCAACAAACCATCCAGAAAAATGAGCCTGTACGTTCTGAGTTAATACAAATTA
ATTATGCCAAAGCAACCGAACTCGCAGTTTTGATTAAAGACAAACAAAACTCCCTTTTATCAGAAAAAGGTAGCGTGAGT
GTTGATACACGTACCAATACCATCTGGATTCAAGATATTGGGGGCAGAATTGATGAAGTTAGAGAGTTGATTAAACAGTT
GGATGTACCTGTCAAACAAGTGCTTATTGAGGCACGGATCGTTGAAGTAACGAAAGATTTTGCTCAAGACCTTGGAATTC
GGTGGGGGGTTTCCAAACCTACTCATCTCAGTGGTACTTTAGAGGGGGCGAATCAATTAGCGCAGGGAGTTGCTCCTGCA
AACGTCAAGCCCTTTACTGACCGATTAAACCTGGATTTAGTTGCTGCCCCATTGACAGGAGCTACACCAGCTTCTGTAGG
TATTGCATTGGCACAGCTTGGCGATAATATCCTACTTGACCTTGAACTCTCTGCTTTGGAAAGTGAGGGACTTGCTGAAT
TAATATCTAGTCCTAGGCTGATTACAGCCAATCAACAGGCTGCAGAAATTCAATCGGGTCAGGAAATTCCCTATCAGGAA
GCCACTTCAAGTGGGGCAACAGCAGTAGCTTTCAAAAAGGCGGTATTAAGTTTAAATGTAAAACCCCAAATTACTCCAGA
CAACAAGATACTGATGGAATTGAAGATTAATCAGGATAGGGCCTTGCCAAACTTGACATTTAATGGGGTGCCGGCAATTG
CAACTAAAGAAATTCAGACGAATGTTCTGGTCAGTAATGGACAAACAATAGTGTTGGGTGGGATTTATCAGCAAGATAAG
AGTAAGACCATTACGAGGGTACCATTTTTTGGCCAGTTACCGGTGGTTGGAAATTTGTTCAAAAACACACAAGTGGGGAT
TCGAAATGATGAATTGCTTATTTTCATAACCCCCAAAATAATAACAAATTCACTATCAATTACGGCAATTGAGGGGAGAA
AAGTAGTTCGTTTTGATGAAAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(369-433)

Secretin and TonB N terminus short domain (STN)

(295-340)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

71.326

99.001

0.706
  pilQ Legionella pneumophila strain ERS1305867

71.182

99.001

0.705
  pilQ Pseudomonas aeruginosa PAK

37.026

97.86

0.362