Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HBNCFIEN_RS05010 Genome accession   NZ_CP059576
Coordinates   1201087..1201689 (+) Length   200 a.a.
NCBI ID   WP_182392980.1    Uniprot ID   A0A7G4REM0
Organism   Legionella sp. PC997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1196087..1206689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBNCFIEN_RS04995 (HBNCFIEN_01009) - 1198167..1199183 (-) 1017 WP_182392977.1 hypothetical protein -
  HBNCFIEN_RS05000 (HBNCFIEN_01010) pilM 1199426..1200490 (+) 1065 WP_182392978.1 pilus assembly protein PilM Machinery gene
  HBNCFIEN_RS05005 (HBNCFIEN_01011) pilN 1200497..1201090 (+) 594 WP_182392979.1 PilN domain-containing protein Machinery gene
  HBNCFIEN_RS05010 (HBNCFIEN_01012) pilO 1201087..1201689 (+) 603 WP_182392980.1 type 4a pilus biogenesis protein PilO Machinery gene
  HBNCFIEN_RS05015 (HBNCFIEN_01013) pilP 1201686..1202201 (+) 516 WP_182392981.1 pilus assembly protein PilP Machinery gene
  HBNCFIEN_RS05020 (HBNCFIEN_01014) pilQ 1202210..1204315 (+) 2106 WP_182392982.1 type IV pilus secretin PilQ Machinery gene
  HBNCFIEN_RS05025 (HBNCFIEN_01015) aroK 1204636..1205163 (+) 528 WP_220471005.1 shikimate kinase AroK -
  HBNCFIEN_RS05030 (HBNCFIEN_01016) aroB 1205150..1206253 (+) 1104 WP_182392983.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23022.88 Da        Isoelectric Point: 5.8116

>NTDB_id=470899 HBNCFIEN_RS05010 WP_182392980.1 1201087..1201689(+) (pilO) [Legionella sp. PC997]
MNGINLNELTMENLGQWPLVVKICVLAGVSILIIALGYWLIIQDNFTQYDQLQAQEVTLKTDFEGKQRQLINLPAYRQQL
QIMTERFTQMLKQLPEKNQMPGLLESISKTGVASGLRFELFAPQPEVMHDFYIELPIKISVVGTYFQLAMFISRVAEMDR
IVTLHDFSIERVSSKDKDKVVSGDELIMSITAKIYRYRTQ

Nucleotide


Download         Length: 603 bp        

>NTDB_id=470899 HBNCFIEN_RS05010 WP_182392980.1 1201087..1201689(+) (pilO) [Legionella sp. PC997]
ATGAACGGTATTAACCTGAATGAATTGACAATGGAAAATTTGGGACAGTGGCCGTTAGTGGTTAAGATTTGTGTTCTTGC
TGGGGTTAGTATTTTGATTATTGCTCTGGGGTATTGGTTAATCATACAAGATAATTTTACCCAATATGATCAACTTCAAG
CTCAGGAGGTTACATTAAAAACAGATTTTGAAGGTAAGCAACGTCAGTTAATTAATTTGCCAGCTTATCGCCAGCAACTG
CAGATTATGACGGAGCGTTTTACCCAAATGCTGAAACAGCTTCCAGAAAAAAATCAGATGCCCGGTTTATTGGAATCCAT
TTCCAAAACAGGTGTTGCATCAGGCTTAAGATTTGAGTTATTTGCGCCCCAACCTGAAGTAATGCACGATTTCTACATTG
AGTTACCTATAAAAATTTCAGTAGTAGGTACTTATTTTCAGCTCGCCATGTTTATAAGTCGTGTTGCAGAAATGGATCGT
ATTGTAACATTACATGACTTTAGTATTGAAAGGGTGTCTTCCAAAGATAAAGATAAAGTAGTTTCGGGAGATGAATTGAT
AATGAGCATAACTGCCAAAATATATCGATATCGCACACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G4REM0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

70.5

100

0.705
  comO Acinetobacter baylyi ADP1

42.708

96

0.41
  pilO Acinetobacter baumannii D1279779

40.625

96

0.39