Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H3L96_RS01970 Genome accession   NZ_CP059565
Coordinates   394188..395870 (-) Length   560 a.a.
NCBI ID   WP_009117620.1    Uniprot ID   G4CTS0
Organism   Neisseria wadsworthii strain DSM 22245     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389188..400870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L96_RS01945 (H3L96_01935) - 390479..391054 (-) 576 WP_009117615.1 hypothetical protein -
  H3L96_RS01950 (H3L96_01940) yacG 391082..391264 (-) 183 WP_009117616.1 DNA gyrase inhibitor YacG -
  H3L96_RS01955 (H3L96_01945) coaE 391414..392037 (-) 624 WP_009117617.1 dephospho-CoA kinase -
  H3L96_RS01960 (H3L96_01950) pilD 392034..392912 (-) 879 WP_009117618.1 A24 family peptidase Machinery gene
  H3L96_RS01965 (H3L96_01955) pilG 392899..394146 (-) 1248 WP_009117619.1 type II secretion system F family protein Machinery gene
  H3L96_RS01970 (H3L96_01960) pilF 394188..395870 (-) 1683 WP_009117620.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L96_RS01975 (H3L96_01965) - 396221..397318 (+) 1098 WP_182073234.1 conjugal transfer protein -
  H3L96_RS01980 (H3L96_01970) - 397341..397811 (+) 471 WP_107688794.1 low molecular weight protein-tyrosine-phosphatase -
  H3L96_RS01985 (H3L96_01975) - 397882..398406 (-) 525 WP_182073235.1 superoxide dismutase family protein -
  H3L96_RS01990 (H3L96_01980) tmk 398672..399295 (+) 624 WP_182073236.1 dTMP kinase -
  H3L96_RS01995 (H3L96_01985) - 399323..400300 (+) 978 WP_182073237.1 DNA polymerase III subunit delta' -
  H3L96_RS02000 (H3L96_01990) - 400319..400672 (+) 354 WP_107688797.1 PilZ domain-containing protein -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62083.55 Da        Isoelectric Point: 5.5890

>NTDB_id=470694 H3L96_RS01970 WP_009117620.1 394188..395870(-) (pilF) [Neisseria wadsworthii strain DSM 22245]
MSVGLLRILVQSQVISNEQAAGFQEALKKNQNKDVAPMLFEANIITPEALAGLLARVFSYPLLDLSYYPRSNVLTDILSE
EAMVQNRCLPIFRRGGKVFLAVADPTQIQTYQKIAFTAGVTFDLVVVRSDQLDSILEWQSQRSTSILKEMSMEQEIAASS
QTLYIDNEEAEDGPIPRFIHKTLSDALSVGASDIHFEFYEQVARVRFRVDGQLREVVQPPLAVRGQLASRIKVMARLDIS
EKRVPQDGRIQIAFSKHGRPIDFRVNTLPTLFGEKVVMRILNSDASTLNIDQLGFEPFQKELLLEAIHRPYGMVLVTGPT
GSGKTVSLYTCLNILNTENVNISTAEDPAEINLPGINQVNVNEKQGLTFASALKAFLRQDPDIIMVGEIRDLETADIAIK
ASQTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCSCKVPVERPPVEALKHAGFTDEDLAKDWT
LYRPVGCDRCRGKGFKGRAGVYEVMPITEEMQRVIMEGGNEVDILAMAYKEGMVDLRRAGLLKVMQGITSLEEVLASTND

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=470694 H3L96_RS01970 WP_009117620.1 394188..395870(-) (pilF) [Neisseria wadsworthii strain DSM 22245]
ATGAGTGTAGGTTTACTTCGTATTTTGGTGCAAAGCCAGGTTATTTCTAATGAACAGGCAGCGGGATTTCAAGAGGCGCT
GAAAAAAAATCAAAATAAAGATGTCGCGCCAATGTTGTTTGAAGCCAACATTATTACGCCGGAAGCTTTGGCCGGTTTGT
TGGCAAGAGTATTCAGCTATCCCTTGCTGGATTTGTCTTACTATCCCAGAAGCAATGTGCTGACCGACATTTTGTCGGAA
GAAGCAATGGTGCAAAACCGCTGTTTGCCGATTTTCAGAAGAGGCGGCAAGGTTTTCTTAGCCGTTGCCGATCCCACGCA
AATCCAAACTTATCAAAAAATCGCATTTACAGCCGGCGTAACTTTCGACTTGGTTGTGGTGCGCAGCGACCAGCTTGATT
CAATATTGGAGTGGCAAAGCCAACGATCCACTTCGATTCTGAAAGAAATGAGCATGGAGCAGGAAATCGCTGCATCCTCG
CAAACGCTCTATATCGATAATGAAGAAGCTGAAGACGGCCCGATTCCGCGATTTATCCATAAAACATTGTCCGACGCGCT
CAGTGTCGGTGCATCAGATATTCACTTTGAGTTTTACGAGCAAGTTGCCCGTGTACGTTTCCGTGTGGACGGCCAGTTGC
GTGAGGTTGTGCAACCGCCGTTGGCTGTGCGGGGGCAATTGGCCTCGCGTATTAAAGTAATGGCGCGTTTGGATATTTCC
GAAAAACGCGTCCCTCAAGACGGTCGTATTCAAATTGCCTTTTCCAAGCACGGACGTCCGATTGATTTCCGTGTGAATAC
CCTGCCTACGTTGTTTGGCGAAAAAGTGGTAATGCGTATTCTGAATTCAGATGCCAGCACCCTCAATATTGACCAACTGG
GTTTTGAACCTTTTCAAAAAGAATTACTGCTTGAAGCCATTCACCGTCCTTACGGCATGGTATTGGTAACAGGGCCGACG
GGGTCAGGTAAAACGGTTTCGCTTTATACCTGTTTGAATATTCTTAATACTGAAAATGTCAATATTTCCACTGCGGAAGA
TCCGGCAGAGATTAACCTGCCCGGTATTAACCAAGTGAACGTTAATGAAAAGCAAGGCCTTACTTTTGCTTCTGCGCTGA
AAGCATTCTTGCGGCAGGATCCGGATATCATCATGGTGGGCGAGATCCGTGACTTGGAAACCGCTGATATCGCAATTAAA
GCTTCGCAAACCGGCCATATGGTATTCTCAACCCTGCATACCAACAACGCTCCGGCTACGCTTTCGCGTTTACTTAACAT
GGGTGTGGCACCATTTAACATCGCCAGCTCGGTCAGCCTGATCATGGCCCAACGGTTGTTGCGTCGCTTATGTAGTTGCA
AAGTACCTGTGGAACGCCCTCCTGTTGAGGCATTGAAACATGCCGGCTTTACAGATGAAGATTTGGCTAAAGATTGGACA
CTATACCGTCCGGTTGGATGTGACCGCTGTCGTGGCAAAGGTTTTAAAGGCCGTGCCGGTGTATATGAAGTTATGCCGAT
TACCGAAGAAATGCAGCGTGTGATTATGGAAGGCGGTAACGAAGTCGATATTTTGGCAATGGCCTACAAAGAAGGCATGG
TAGATTTGCGCCGTGCCGGTTTGTTGAAAGTTATGCAGGGCATCACTTCTTTGGAAGAAGTGTTGGCAAGCACAAATGAT
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4CTS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.005

100

0.771
  pilB Acinetobacter baumannii D1279779

52.594

99.821

0.525
  pilB Acinetobacter baylyi ADP1

51.699

99.821

0.516
  pilB Vibrio cholerae strain A1552

46.479

100

0.471
  pilB Legionella pneumophila strain ERS1305867

50.288

93.036

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

44.991

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.262

100

0.436
  pilF Thermus thermophilus HB27

38.165

97.321

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.388

93.393

0.368