Detailed information NTG0470692

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H3L96_RS01960
• Genome accession: NZ_CP059565
• Coordinates: 392034..392912 (-)
• Length: 292 a.a.
• NCBI ID: WP_009117618.1
• Uniprot ID: G4CTR8
• Organism: Neisseria wadsworthii strain DSM 22245
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 387034..397912

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H3L96_RS01935 (H3L96_01925)	argJ	387512..388729 (-)	1218	WP_009117613.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
H3L96_RS01940 (H3L96_01930)	-	388729..390150 (-)	1422	WP_050795395.1	chloride channel protein	-
H3L96_RS01945 (H3L96_01935)	-	390479..391054 (-)	576	WP_009117615.1	hypothetical protein	-
H3L96_RS01950 (H3L96_01940)	yacG	391082..391264 (-)	183	WP_009117616.1	DNA gyrase inhibitor YacG	-
H3L96_RS01955 (H3L96_01945)	coaE	391414..392037 (-)	624	WP_009117617.1	dephospho-CoA kinase	-
H3L96_RS01960 (H3L96_01950)	pilD	392034..392912 (-)	879	WP_009117618.1	A24 family peptidase	Machinery gene
H3L96_RS01965 (H3L96_01955)	pilG	392899..394146 (-)	1248	WP_009117619.1	type II secretion system F family protein	Machinery gene
H3L96_RS01970 (H3L96_01960)	pilF	394188..395870 (-)	1683	WP_009117620.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H3L96_RS01975 (H3L96_01965)	-	396221..397318 (+)	1098	WP_182073234.1	conjugal transfer protein	-
H3L96_RS01980 (H3L96_01970)	-	397341..397811 (+)	471	WP_107688794.1	low molecular weight protein-tyrosine-phosphatase	-

Sequence

Protein

• Length: 292 a.a.
• Molecular weight: 31875.97 Da
• Isoelectric Point: 8.0980

>NTDB_id=470692 H3L96_RS01960 WP_009117618.1 392034..392912(-) (pilD) [Neisseria wadsworthii strain DSM 22245]
MLWANMVAEVYAPFVVPLAVLFGLLVGSFLNVVIYRVPVMMEKQWTLFAKEHLNIEATAEEQAPFTLAQPPSRCPKCGAG
VKPWQNIPILSYLLLKGKCGGCHAPISLRYPLVELLTGILFGVVAYQYGWSWLTLGGLVFTALLIALTFIDADTQYLPDS
LTLPLIWLGLIYNLNGEFVPLQQAVLGAIVGYTSLWLLNYIFKKIRGIDGMGGGDFKLFAAFGAWLGISSLPVIIFVAAL
VGLVAAIVMRVVNTQQQIAFGPSLAIAGWLAFVANEPLRRGVAWWLNSSGLM

Nucleotide

• Length: 879 bp

>NTDB_id=470692 H3L96_RS01960 WP_009117618.1 392034..392912(-) (pilD) [Neisseria wadsworthii strain DSM 22245]
ATGTTGTGGGCTAATATGGTGGCTGAAGTTTATGCGCCTTTCGTGGTGCCGTTGGCGGTGTTGTTCGGCTTATTGGTCGG
TAGCTTTTTAAATGTGGTGATTTACCGTGTTCCGGTAATGATGGAAAAACAGTGGACACTATTTGCTAAAGAGCATCTTA
ATATAGAAGCAACAGCAGAGGAGCAAGCACCGTTTACTTTGGCTCAACCGCCTTCACGTTGCCCCAAATGCGGGGCAGGT
GTTAAGCCTTGGCAGAATATTCCGATTCTCAGCTATCTGTTATTAAAAGGGAAATGCGGTGGTTGCCATGCGCCTATCAG
CCTACGTTACCCTTTGGTGGAGCTGCTTACAGGCATTTTATTTGGTGTTGTTGCCTATCAATACGGTTGGAGCTGGCTAA
CGCTGGGTGGCTTGGTATTCACAGCATTGTTGATTGCTTTGACTTTCATCGATGCAGATACACAATACTTGCCTGATTCT
TTAACGCTGCCTTTGATTTGGTTGGGGCTGATTTATAATCTGAACGGTGAGTTTGTGCCTTTGCAGCAAGCCGTTTTAGG
TGCAATAGTCGGCTATACCAGCTTGTGGTTGCTTAACTATATATTCAAAAAAATCCGTGGTATCGACGGTATGGGTGGTG
GTGATTTTAAATTGTTTGCTGCCTTTGGTGCGTGGTTGGGTATCAGCTCTCTGCCGGTGATTATTTTCGTTGCGGCTTTG
GTAGGTTTGGTTGCGGCAATAGTGATGCGTGTAGTCAATACGCAGCAGCAAATTGCATTTGGCCCCAGCTTAGCTATTGC
GGGGTGGCTGGCTTTTGTAGCAAACGAACCTTTGCGCAGAGGCGTTGCCTGGTGGCTTAACTCTTCAGGATTGATGTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: G4CTR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	67.025	95.548	0.64
pilD	Vibrio campbellii strain DS40M4	45.756	92.808	0.425
pilD	Vibrio cholerae strain A1552	43.463	96.918	0.421
pilD	Acinetobacter nosocomialis M2	46.743	89.384	0.418
pilD	Acinetobacter baumannii D1279779	46.743	89.384	0.418