Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2677_RS14000 Genome accession   NZ_CP059558
Coordinates   3024336..3026060 (+) Length   574 a.a.
NCBI ID   WP_125298852.1    Uniprot ID   A0AAX1MGP0
Organism   Acinetobacter junii strain YR7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3019336..3031060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2677_RS13965 (H2677_13960) nusA 3020188..3021672 (-) 1485 WP_004916840.1 transcription termination factor NusA -
  H2677_RS13970 (H2677_13965) rimP 3021710..3022234 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  H2677_RS13990 (H2677_13985) secG 3022857..3023186 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  H2677_RS13995 (H2677_13990) tpiA 3023199..3023990 (-) 792 WP_004958617.1 triose-phosphate isomerase -
  H2677_RS14000 (H2677_13995) pilB 3024336..3026060 (+) 1725 WP_125298852.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2677_RS14005 (H2677_14000) pilC 3026085..3027311 (+) 1227 WP_125298853.1 type II secretion system F family protein Machinery gene
  H2677_RS14010 (H2677_14005) pilD 3027311..3028171 (+) 861 WP_212638781.1 A24 family peptidase Machinery gene
  H2677_RS14015 (H2677_14010) coaE 3028173..3028772 (+) 600 WP_212638782.1 dephospho-CoA kinase -
  H2677_RS14020 (H2677_14015) - 3028769..3029680 (-) 912 WP_212638783.1 DMT family transporter -
  H2677_RS14025 (H2677_14020) rlmB 3029711..3030460 (-) 750 WP_212638784.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H2677_RS14030 (H2677_14025) - 3030651..3030977 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63659.75 Da        Isoelectric Point: 5.0589

>NTDB_id=470602 H2677_RS14000 WP_125298852.1 3024336..3026060(+) (pilB) [Acinetobacter junii strain YR7]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVASTDTNNTTEDDDTNHQDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPQQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=470602 H2677_RS14000 WP_125298852.1 3024336..3026060(+) (pilB) [Acinetobacter junii strain YR7]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATACAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTAAATATCGAGCCAGTGATTG
TTGAGCACAACAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTCGATTTTGGGGAAAACGACGAC
TTTGATTTAGATAATGTTGATGTTGCATCGACTGATACAAACAACACAACTGAGGATGATGATACAAACCATCAAGATGA
ATCACCTATCGTCAAATATATCAATAAACTATTGATTGATGCGATTCGTATGGGAGCCTCTGACTTACATTTTGAACCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCCGACAAATTGCAAACCCACCACTACAACTCGCAACT
CGTTTAGCGTCTCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAGAAGCGTATGCCTCAAGATGGTCGTATCAAACT
TAAACTATCTAAGACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTTGTTCTTCGTA
TTTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTATTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACAGGTCCGACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTCTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGACCCAGTCGAAATCAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACGGGCTTAACTTTCTCAGCGGCATTAAAAGCATTTTTACGTCAAGATCCTGATATCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAGGCAGCTCAAACAGGTCACATGGTGATGTCGACTCTGCA
TACTAACAGTGCTCCTGAAACACTCACACGATTACGCAATATGGGAGTTCCCTCTTTCAATATTGCAACTTCTGTTAATC
TAGTGATTGCTCAACGTTTAGCCCGCCGTCTATGCTCGGAATGCAAACAACCTATCGAGATTCCTCAGCAAAGTCTTTTA
GAAATGGGCTTCACCGAACAAGATTTAGCCAACCCTGATTTTCAAATTTTTCAACCTGTAGGCTGTGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAAGTAATGAAAGTGACACCCGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCATTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAATCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.391

100

0.815
  pilB Acinetobacter baylyi ADP1

77.043

100

0.772
  pilB Legionella pneumophila strain ERS1305867

53.371

93.031

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

49.821

97.213

0.484
  pilF Neisseria gonorrhoeae MS11

50.738

94.425

0.479
  pilB Vibrio cholerae strain A1552

49.097

96.516

0.474
  pilB Vibrio campbellii strain DS40M4

51.451

90.07

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

34.416

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.764

93.031

0.361