Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H2677_RS14010 Genome accession   NZ_CP059558
Coordinates   3027311..3028171 (+) Length   286 a.a.
NCBI ID   WP_212638781.1    Uniprot ID   A0AAX1MIQ3
Organism   Acinetobacter junii strain YR7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3022311..3033171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2677_RS13990 (H2677_13985) secG 3022857..3023186 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  H2677_RS13995 (H2677_13990) tpiA 3023199..3023990 (-) 792 WP_004958617.1 triose-phosphate isomerase -
  H2677_RS14000 (H2677_13995) pilB 3024336..3026060 (+) 1725 WP_125298852.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2677_RS14005 (H2677_14000) pilC 3026085..3027311 (+) 1227 WP_125298853.1 type II secretion system F family protein Machinery gene
  H2677_RS14010 (H2677_14005) pilD 3027311..3028171 (+) 861 WP_212638781.1 A24 family peptidase Machinery gene
  H2677_RS14015 (H2677_14010) coaE 3028173..3028772 (+) 600 WP_212638782.1 dephospho-CoA kinase -
  H2677_RS14020 (H2677_14015) - 3028769..3029680 (-) 912 WP_212638783.1 DMT family transporter -
  H2677_RS14025 (H2677_14020) rlmB 3029711..3030460 (-) 750 WP_212638784.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H2677_RS14030 (H2677_14025) - 3030651..3030977 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31976.57 Da        Isoelectric Point: 7.4720

>NTDB_id=470604 H2677_RS14010 WP_212638781.1 3027311..3028171(+) (pilD) [Acinetobacter junii strain YR7]
MQSFFLYFLENPIALYLAVGLISLCIGSFLNVVIFRTPKMMEQEWHQECQMLLHPEQPIIDQSKLTLSQPASTCPKCQTP
IKWFQNIPIISWLVLRGKCGTCQNPISIRYPLIELLTMICSLIVVAVFGATIQMLFGLTLTWALIALTFIDFDTQLLPDR
FTLPLAALGLGINTFAIYTTASAAIWGYLIGFLCLWIVYYLFKIITGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSM
VGAIIGIMLMKMRGENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=470604 H2677_RS14010 WP_212638781.1 3027311..3028171(+) (pilD) [Acinetobacter junii strain YR7]
ATGCAATCTTTTTTCCTTTACTTTTTAGAAAATCCAATTGCACTTTACCTTGCAGTTGGATTAATCAGCTTGTGCATCGG
CAGTTTTTTAAATGTTGTGATCTTTCGCACTCCAAAAATGATGGAGCAAGAATGGCATCAGGAATGCCAAATGCTTTTAC
ACCCTGAACAGCCGATCATAGATCAGAGCAAGTTAACATTAAGTCAGCCAGCTTCTACTTGTCCTAAGTGTCAAACGCCT
ATTAAATGGTTCCAGAATATTCCTATTATTAGTTGGTTAGTACTTCGCGGTAAATGTGGCACTTGCCAAAATCCAATCAG
CATTCGCTACCCGTTGATTGAACTACTTACTATGATCTGTTCATTGATCGTTGTGGCAGTATTCGGTGCAACAATTCAGA
TGCTGTTCGGCTTAACTCTAACGTGGGCACTTATTGCACTCACTTTTATTGATTTTGATACACAGTTACTACCTGATCGA
TTTACGCTACCTTTAGCAGCATTAGGGCTGGGTATCAATACTTTTGCAATCTATACAACGGCTAGTGCTGCGATCTGGGG
CTATTTAATCGGTTTTCTATGTCTTTGGATTGTGTATTACCTATTTAAAATCATCACGGGCAAAGAAGGCATGGGCTATG
GTGATTTTAAACTGCTTGCAGCCCTTGGTGCATGGATGGGTCCGATGATGTTACCCCTCATCGTATTACTCTCTTCTATG
GTAGGCGCAATTATTGGTATTATGCTGATGAAAATGCGCGGTGAAAATCAACCTTTTGCATTTGGTCCTTATATTGCAAT
TGCTGGTTGGATTGCCTTTCTATGGGGTGAGCAAATTATGAAAGTATATCTTGGGGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

81.818

100

0.818
  pilD Acinetobacter nosocomialis M2

81.818

100

0.818
  pilD Vibrio cholerae strain A1552

51.389

100

0.517
  pilD Vibrio campbellii strain DS40M4

51.504

93.007

0.479
  pilD Neisseria gonorrhoeae MS11

46.154

95.455

0.441