Detailed information NTG0470522

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: H2788_RS01650
• Genome accession: NZ_CP059550
• Coordinates: 348795..349853 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain E-022166
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 343795..354853

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2788_RS01635 (H2788_01635)	-	344901..345233 (+)	333	WP_077168921.1	hypothetical protein	-
H2788_RS01640 (H2788_01640)	-	345236..346060 (+)	825	WP_347016888.1	putative RNA methyltransferase	-
H2788_RS01645 (H2788_01645)	ponA	346077..348632 (-)	2556	WP_347016890.1	penicillin-binding protein PBP1a	-
H2788_RS01650 (H2788_01650)	pilM	348795..349853 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
H2788_RS01655 (H2788_01655)	pilN	349853..350497 (+)	645	WP_151682783.1	PilN domain-containing protein	Machinery gene
H2788_RS01660 (H2788_01660)	pilO	350494..351234 (+)	741	WP_151682782.1	type 4a pilus biogenesis protein PilO	Machinery gene
H2788_RS01665 (H2788_01665)	pilP	351245..351772 (+)	528	WP_151682781.1	pilus assembly protein PilP	Machinery gene
H2788_RS01670 (H2788_01670)	pilQ	351834..353945 (+)	2112	WP_174735561.1	type IV pilus secretin PilQ family protein	Machinery gene
H2788_RS01675 (H2788_01675)	aroK	353957..354526 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=470522 H2788_RS01650 WP_004705187.1 348795..349853(+) (pilM) [Acinetobacter seifertii strain E-022166]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=470522 H2788_RS01650 WP_004705187.1 348795..349853(+) (pilM) [Acinetobacter seifertii strain E-022166]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAATTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGGCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTAGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGTCATACCATGACAACCTTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGTGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGCTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCTGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426