Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2789_RS16670 Genome accession   NZ_CP059549
Coordinates   3534664..3536364 (+) Length   566 a.a.
NCBI ID   WP_347019008.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain NCIMB13260     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3529664..3541364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2789_RS16635 (H2789_16615) nusA 3530550..3532034 (-) 1485 WP_347019006.1 transcription termination factor NusA -
  H2789_RS16640 (H2789_16620) rimP 3532072..3532596 (-) 525 WP_003654583.1 ribosome maturation factor RimP -
  H2789_RS16660 (H2789_16640) secG 3533236..3533565 (-) 330 WP_092710024.1 preprotein translocase subunit SecG -
  H2789_RS16665 (H2789_16645) tpiA 3533578..3534372 (-) 795 WP_347019007.1 triose-phosphate isomerase -
  H2789_RS16670 (H2789_16650) pilB 3534664..3536364 (+) 1701 WP_347019008.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2789_RS16675 (H2789_16655) pilC 3536392..3537618 (+) 1227 WP_004640845.1 type II secretion system F family protein Machinery gene
  H2789_RS16680 (H2789_16660) pilD 3537618..3538478 (+) 861 WP_004640843.1 A24 family peptidase Machinery gene
  H2789_RS16685 (H2789_16665) coaE 3538480..3539076 (+) 597 WP_347019009.1 dephospho-CoA kinase -
  H2789_RS16690 (H2789_16670) - 3539073..3539987 (-) 915 WP_347019010.1 DMT family transporter -
  H2789_RS16695 (H2789_16675) rlmB 3540022..3540771 (-) 750 WP_005037200.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H2789_RS16700 (H2789_16680) - 3540874..3541200 (-) 327 WP_003654567.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 63146.48 Da        Isoelectric Point: 5.3317

>NTDB_id=470509 H2789_RS16670 WP_347019008.1 3534664..3536364(+) (pilB) [Acinetobacter calcoaceticus strain NCIMB13260]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLVQRGQILYVATSNPSNIEAIDAIRFNSKLMVEQVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDISTAPEEDDDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLASRLKV
MSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKPQGML
LITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIRDLET
AEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKVPTDIPKQSLLEMGFTEQD
LTHHDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRRSGLKKVMQGVTSLQEV
NRVTNE

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=470509 H2789_RS16670 WP_347019008.1 3534664..3536364(+) (pilB) [Acinetobacter calcoaceticus strain NCIMB13260]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGGTCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGTAACCCAAGCAATATTGAAGCTATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAGCAGGTTATTG
TCGAGCATCATAAACTTGAGAAAATTCTGGCGCAGCAATTTACTGAAGAAAGTAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATATCTCAACCGCTCCAGAAGAGGATGATGACACTCCTCAGGGCGATGAAGCACCTATTGTTAAATATGTTAA
CAAGTTATTAATAGATGCGATTCGTATGGGAGCTTCAGACTTGCATTTCGAACCCTATGAGAAATCGTATCGGGTCCGTT
ATCGGGTCGATGGCGTTTTGCGTCAGATTGCAAATCCGCCTTTGCAACTAGCCAATCGTTTGGCTTCACGTTTAAAAGTT
ATGTCTCAAATGGACATTTCCGAAAAGCGTGTTCCTCAAGATGGTCGTATCAAACTCAAGCTATCTAAATCTAAAGCAAT
TGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCATCGAGTGCCATGC
TGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCACTCTTTATGGAAGCATTAGATAAGCCGCAAGGTATGCTT
TTAATTACTGGTCCTACAGGTTCTGGTAAAACAGTATCCTTATATACGGGCCTCAACATTTTAAATACTGAAAGCTCCAA
TATTTCTACTGCCGAAGATCCGGTCGAAATTAACCTTGAAGGTATTAATCAGGTTAACGTGAACCCTAAAGTAGGTCTTA
CTTTTTCGGCTGCGCTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGTGATTTAGAAACA
GCTGAAATCGCGATTAAGGCGGCTCAGACGGGTCACATGGTAATGTCCACACTGCATACCAACAGCGCCCCTGAAACACT
TACTCGTCTACGCAATATGGGAGTTCCATCTTTCAATATTGCAACATCGGTCAATCTGGTCATTGCTCAGCGTTTGGCAC
GTCGCCTATGTTCTCAATGTAAAGTACCTACTGACATTCCAAAACAAAGCCTATTGGAAATGGGTTTTACCGAACAAGAT
CTAACACACCACGATTTTCAAGTTTTTCAACCAGTCGGTTGTCCTGAATGTCGTGAGGGTTACAAAGGTCGAGTTGGTAT
TTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAATCGCTGCTGCTT
CTGAAAAATTGGGGTTTAGTAATCTACGTCGCTCAGGTTTAAAGAAAGTTATGCAAGGTGTTACTTCTTTGCAAGAAGTC
AACCGCGTAACCAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

93.333

100

0.94
  pilB Acinetobacter baylyi ADP1

78.246

100

0.788
  pilB Legionella pneumophila strain ERS1305867

52.381

100

0.525
  pilF Neisseria gonorrhoeae MS11

49.462

98.587

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

50.758

93.286

0.474
  pilB Vibrio cholerae strain A1552

49.524

92.756

0.459
  pilB Vibrio campbellii strain DS40M4

50.29

91.343

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

35.016

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.71

93.11

0.36
  pilF Thermus thermophilus HB27

36.494

98.763

0.36