Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2784_RS16070 Genome accession   NZ_CP059547
Coordinates   3453713..3455425 (+) Length   570 a.a.
NCBI ID   WP_001274987.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 10_3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3448713..3460425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2784_RS16035 (H2784_16035) nusA 3449598..3451082 (-) 1485 WP_000532248.1 transcription termination factor NusA -
  H2784_RS16040 (H2784_16040) rimP 3451120..3451644 (-) 525 WP_000777730.1 ribosome maturation factor RimP -
  H2784_RS16060 (H2784_16060) secG 3452286..3452615 (-) 330 WP_000555570.1 preprotein translocase subunit SecG -
  H2784_RS16065 (H2784_16065) tpiA 3452628..3453422 (-) 795 WP_032060899.1 triose-phosphate isomerase -
  H2784_RS16070 (H2784_16070) pilB 3453713..3455425 (+) 1713 WP_001274987.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2784_RS16075 (H2784_16075) pilC 3455455..3456681 (+) 1227 WP_000279218.1 type II secretion system F family protein Machinery gene
  H2784_RS17895 (H2784_16080) pilD 3456681..3457541 (+) 861 WP_086442553.1 prepilin peptidase Machinery gene
  H2784_RS16085 (H2784_16085) coaE 3457543..3458139 (+) 597 WP_000874125.1 dephospho-CoA kinase -
  H2784_RS16090 (H2784_16090) - 3458136..3459050 (-) 915 WP_000075015.1 DMT family transporter -
  H2784_RS16095 (H2784_16095) rlmB 3459086..3459835 (-) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H2784_RS16100 (H2784_16100) - 3459937..3460263 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63355.71 Da        Isoelectric Point: 5.2391

>NTDB_id=470419 H2784_RS16070 WP_001274987.1 3453713..3455425(+) (pilB) [Acinetobacter baumannii strain 10_3]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=470419 H2784_RS16070 WP_001274987.1 3453713..3455425(+) (pilB) [Acinetobacter baumannii strain 10_3]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACCTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGTCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCAATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGCGTGTTACGCCAAATTGCTAATCCCCCTCTACAGCTTGCAAACCGCTTAGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGTGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AAGCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGGATTAATCAAGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.825

100

0.998
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365