Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   pbN1_RS13940 Genome accession   NZ_CP059467
Coordinates   2978784..2980502 (-) Length   572 a.a.
NCBI ID   WP_169203339.1    Uniprot ID   -
Organism   Aromatoleum bremense strain PbN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2973784..2985502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  pbN1_RS13920 (pbN1_28420) - 2975570..2976196 (-) 627 WP_210147510.1 DUF502 domain-containing protein -
  pbN1_RS13925 (pbN1_28430) - 2976212..2976478 (-) 267 WP_169203342.1 zinc ribbon domain-containing protein -
  pbN1_RS13930 (pbN1_28440) pilD 2976614..2977465 (-) 852 WP_169203341.1 A24 family peptidase Machinery gene
  pbN1_RS13935 (pbN1_28450) pilC 2977532..2978767 (-) 1236 WP_169203340.1 type II secretion system F family protein Machinery gene
  pbN1_RS13940 (pbN1_28460) pilB 2978784..2980502 (-) 1719 WP_169203339.1 type IV-A pilus assembly ATPase PilB Machinery gene
  pbN1_RS13945 (pbN1_28470) ccsA 2980619..2981443 (-) 825 WP_169203338.1 cytochrome c biogenesis protein CcsA -
  pbN1_RS13950 (pbN1_28480) ffh 2981486..2982844 (+) 1359 WP_169203337.1 signal recognition particle protein -
  pbN1_RS13955 (pbN1_28490) - 2982861..2983625 (+) 765 WP_169203336.1 EI24 domain-containing protein -
  pbN1_RS13960 (pbN1_28500) - 2983636..2984385 (+) 750 WP_169203335.1 molybdopterin-binding protein -
  pbN1_RS13965 (pbN1_28510) - 2984433..2985158 (+) 726 WP_244857262.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62172.43 Da        Isoelectric Point: 5.3260

>NTDB_id=469768 pbN1_RS13940 WP_169203339.1 2978784..2980502(-) (pilB) [Aromatoleum bremense strain PbN1]
MAAAASPAALSGLARAMIQHGRLSEADAMSCTAGAASVPNGFILELAQRKLMTNRAVAWFAAETFGYPLLDIAAVDSASI
PRDAIDRKLMSKHQIVALGKRQNRLTVAAADPSNMRALDEIRFQTGLQVDLVIVEADRLARLAESLAETAEQTLRELTGE
EFDMDLLQQQEGPGEASSEDTQEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGVLREIAQPPLILKEK
IAARIKVISRLDISEKRVPQDGRMKLVLSKNKSIDFRVSSLPTLHGEKIVMRILDASSAMLGVDALGYEPEQKKILLDAI
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLSGINQVNVNDKAGLTFAASLRAFLRQDPDIIMVG
EIRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCTCKQPVDIPIEALLQA
GFPADELDGSWQPYGPVGCERCKGSGYKGRLGIYQVMPITDEIAHIIMTNGNSMDIAAQAQREGVKDLRRSGLLKVRQGV
TSLEEVLASTND

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=469768 pbN1_RS13940 WP_169203339.1 2978784..2980502(-) (pilB) [Aromatoleum bremense strain PbN1]
ATGGCAGCAGCAGCCTCCCCCGCCGCGCTCAGCGGCCTCGCACGAGCGATGATCCAGCACGGCCGACTGTCCGAAGCCGA
CGCCATGTCGTGCACGGCAGGCGCTGCCAGCGTGCCGAACGGATTTATCCTCGAGCTTGCCCAGCGCAAACTGATGACGA
ACCGGGCGGTCGCCTGGTTCGCCGCCGAAACATTCGGTTATCCGCTGCTCGACATCGCAGCCGTCGACAGCGCGAGCATT
CCCCGCGATGCGATCGACCGCAAGCTGATGAGCAAGCACCAGATCGTCGCACTCGGCAAGCGGCAGAACCGGCTGACGGT
CGCCGCCGCCGACCCGTCGAACATGCGCGCGCTCGACGAGATCCGTTTCCAGACCGGACTTCAGGTGGACCTCGTCATCG
TCGAAGCGGACCGGCTCGCGCGCCTGGCCGAGTCCCTCGCCGAAACGGCCGAACAGACGCTGAGGGAGCTCACCGGCGAA
GAGTTCGACATGGATCTCCTGCAGCAGCAGGAAGGTCCCGGTGAAGCGTCGAGCGAGGACACGCAGGAAGTCGATGACGC
GCCGGTCGTCAAGTTCATTCAGAAAGTGCTGATCGACGCGATCAACGAAGGCGCTTCCGACATCCACTTCGAGCCCTACG
AGAAGTATTACCGCATCCGCGTGCGCACCGACGGCGTGCTGCGCGAAATCGCCCAGCCACCGCTGATCCTGAAGGAAAAG
ATCGCCGCCCGGATCAAGGTCATCTCGCGCCTCGACATCTCGGAAAAGCGCGTCCCCCAGGACGGCCGGATGAAGCTCGT
GCTGTCGAAGAACAAGTCGATCGACTTTCGCGTCTCGTCGCTGCCGACGCTGCATGGCGAAAAGATCGTGATGCGGATTC
TCGACGCCTCGTCGGCGATGCTCGGCGTCGATGCGCTGGGCTACGAACCGGAACAGAAGAAAATCCTGCTCGACGCGATC
GAGCGGCCCTACGGCATGATCCTCGTGACCGGGCCGACAGGTTCCGGCAAGACGGTGTCGCTCTATACCTGCCTGAACCT
GCTCAACAAGGCCGGCGTGAATATCTCCACGGCCGAAGACCCGGCTGAAATCAATTTGTCCGGGATCAACCAGGTCAACG
TCAACGACAAGGCCGGGCTGACTTTCGCCGCATCGCTGCGGGCCTTTCTGCGGCAGGATCCGGACATCATCATGGTCGGC
GAAATCCGCGACCTTGAAACCGCCGAGATTTCGATCAAGGCCGCACAGACCGGCCACCTGGTGCTGTCGACGCTGCACAC
CAATGACGCGCCAACGACGCTCGAGCGCCTGAAAAACATGGGCGTGGCGCCATTCAACATCGCCTCGTCGGTGATCCTGA
TCACCGCCCAACGGCTTGCGCGACGCCTGTGCACGTGCAAGCAGCCGGTCGACATCCCGATCGAAGCGCTGCTGCAGGCG
GGTTTTCCCGCCGACGAACTCGACGGCAGCTGGCAGCCGTACGGCCCGGTGGGGTGCGAGCGCTGCAAGGGCAGCGGCTA
CAAGGGGCGGCTCGGGATCTACCAGGTCATGCCGATCACCGACGAGATCGCGCACATCATCATGACCAACGGCAACTCGA
TGGACATCGCGGCGCAGGCCCAGCGCGAAGGCGTCAAGGATCTGCGCCGGTCCGGACTGCTGAAGGTCAGGCAGGGCGTC
ACATCGCTCGAAGAAGTACTCGCTTCCACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.81

99.301

0.554
  pilB Acinetobacter baumannii D1279779

55.009

99.476

0.547
  pilB Legionella pneumophila strain ERS1305867

51.119

100

0.519
  pilF Neisseria gonorrhoeae MS11

55.81

91.783

0.512
  pilB Vibrio cholerae strain A1552

48.825

96.678

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

48.479

91.958

0.446
  pilB Vibrio campbellii strain DS40M4

49.116

88.986

0.437
  pilF Thermus thermophilus HB27

40.037

93.881

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

92.832

0.369