Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   I6J80_RS09605 Genome accession   NZ_CP069571
Coordinates   1895194..1896687 (+) Length   497 a.a.
NCBI ID   WP_001598631.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1250     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1890194..1901687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J80_RS09585 (I6J80_09585) pilD 1890842..1891651 (+) 810 WP_001296399.1 prepilin peptidase PppA Machinery gene
  I6J80_RS09590 (I6J80_09590) gspS2 1891717..1892127 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  I6J80_RS09595 (I6J80_09595) gspC 1892145..1893104 (+) 960 WP_204157007.1 type II secretion system protein GspC -
  I6J80_RS09600 (I6J80_09600) gspD 1893134..1895194 (+) 2061 WP_001468189.1 type II secretion system secretin GspD -
  I6J80_RS09605 (I6J80_09605) pilB/pilB1 1895194..1896687 (+) 1494 WP_001598631.1 type II secretion system ATPase GspE Machinery gene
  I6J80_RS09610 (I6J80_09610) gspF 1896687..1897910 (+) 1224 WP_001598630.1 type II secretion system inner membrane protein GspF -
  I6J80_RS09615 (I6J80_09615) gspG 1897927..1898382 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  I6J80_RS09620 (I6J80_09620) gspH 1898386..1898949 (+) 564 WP_001115117.1 type II secretion system minor pseudopilin GspH -
  I6J80_RS09625 (I6J80_09625) gspI 1898946..1899317 (+) 372 WP_000820119.1 type II secretion system minor pseudopilin GspI -
  I6J80_RS09630 (I6J80_09630) gspJ 1899314..1899919 (+) 606 WP_163435670.1 type II secretion system minor pseudopilin GspJ -
  I6J80_RS09635 (I6J80_09635) gspK 1899916..1900893 (+) 978 WP_163435669.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55204.31 Da        Isoelectric Point: 6.0089

>NTDB_id=468409 I6J80_RS09605 WP_001598631.1 1895194..1896687(+) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1250]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=468409 I6J80_RS09605 WP_001598631.1 1895194..1896687(+) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1250]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCCTCC
GAGGCTCGCCAGTTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGTGCTT
CGGATATCCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTTTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTATGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTACAGGCATCGCTTACAGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362