Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H0529_RS14660 Genome accession   NZ_CP059300
Coordinates   3017014..3018726 (+) Length   570 a.a.
NCBI ID   WP_031966873.1    Uniprot ID   A0A505MH52
Organism   Acinetobacter baumannii strain AC1633     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3012014..3023726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0529_RS14625 (H0529_14625) nusA 3012899..3014383 (-) 1485 WP_000532248.1 transcription termination factor NusA -
  H0529_RS14630 (H0529_14630) rimP 3014421..3014945 (-) 525 WP_000777730.1 ribosome maturation factor RimP -
  H0529_RS14650 (H0529_14650) secG 3015587..3015916 (-) 330 WP_000555570.1 preprotein translocase subunit SecG -
  H0529_RS14655 (H0529_14655) tpiA 3015929..3016723 (-) 795 WP_000016939.1 triose-phosphate isomerase -
  H0529_RS14660 (H0529_14660) pilB 3017014..3018726 (+) 1713 WP_031966873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H0529_RS14665 (H0529_14665) pilC 3018756..3019982 (+) 1227 WP_000279218.1 type II secretion system F family protein Machinery gene
  H0529_RS22305 (H0529_14670) pilD 3019982..3020842 (+) 861 WP_031966875.1 A24 family peptidase Machinery gene
  H0529_RS14675 (H0529_14675) coaE 3020844..3021440 (+) 597 WP_000874125.1 dephospho-CoA kinase -
  H0529_RS14680 (H0529_14680) - 3021437..3022351 (-) 915 WP_000075020.1 DMT family transporter -
  H0529_RS14685 (H0529_14685) rlmB 3022387..3023136 (-) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H0529_RS14690 (H0529_14690) - 3023238..3023564 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63335.72 Da        Isoelectric Point: 5.2391

>NTDB_id=468409 H0529_RS14660 WP_031966873.1 3017014..3018726(+) (pilB) [Acinetobacter baumannii strain AC1633]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEL
DLDVNLDGPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=468409 H0529_RS14660 WP_031966873.1 3017014..3018726(+) (pilB) [Acinetobacter baumannii strain AC1633]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCAAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCACCTCTCCC
CTTCGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACTTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGCTT
GATCTCGATGTTAATCTTGATGGTCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCCCCTTTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGTCGTATCAAGCTCAAGCTCTCAAA
AACCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTCATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCCACTGCCGAAGACCCAGTAGAAATTAACCTTGAAGGAATTAATCAAGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGTAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAACCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A505MH52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.474

100

0.995
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.617

99.474

0.504
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.887

92.982

0.482
  pilB Vibrio campbellii strain DS40M4

51.06

91.053

0.465
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

36.655

98.596

0.361