Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H0I86_RS02265 Genome accession   NZ_CP059162
Coordinates   506298..508370 (+) Length   690 a.a.
NCBI ID   WP_180923825.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain YS05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501298..513370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0I86_RS02245 (H0I86_02245) pilM 503528..504580 (+) 1053 WP_180923818.1 pilus assembly protein PilM Machinery gene
  H0I86_RS02250 (H0I86_02250) - 504580..505146 (+) 567 WP_180923820.1 PilN domain-containing protein -
  H0I86_RS02255 (H0I86_02255) - 505143..505760 (+) 618 WP_180923821.1 type 4a pilus biogenesis protein PilO -
  H0I86_RS02260 (H0I86_02260) - 505757..506284 (+) 528 WP_180923823.1 pilus assembly protein PilP -
  H0I86_RS02265 (H0I86_02265) pilQ 506298..508370 (+) 2073 WP_180923825.1 type IV pilus secretin PilQ Machinery gene
  H0I86_RS02270 (H0I86_02270) aroK 508375..508893 (+) 519 WP_180923827.1 shikimate kinase AroK -
  H0I86_RS02275 (H0I86_02275) aroB 509042..510142 (+) 1101 WP_180923829.1 3-dehydroquinate synthase -
  H0I86_RS02280 (H0I86_02280) - 510153..511742 (+) 1590 WP_180923831.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74798.92 Da        Isoelectric Point: 5.4404

>NTDB_id=467593 H0I86_RS02265 WP_180923825.1 506298..508370(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain YS05]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSRLENKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRIAGNQLFVVIGQGATAAQSAPGQPAVASGRTKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNTERPAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=467593 H0I86_RS02265 WP_180923825.1 506298..508370(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain YS05]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGACGGGCCGGTGCCCGAGCCGCGGG
GTTACACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGTCGGCTGGAGAACAAGAGCCGGGAT
CTGGGCAATGGTAATGCGCGCAGCGTGACGGTGGTCGAATCAGGGCAGCGTACCCGAGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGCACCCGAATCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCGC
CGGGGCAGCCGGCTGTCGCGTCCGGGCGGACCAAGGCCGCGGCTTCGGCGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGTGAGGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAGCGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACCC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGTACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGCGTCAGGCCAATGACTTCGGGCGATCTGCAGAAACGCAACACCGAGCG
CCCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGTGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTTGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTAGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCGGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGACCTCGAGCCGCTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGT
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGATGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTTGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATTGGTACGCCGGGTAGCACCAGCAGCAATTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACTGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACCTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATTACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAATGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAAGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

39.781

100

0.42
  pilQ Acinetobacter baumannii D1279779

37.987

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.987

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37