Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H0I86_RS02245 Genome accession   NZ_CP059162
Coordinates   503528..504580 (+) Length   350 a.a.
NCBI ID   WP_180923818.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain YS05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498528..509580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0I86_RS02235 (H0I86_02235) - 499488..500756 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  H0I86_RS02240 (H0I86_02240) - 500876..503311 (-) 2436 WP_180925779.1 penicillin-binding protein 1A -
  H0I86_RS02245 (H0I86_02245) pilM 503528..504580 (+) 1053 WP_180923818.1 pilus assembly protein PilM Machinery gene
  H0I86_RS02250 (H0I86_02250) - 504580..505146 (+) 567 WP_180923820.1 PilN domain-containing protein -
  H0I86_RS02255 (H0I86_02255) - 505143..505760 (+) 618 WP_180923821.1 type 4a pilus biogenesis protein PilO -
  H0I86_RS02260 (H0I86_02260) - 505757..506284 (+) 528 WP_180923823.1 pilus assembly protein PilP -
  H0I86_RS02265 (H0I86_02265) pilQ 506298..508370 (+) 2073 WP_180923825.1 type IV pilus secretin PilQ Machinery gene
  H0I86_RS02270 (H0I86_02270) aroK 508375..508893 (+) 519 WP_180923827.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37105.54 Da        Isoelectric Point: 4.9083

>NTDB_id=467592 H0I86_RS02245 WP_180923818.1 503528..504580(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain YS05]
MLGLFSKKASSLLGIDISSTSVKLLELSRVDGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=467592 H0I86_RS02245 WP_180923818.1 503528..504580(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain YS05]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGTGTCGACGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGTTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCAGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGTGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTGCAACA
GGTTTCGCGTTCGCTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGTATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCTTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.557

100

0.529
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457