Detailed information NTG0467104

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: H0S57_RS15425
• Genome accession: NZ_CP059080
• Coordinates: 3178858..3179916 (-)
• Length: 352 a.a.
• NCBI ID: WP_004978085.1
• Uniprot ID: N9D0Y2
• Organism: Acinetobacter johnsonii strain E10B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3173858..3184916

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0S57_RS15400 (H0S57_15405)	-	3174287..3174769 (-)	483	WP_265088291.1	shikimate kinase	-
H0S57_RS15405 (H0S57_15410)	pilQ	3174858..3176936 (-)	2079	WP_195728318.1	type IV pilus secretin PilQ family protein	Machinery gene
H0S57_RS15410 (H0S57_15415)	pilP	3176956..3177483 (-)	528	WP_195728319.1	pilus assembly protein PilP	Machinery gene
H0S57_RS15415 (H0S57_15420)	pilO	3177483..3178205 (-)	723	WP_004691497.1	type 4a pilus biogenesis protein PilO	Machinery gene
H0S57_RS15420 (H0S57_15425)	pilN	3178202..3178858 (-)	657	WP_005401354.1	PilN domain-containing protein	Machinery gene
H0S57_RS15425 (H0S57_15430)	pilM	3178858..3179916 (-)	1059	WP_004978085.1	pilus assembly protein PilM	Machinery gene
H0S57_RS15430 (H0S57_15435)	ponA	3180082..3182610 (+)	2529	WP_195728320.1	penicillin-binding protein PBP1a	-
H0S57_RS15435 (H0S57_15440)	-	3182642..3183448 (-)	807	WP_195728321.1	putative RNA methyltransferase	-
H0S57_RS15440 (H0S57_15445)	mraY	3183519..3184637 (-)	1119	WP_004691502.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38937.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=467104 H0S57_RS15425 WP_004978085.1 3178858..3179916(-) (pilM) [Acinetobacter johnsonii strain E10B]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARAEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=467104 H0S57_RS15425 WP_004978085.1 3178858..3179916(-) (pilM) [Acinetobacter johnsonii strain E10B]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCACTTGTCCCTTTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCAGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACTTCCATGGTGATTACTAAAATCATTGAAATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTAGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCTAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCACGCGCCGAAGCATTGGAGTTG
GGCGGTTTAACGCCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTTTTTTCAGATACGCTTCC
AATGGGCGTAAATACTGTTGGGATTCTGGACATCGGTCACACCATGACCACCTTGTCCGTCATGCAAAATAATAAAGTCA
TTTATACCCGTGAACAGGTGTTCGGGGGTAAACAACTGACGCAAGAAATTCAAAATCGTTATGGTTTGTCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACCCTGCCTGACGACTATGATATTGAAGTCCTTGAACCTTTTCTTGAAGCTGTGGT
GCAACAAGCAGCACGCTCTTTGCAGTTCTTCTTTTCGTCCTCTCAATTTAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTACCGGGTCACCATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9D0Y2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412