Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H0S57_RS15415 Genome accession   NZ_CP059080
Coordinates   3177483..3178205 (-) Length   240 a.a.
NCBI ID   WP_004691497.1    Uniprot ID   A0A380U8X3
Organism   Acinetobacter johnsonii strain E10B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3172483..3183205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S57_RS15395 (H0S57_15400) aroB 3173171..3174256 (-) 1086 WP_195728317.1 3-dehydroquinate synthase -
  H0S57_RS15400 (H0S57_15405) - 3174287..3174769 (-) 483 WP_265088291.1 shikimate kinase -
  H0S57_RS15405 (H0S57_15410) pilQ 3174858..3176936 (-) 2079 WP_195728318.1 type IV pilus secretin PilQ family protein Machinery gene
  H0S57_RS15410 (H0S57_15415) pilP 3176956..3177483 (-) 528 WP_195728319.1 pilus assembly protein PilP Machinery gene
  H0S57_RS15415 (H0S57_15420) pilO 3177483..3178205 (-) 723 WP_004691497.1 type 4a pilus biogenesis protein PilO Machinery gene
  H0S57_RS15420 (H0S57_15425) pilN 3178202..3178858 (-) 657 WP_005401354.1 PilN domain-containing protein Machinery gene
  H0S57_RS15425 (H0S57_15430) pilM 3178858..3179916 (-) 1059 WP_004978085.1 pilus assembly protein PilM Machinery gene
  H0S57_RS15430 (H0S57_15435) ponA 3180082..3182610 (+) 2529 WP_195728320.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27004.43 Da        Isoelectric Point: 4.9937

>NTDB_id=467102 H0S57_RS15415 WP_004691497.1 3177483..3178205(-) (pilO) [Acinetobacter johnsonii strain E10B]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAAASVENTAKADAGGAQ

Nucleotide


Download         Length: 723 bp        

>NTDB_id=467102 H0S57_RS15415 WP_004691497.1 3177483..3178205(-) (pilO) [Acinetobacter johnsonii strain E10B]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACCAAGAAAAAAATGACGGTTGATAAGTTTTT
TCAACAGTTCAATACTTTGGATCCCAATAATTACGGCAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTCTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAAGCCCAATTGCAAGAAAT
GGAAGCTAATTTCAATCAGCAGTTAGAACAGTTGCCGAAAGAAACTGAAATTCCGGGTTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAGCCAGAAATTCGCCAAGAGTTCTTTATCGAACAACCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTGCCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAATAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAGG
CTAAAACTTACCGTTATGTCGGGGCGACAGCTGCTGCAAGTGTGGAAAATACAGCTAAAGCAGATGCAGGAGGTGCTCAG
TAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380U8X3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

72.385

99.583

0.721
  comO Acinetobacter baylyi ADP1

67.755

100

0.692