Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J84_RS05285 Genome accession   NZ_CP069453
Coordinates   1109988..1110665 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain FDAARGOS_1254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1104988..1115665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J84_RS05250 (I6J84_05250) gspG 1105468..1105905 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  I6J84_RS05255 (I6J84_05255) gspH 1105913..1106422 (+) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  I6J84_RS05260 (I6J84_05260) gspI 1106419..1106796 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  I6J84_RS05265 (I6J84_05265) gspJ 1106789..1107376 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  I6J84_RS05270 (I6J84_05270) gspK 1107369..1108352 (+) 984 WP_001058507.1 type II secretion system minor pseudopilin GspK -
  I6J84_RS05275 (I6J84_05275) gspL 1108367..1109530 (+) 1164 WP_001335091.1 type II secretion system protein GspL -
  I6J84_RS05280 (I6J84_05280) gspM 1109527..1109988 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  I6J84_RS05285 (I6J84_05285) pilD 1109988..1110665 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  I6J84_RS05290 (I6J84_05290) bfr 1110694..1111170 (-) 477 WP_000675504.1 bacterioferritin -
  I6J84_RS05295 (I6J84_05295) bfd 1111242..1111436 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J84_RS05300 (I6J84_05300) chiA 1111605..1114307 (-) 2703 WP_000773170.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=467077 I6J84_RS05285 WP_000178175.1 1109988..1110665(+) (pilD) [Escherichia coli strain FDAARGOS_1254]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=467077 I6J84_RS05285 WP_000178175.1 1109988..1110665(+) (pilD) [Escherichia coli strain FDAARGOS_1254]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387