Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H0S59_RS01820 Genome accession   NZ_CP059078
Coordinates   384064..385776 (-) Length   570 a.a.
NCBI ID   WP_031952279.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain WM98B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 379064..390776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S59_RS01790 (H0S59_01790) - 379228..379554 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  H0S59_RS01795 (H0S59_01795) rlmB 379656..380405 (+) 750 WP_002156969.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H0S59_RS01800 (H0S59_01800) - 380441..381355 (+) 915 WP_033846385.1 DMT family transporter -
  H0S59_RS01805 (H0S59_01805) coaE 381352..381948 (-) 597 WP_004883551.1 dephospho-CoA kinase -
  H0S59_RS19055 (H0S59_01810) pilD 381950..382810 (-) 861 WP_022575844.1 A24 family peptidase Machinery gene
  H0S59_RS01815 (H0S59_01815) pilC 382810..384036 (-) 1227 WP_033846386.1 type II secretion system F family protein Machinery gene
  H0S59_RS01820 (H0S59_01820) pilB 384064..385776 (-) 1713 WP_031952279.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H0S59_RS01825 (H0S59_01825) tpiA 386067..386861 (+) 795 WP_004710827.1 triose-phosphate isomerase -
  H0S59_RS01830 (H0S59_01830) secG 386874..387203 (+) 330 WP_002156913.1 preprotein translocase subunit SecG -
  H0S59_RS01845 (H0S59_01845) rimP 387675..388199 (+) 525 WP_002157009.1 ribosome maturation factor RimP -
  H0S59_RS01850 (H0S59_01850) nusA 388237..389721 (+) 1485 WP_002156952.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63469.90 Da        Isoelectric Point: 5.2413

>NTDB_id=466994 H0S59_RS01820 WP_031952279.1 384064..385776(-) (pilB) [Acinetobacter nosocomialis strain WM98B]
MSVLHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEEIPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=466994 H0S59_RS01820 WP_031952279.1 384064..385776(-) (pilB) [Acinetobacter nosocomialis strain WM98B]
ATGTCAGTATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCCAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACCATTTCTGCGGAGTTTGCTGAACCATATTTTGATCTAGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTCTATATGT
CGCCACCAGCAATCCAAGTAATATTGAAGCAATCGATGCAATCCGTTTTAACAGTAAATTGCTGGTTGAACCTGTTATTG
TCGAGCATCATAAGCTGGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAATACCTCAAGGTGATGAAGCTCCTATTGT
TAAATATATTAATAAGTTGCTTATTGATGCCATTCGTATGGGCGCGTCAGATTTACATTTTGAGCCTTATGAAAAGTCAT
ATCGGGTACGTTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCGCCTTTACAACTTGCAAACCGTTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGACGTATTAAACTTAAGCTGTCAAA
AACCAAAGCAATTGATTTCCGTGTGAACTCGCTCCCTACCTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGCATTGATGCATTAGGCTACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTGATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTCAATCCTA
AAGTGGGTTTAACGTTTGCAGCTGCCCTTAAGTCTTTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAAGCTGCTCAAACCGGCCATATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTTTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCGGTAAATCTAGTGATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACATCCAGATTTTCGGGTTTTTCAACCCGTGGGCTGTCCTGAATGTCGTGAAGGTTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.596

100

0.986
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.617

99.474

0.504
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.579

90.877

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365