Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HZ993_RS21135 Genome accession   NZ_CP059068
Coordinates   4511569..4513302 (-) Length   577 a.a.
NCBI ID   WP_209394669.1    Uniprot ID   A0A975EA62
Organism   Rhodoferax sp. AJA081-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4506569..4518302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ993_RS21105 (HZ993_21060) - 4507297..4507782 (+) 486 WP_209394664.1 NUDIX domain-containing protein -
  HZ993_RS21110 (HZ993_21065) - 4507834..4508043 (-) 210 WP_209398666.1 DNA gyrase inhibitor YacG -
  HZ993_RS21115 (HZ993_21070) zapD 4508090..4508845 (-) 756 WP_209394665.1 cell division protein ZapD -
  HZ993_RS21120 (HZ993_21075) coaE 4508868..4509479 (-) 612 WP_209394666.1 dephospho-CoA kinase -
  HZ993_RS21125 (HZ993_21080) pilD 4509482..4510330 (-) 849 WP_209394667.1 A24 family peptidase Machinery gene
  HZ993_RS21130 (HZ993_21085) pilC 4510330..4511547 (-) 1218 WP_209394668.1 type II secretion system F family protein Machinery gene
  HZ993_RS21135 (HZ993_21090) pilB 4511569..4513302 (-) 1734 WP_209394669.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HZ993_RS21140 (HZ993_21095) - 4513459..4514907 (+) 1449 WP_209394670.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  HZ993_RS21150 (HZ993_21105) - 4515043..4515972 (-) 930 WP_209398668.1 polyprenyl synthetase family protein -
  HZ993_RS21155 (HZ993_21110) rplU 4516181..4516492 (+) 312 WP_209394671.1 50S ribosomal protein L21 -
  HZ993_RS21160 (HZ993_21115) rpmA 4516497..4516754 (+) 258 WP_209394672.1 50S ribosomal protein L27 -
  HZ993_RS21165 (HZ993_21120) cgtA 4516895..4517965 (+) 1071 WP_209394673.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62542.91 Da        Isoelectric Point: 6.2105

>NTDB_id=466974 HZ993_RS21135 WP_209394669.1 4511569..4513302(-) (pilB) [Rhodoferax sp. AJA081-3]
MATIDPLQRDSQAIALPGLGRALIAAGKLGQKSAEDLYRKALSSKNSFIAELMSSGAVSAADLAHTLSAAFAAPLLDLDA
VDTNRLPKGLLDSKICIAYRVVVLSKRSNRLIVATADPSDQAAAEKIKFSTQMGVDWVIAEYDKLSKLVDANATTATEAM
EDIIGGDFEFDESAAEAVIDNGQANASEVEDAPVVRFLHKMLMDAFSMRASDLHFEPYEHTYRVRFRVDGELREIASPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLKAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPATLTRMRNMGIAAFNIASSVILITAQRLARRLCANCKVHVDIPR
EALLNAGFTEAQLDGSWKPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSSLDIARQSELEGVRSLRQSGLSK
VKLGMTSLEEVLGVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=466974 HZ993_RS21135 WP_209394669.1 4511569..4513302(-) (pilB) [Rhodoferax sp. AJA081-3]
ATGGCCACCATCGATCCCCTACAACGTGACAGTCAAGCCATAGCACTCCCAGGTCTAGGAAGGGCTTTGATCGCCGCGGG
CAAACTGGGGCAAAAGTCTGCAGAAGACCTCTACCGCAAGGCGCTCTCCAGCAAGAATAGCTTCATCGCCGAGTTGATGA
GCTCGGGAGCCGTGTCCGCGGCCGACCTTGCACATACTCTGTCCGCTGCATTCGCAGCACCGCTGCTGGATCTGGATGCG
GTGGACACCAACCGCTTGCCCAAAGGACTTTTGGACAGCAAGATCTGTATCGCCTACCGCGTTGTTGTCCTCAGTAAACG
CAGTAACCGTCTGATTGTTGCAACGGCCGACCCTTCCGACCAGGCAGCGGCTGAGAAGATCAAATTTTCGACCCAAATGG
GCGTGGATTGGGTGATCGCGGAGTACGACAAGTTGTCCAAGCTGGTGGATGCCAACGCCACCACCGCCACCGAAGCGATG
GAAGACATCATCGGCGGTGACTTCGAATTCGATGAATCCGCAGCAGAGGCCGTTATCGACAACGGCCAAGCCAATGCTTC
CGAAGTGGAAGACGCGCCTGTTGTGCGTTTTCTGCACAAGATGCTGATGGACGCATTCAGCATGCGGGCTTCCGACCTGC
ACTTTGAGCCCTATGAACATACGTACCGGGTGCGGTTTCGTGTGGACGGTGAATTGCGCGAAATTGCCTCGCCGCCGATT
GCCATCAAAGACAAGCTCGCCTCGCGGATCAAGGTGATCTCGCGCATGGATATTTCAGAAAAACGCGTGCCACAAGACGG
CCGCATGAAACTGAAAGTTGGTCCGGACCGTGTGATCGACTTCCGGGTCAGCACCCTGCCTACGTTATTTGGCGAAAAAA
TCGTGATCCGGATTTTGGATCCCAGCAGCGCAAAATTGGGGATCGACGCTTTGGGCTACGAGCCTGAGGAAAAGGAACGT
CTGCTCAAAGCGATTGGCCGTCCCTACGGCATGATTTTGGTCACGGGACCTACGGGCTCAGGCAAGACTGTGTCGCTGTA
CACCTGTCTGAATTTGTTGAATCAGCCAGGCGTCAACATTGCCACTGCCGAAGACCCTTCGGAAATCAACTTGCCCGGTG
TCAACCAGGTCAACGTGAACGAAAAGGCCGGGTTGACATTTGCGGCAGCGCTGAAATCTTTCCTGCGACAAGATCCCGAC
ATCATCATGGTCGGTGAAATTCGCGACCTGGAAACCGCTGATATTTCTATCAAAGCCGCGCAGACGGGGCATTTGGTGCT
GTCCACCCTGCACACCAACGATGCGCCAGCGACGCTGACCCGTATGCGGAATATGGGGATTGCAGCATTTAACATCGCGT
CCAGTGTGATTTTGATTACCGCACAGCGTCTGGCCAGGCGCCTGTGCGCCAACTGCAAAGTACATGTGGATATCCCCCGC
GAGGCGCTGCTCAATGCAGGCTTCACCGAGGCTCAATTGGACGGATCGTGGAAGCCCTACAAACCAGTAGGCTGCTCTGC
CTGCAACAACGGCTACAAAGGACGAGTCGGCATCTACCAGGTCATGCCCATCAGCGAAGAAATCCAACGCATCATTTTGC
GTGACGGTAGCTCACTCGACATCGCCCGACAATCAGAATTGGAAGGCGTGCGCTCCTTGCGACAATCGGGCTTGAGCAAA
GTCAAACTGGGAATGACTTCATTGGAAGAAGTTCTCGGCGTTACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.902

97.227

0.534
  pilF Neisseria gonorrhoeae MS11

53.641

97.574

0.523
  pilB Acinetobacter baylyi ADP1

53.476

97.227

0.52
  pilB Legionella pneumophila strain ERS1305867

51.146

98.267

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

44.624

96.707

0.432
  pilB Vibrio campbellii strain DS40M4

44.149

97.747

0.432
  pilB Vibrio cholerae strain A1552

46.402

91.508

0.425
  pilF Thermus thermophilus HB27

36.348

99.653

0.362