Detailed information NTG0466779

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: H0A38_RS11110
• Genome accession: NZ_CP059048
• Coordinates: 2255039..2255485 (-)
• Length: 148 a.a.
• NCBI ID: WP_165379808.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain LAC460
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2250039..2260485

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0A38_RS11075 (H0A38_11015)	-	2250158..2250535 (-)	378	WP_033900715.1	pyridoxamine 5'-phosphate oxidase family protein	-
H0A38_RS11080 (H0A38_11020)	-	2250739..2251605 (+)	867	WP_058204413.1	RluA family pseudouridine synthase	-
H0A38_RS11085 (H0A38_11025)	-	2251643..2252452 (-)	810	WP_014570791.1	metal ABC transporter permease	-
H0A38_RS11090 (H0A38_11030)	-	2252445..2253182 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
H0A38_RS11095 (H0A38_11035)	-	2253359..2254201 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
H0A38_RS11100 (H0A38_11040)	-	2254198..2254635 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
H0A38_RS11105 (H0A38_11045)	comGG	2254716..2255000 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
H0A38_RS11110 (H0A38_11050)	comGF	2255039..2255485 (-)	447	WP_165379808.1	competence type IV pilus minor pilin ComGF	Machinery gene
H0A38_RS11115 (H0A38_11055)	comGE	2255448..2255744 (-)	297	WP_033900709.1	competence type IV pilus minor pilin ComGE	Machinery gene
H0A38_RS11120 (H0A38_11060)	comGD	2255716..2256147 (-)	432	WP_226319344.1	competence type IV pilus minor pilin ComGD	Machinery gene
H0A38_RS11125 (H0A38_11065)	comGC	2256107..2256490 (-)	384	WP_012898622.1	competence type IV pilus major pilin ComGC	Machinery gene
H0A38_RS11130 (H0A38_11070)	comGB	2256504..2257577 (-)	1074	WP_080735950.1	competence type IV pilus assembly protein ComGB	Machinery gene
H0A38_RS11135 (H0A38_11075)	comGA	2257471..2258409 (-)	939	WP_033900707.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17039.84 Da
• Isoelectric Point: 9.0872

>NTDB_id=466779 H0A38_RS11110 WP_165379808.1 2255039..2255485(-) (comGF) [Lactococcus lactis strain LAC460]
MGRKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=466779 H0A38_RS11110 WP_165379808.1 2255039..2255485(-) (comGF) [Lactococcus lactis strain LAC460]
ATGGGAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATCTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	42.336	92.568	0.392
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378