Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HZU75_RS10580 Genome accession   NZ_CP058952
Coordinates   2228573..2230279 (-) Length   568 a.a.
NCBI ID   WP_180306041.1    Uniprot ID   A0A7D5ZGA0
Organism   Chitinibacter fontanus strain STM-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2223573..2235279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZU75_RS10550 (HZU75_10550) - 2223870..2224619 (-) 750 WP_180306036.1 GNAT family N-acetyltransferase -
  HZU75_RS10555 (HZU75_10555) - 2224814..2225008 (-) 195 WP_228028024.1 DNA gyrase inhibitor YacG -
  HZU75_RS10560 (HZU75_10560) zapD 2225005..2225763 (-) 759 WP_180306037.1 cell division protein ZapD -
  HZU75_RS10565 (HZU75_10565) coaE 2225788..2226411 (-) 624 WP_180306038.1 dephospho-CoA kinase -
  HZU75_RS10570 (HZU75_10570) pilD 2226417..2227283 (-) 867 WP_180306039.1 A24 family peptidase Machinery gene
  HZU75_RS10575 (HZU75_10575) pilC 2227332..2228558 (-) 1227 WP_180306040.1 type II secretion system F family protein Machinery gene
  HZU75_RS10580 (HZU75_10580) pilB 2228573..2230279 (-) 1707 WP_180306041.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HZU75_RS10585 (HZU75_10585) - 2230520..2231326 (-) 807 WP_180306042.1 inner membrane protein YpjD -
  HZU75_RS10590 (HZU75_10590) ffh 2231408..2232751 (+) 1344 WP_180306043.1 signal recognition particle protein -
  HZU75_RS10595 (HZU75_10595) rpsP 2232851..2233096 (+) 246 WP_157670806.1 30S ribosomal protein S16 -
  HZU75_RS10600 (HZU75_10600) rimM 2233107..2233652 (+) 546 WP_308419411.1 ribosome maturation factor RimM -
  HZU75_RS10605 (HZU75_10605) trmD 2233697..2234458 (+) 762 WP_180308793.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  HZU75_RS10610 (HZU75_10610) rplS 2234497..2234877 (+) 381 WP_180306044.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62548.15 Da        Isoelectric Point: 5.4363

>NTDB_id=466140 HZU75_RS10580 WP_180306041.1 2228573..2230279(-) (pilB) [Chitinibacter fontanus strain STM-7]
MSTPQISGLARLLVQHGRLSETDAEALQSAALSNKTTFIEQLTQSKKLTAREVAEFSAQTFGYPLLDLDRIDSSYLPQGL
LDNNVMRMQRLVPLFKRGTKLFIGLSDITNLEAVEKVRFQTNSQVEPIIVEDNKLIVLLNKTIEATGASLKDMVIDEADL
ALEGGEQEPSAAETQMGQDIDDAPVVKYLQKMLMDAINTGASDIHFEPYEKFYRIRFRVDGVLREIAQPPLAIKEKISSR
IKVISKLDISEKRIPQDGRMKLVLSKSRAIDFRVSTLPTMHGEKIVMRILDPSSATLGIDALGYDPDQKAIILDAIQRPY
GMVLVTGPTGSGKTVSLYTCLNLLNKPDINISTAEDPCEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIQPFNIASSVILITAQRLARRLCSCKKHVEIPPQAMLDAGFTQ
EDLDGTWEPYGPTGCDLCKGTGYKGRVGIYQVMPITDEINRLIMNHGNAIDIADQARREGVLSLREAGLRKVKQGLTSLE
EVMAVTNI

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=466140 HZU75_RS10580 WP_180306041.1 2228573..2230279(-) (pilB) [Chitinibacter fontanus strain STM-7]
ATGAGCACCCCGCAAATTTCCGGCCTCGCCCGTTTGTTGGTCCAACACGGACGACTAAGCGAAACCGACGCTGAAGCGTT
GCAGTCGGCCGCCCTTAGCAATAAAACCACTTTCATTGAGCAGCTAACCCAAAGCAAGAAACTGACTGCACGCGAAGTTG
CGGAGTTTTCCGCGCAAACATTTGGCTACCCGCTACTCGACCTTGATCGAATTGATTCCAGCTACTTACCTCAAGGTCTG
CTAGACAACAACGTCATGCGCATGCAGCGCCTCGTGCCATTATTTAAGCGTGGCACCAAGCTATTCATTGGTCTTTCCGA
CATCACCAACCTTGAAGCGGTTGAAAAAGTCCGGTTTCAAACCAACTCACAAGTTGAACCCATTATTGTCGAAGACAATA
AACTAATTGTCCTGCTCAATAAAACCATTGAGGCAACTGGTGCCAGCCTCAAAGACATGGTGATCGACGAAGCTGACCTT
GCCCTAGAAGGCGGCGAACAAGAACCGAGTGCCGCGGAAACCCAAATGGGCCAAGATATTGACGATGCACCTGTCGTCAA
ATATCTGCAAAAGATGCTGATGGATGCCATCAACACCGGCGCCTCAGATATCCACTTTGAGCCTTATGAGAAATTTTATC
GCATCCGCTTTAGGGTCGACGGCGTATTACGCGAGATTGCCCAACCCCCGCTGGCGATCAAAGAAAAAATCTCATCGCGG
ATTAAAGTAATTTCTAAGTTGGATATTTCAGAGAAACGCATCCCTCAAGATGGTCGTATGAAGTTAGTGCTATCCAAATC
GCGGGCAATCGACTTCCGGGTTTCCACACTTCCCACCATGCATGGTGAGAAGATTGTAATGCGTATTTTGGATCCATCAT
CTGCGACACTCGGCATTGATGCTCTCGGCTACGACCCAGACCAAAAAGCCATTATTCTTGACGCTATTCAGCGCCCTTAT
GGGATGGTTCTAGTTACCGGACCAACAGGCTCGGGTAAAACCGTATCTCTGTATACTTGCTTAAATTTACTAAATAAGCC
CGATATTAATATCTCCACAGCGGAAGATCCCTGTGAGATCAACTTACCTGGCATCAACCAAGTCAATGTCAACGACAAAG
CAGGGCTGACCTTCTCTGCCGCACTGAAGGCCTTCTTACGGCAAGATCCAGATATTATCATGGTAGGTGAGATCCGTGAT
TTGGAAACTGCCGACATTGCAATTAAGGCCGCCCAAACTGGGCACATGGTTTTTTCAACCCTACACACGAACGATGCGCC
AACAACGTTAACCCGGATGCTCAATATGGGCATTCAACCGTTTAATATTGCTTCGTCAGTTATTTTGATTACCGCCCAGC
GCCTAGCTCGTCGTCTATGTAGCTGCAAAAAACATGTTGAAATTCCACCTCAAGCAATGCTTGATGCTGGTTTTACTCAG
GAAGATCTCGATGGAACCTGGGAACCCTATGGTCCGACTGGCTGCGATTTGTGCAAAGGTACGGGCTACAAAGGACGGGT
AGGCATTTACCAAGTCATGCCGATTACCGACGAAATCAATCGACTTATTATGAACCACGGCAATGCAATTGATATTGCCG
ATCAAGCCCGTCGCGAAGGGGTACTCAGCCTACGCGAGGCGGGCCTTCGCAAAGTCAAACAAGGCCTAACCTCACTAGAA
GAGGTAATGGCAGTAACAAATATCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5ZGA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.833

100

0.549
  pilF Neisseria gonorrhoeae MS11

54.577

100

0.546
  pilB Acinetobacter baylyi ADP1

53.616

99.824

0.535
  pilB Legionella pneumophila strain ERS1305867

52.098

100

0.525
  pilB Vibrio cholerae strain A1552

47.359

100

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

99.296

0.452
  pilB Vibrio campbellii strain DS40M4

44.681

99.296

0.444
  pilF Thermus thermophilus HB27

38.313

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

92.782

0.371