Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU825_RS07375 Genome accession   NZ_CP058908
Coordinates   1559330..1561474 (+) Length   714 a.a.
NCBI ID   WP_234303219.1    Uniprot ID   -
Organism   Pseudomonas phenolilytica strain RBPA9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1554330..1566474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU825_RS07355 (HU825_07345) pilM 1556494..1557558 (+) 1065 WP_043297919.1 pilus assembly protein PilM Machinery gene
  HU825_RS07360 (HU825_07350) pilN 1557558..1558130 (+) 573 WP_043297920.1 type 4a pilus biogenesis protein PilN -
  HU825_RS07365 (HU825_07355) pilO 1558127..1558750 (+) 624 WP_043297921.1 type 4a pilus biogenesis protein PilO -
  HU825_RS07370 (HU825_07360) pilP 1558747..1559274 (+) 528 WP_043297922.1 type 4a pilus biogenesis lipoprotein PilP -
  HU825_RS07375 (HU825_07365) pilQ 1559330..1561474 (+) 2145 WP_234303219.1 type IV pilus secretin PilQ Machinery gene
  HU825_RS07380 (HU825_07370) aroK 1561482..1562027 (+) 546 WP_043297924.1 shikimate kinase AroK -
  HU825_RS07385 (HU825_07375) aroB 1562048..1563151 (+) 1104 WP_043297925.1 3-dehydroquinate synthase -
  HU825_RS07390 (HU825_07380) - 1563161..1564714 (+) 1554 WP_234303220.1 AAA family ATPase -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 76825.11 Da        Isoelectric Point: 4.8562

>NTDB_id=465928 HU825_RS07375 WP_234303219.1 1559330..1561474(+) (pilQ) [Pseudomonas phenolilytica strain RBPA9]
MNSTVSRLGVALLALLAAPALLAANLNALDVAALPGDRVELKLAFDEPVNKPHGYTLEQPARIALDLPGVSNKLGVKNRE
LGVGNARSVTVVEAQGRTRLIVNLTTLVPYSTRIEGSNLFVVLGESSAPVAVSNSAVAGPVATPAPAKPLLTSKAINNID
FRRGEDGAGNVIITLSDPSISSNIEEQSGKIHVSFAKTQLPEALRVRLDVQDFATPVKFVDATTQGGGAAIAIEPTGRYD
YLAYQTDDKLTISIKPLTEDEAQKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIADLAPLRREVVQVNYAKAADIARLFQSVTDKDGASE
DRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWAGDKWNAWGKNGQL
GVADDPDASGKPGDDGYVPSRQVGRFPGIPEVPMNSPFVDMGAVGATSGIGLGFITDNAILDLQLSAMEKTGNGEVVSQP
KVVTADKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAASTGGIPAIRKN
EVNAKVLVSDGETIVIGGVFSNTQSKSVDKVPFLGDLPFIGRVFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=465928 HU825_RS07375 WP_234303219.1 1559330..1561474(+) (pilQ) [Pseudomonas phenolilytica strain RBPA9]
ATGAATAGCACCGTCTCGCGTCTTGGTGTCGCATTGTTGGCGCTCCTGGCCGCCCCGGCTCTGCTGGCTGCCAACCTCAA
CGCGTTGGATGTTGCAGCGCTGCCCGGTGATCGTGTCGAACTCAAGCTGGCGTTCGACGAGCCGGTGAATAAGCCACATG
GCTATACGCTCGAGCAGCCGGCGCGCATTGCGCTGGATTTGCCCGGCGTGTCCAACAAGCTGGGTGTGAAGAATCGTGAG
TTGGGAGTAGGTAATGCTCGCAGCGTTACGGTTGTTGAGGCGCAAGGGCGTACCCGTCTGATCGTCAACCTGACGACCCT
GGTGCCCTACTCGACGCGCATCGAGGGCAGCAACCTGTTCGTTGTGCTCGGCGAATCCTCGGCGCCGGTTGCCGTATCCA
ATTCCGCCGTGGCTGGCCCGGTAGCTACGCCAGCGCCTGCTAAGCCACTGCTAACGAGCAAGGCAATCAACAACATCGAT
TTTAGGCGCGGCGAAGATGGCGCCGGTAATGTAATCATCACGCTTTCCGATCCTTCGATCAGTTCGAATATCGAAGAGCA
GAGCGGGAAGATCCACGTTTCGTTTGCCAAAACTCAGTTGCCTGAGGCTTTGCGGGTTCGTCTGGATGTGCAGGATTTCG
CTACTCCAGTGAAGTTCGTCGATGCGACTACACAGGGTGGTGGTGCAGCTATCGCCATTGAACCTACAGGTCGCTACGAC
TATCTGGCCTATCAGACCGATGACAAGCTGACGATCAGCATCAAACCGCTGACCGAAGATGAGGCGCAGAAACGCAAGGC
AGAGCGCTTTGCCTATTCGGGCGAGAAATTGTCGCTCAACTTCCAGGACATTGATGTCCGCTCGGTACTGCAGTTGATTG
CAGACTTTACCGATCTCAATTTGGTCGCCAGCGATACGGTGCAGGGCAACATCACGCTACGGTTGCAGAATGTGCCGTGG
GACCAGGCTCTTGATCTGGTCCTCAAAACCAAGGGGCTGGACAAGCGGCAGGTTGGCAATGTGCTGCTGGTCGCGCCGGC
TGATGAGATTGCAGCGCGCGAGCGCCAAGAACTTGAGTCGCAGCGGCAGATCGCGGATCTCGCGCCGCTGCGCCGAGAAG
TGGTCCAGGTTAATTACGCCAAGGCTGCCGATATCGCCCGACTGTTCCAGTCTGTAACTGACAAGGACGGCGCGAGCGAA
GATCGCGGGTCCATCGCAGTCGATGATCGCACCAACAACATTATTGCCTACCAGACGCAGGAGCGACTCGACGAGCTGCG
CCGCATCGTCGCGCAACTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGTATCGTCGAAGCCAACGTCGACTATG
ACAAGAGCCTGGGTGTGCGTTGGGGGGGCAACCTCTGGGCGGGCGACAAGTGGAACGCCTGGGGCAAGAATGGGCAGCTG
GGTGTAGCTGATGATCCGGATGCGAGCGGCAAGCCTGGCGATGATGGTTATGTGCCGTCCCGGCAGGTCGGGCGTTTCCC
TGGTATCCCGGAAGTGCCAATGAACAGCCCGTTTGTTGATATGGGGGCTGTTGGCGCAACGTCGGGTATCGGCTTGGGGT
TCATTACTGACAACGCGATTCTTGATCTGCAGTTGTCCGCTATGGAAAAGACTGGTAATGGTGAGGTCGTCTCGCAGCCG
AAGGTTGTTACGGCAGACAAGGAAACGGCCAAGATCCTGAAAGGTTCAGAGGTTCCCTATCAGGAGGCCAGCTCCAGCGG
CGCGACCAGCACGTCTTTCAAGGAAGCTGCGCTTTCACTGGAGGTGACGCCGCAGATCACTCCGGATAATCGCATCATCA
TGGAAGTCAAGGTAACCAAGGATGAGCCAGACTTCTCGCAAGCGGCGAGTACCGGCGGTATCCCAGCAATTCGCAAGAAT
GAAGTGAACGCCAAGGTGCTGGTCAGCGATGGTGAAACCATCGTGATTGGCGGCGTTTTCTCCAACACGCAGAGCAAGTC
GGTAGACAAGGTTCCGTTCCTCGGTGATCTACCGTTCATTGGTCGAGTGTTCCGCCGCGATCTGGTTCAAGATCGGAAGT
CCGAACTGCTGGTATTCATCACGCCACGGATCATGAATAATCAGGCGATCCAAGCCAGCCGCTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-122)

Bacterial type II and III secretion system protein (Secretin)

(547-705)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.802

100

0.742
  comQ Acinetobacter baylyi ADP1

40.954

99.86

0.409
  pilQ Acinetobacter baumannii strain A118

38.814

100

0.403
  pilQ Acinetobacter baumannii D1279779

38.814

100

0.403
  pilQ Legionella pneumophila strain ERS1305867

37.41

97.339

0.364
  pilQ Legionella pneumophila strain Lp02

37.356

97.479

0.364